改変型アマドリアーゼ及びその製造法、並びにアマドリアーゼの界面活性剤耐性向上剤及びこれを用いたＨｂＡ１ｃ測定用組成物

Japanese Patent JP7158108

B2

Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.

鉞 陽介
Atsushi Ichiyanagi

JP2020003038A

October 21, 2022

January 10, 2020

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Kikkoman Corporation

C12Q1/28; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12N15/52; C12P21/02; G01N33/72

JP2011229526A

WO2012173185A1
WO2010041715A1
WO2005049858A1
WO2013162035A1

Patent Attorney Corporation Hiraki International Patent Office

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