To obtain the subject enzyme having a specific amino acid sequence, a monogalactosyl diacylglycerol(MGDG) synthase found abundantly in plants, etc., useful for improving the lipid composition of organisms, esp. controlling the quantity of MGDG.
This enzyme is a new monogalactosyl diacylglycerol(MGDG) synthase as a galactolipid found abundantly in plants, etc., having an amino acid sequence of the formula or that virtually identical with the above-mentioned amino acrid sequence. This enzyme is useful for improving the lipid composition of animals, plants or microorganisms owing to its gene, esp. for controlling the quantity of MGDG. This MGDG synthase is obtained by a process wherein cucumber seeds are allowed to germinate, a mRNA is separated from the resultant greened cotyledons; using the mRNA, a cDNA library is constructed and then retrieved using a synthetic DNA probe coding a partial peptide sequence estimated from the purified enzyme, and the resultant gene is integrated into an expression vector and expressed in host cells.
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SHIMOJIMA MIE
IWAMATSU AKIHIKO
TAKAMIYA KENICHIRO
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