To obtain the subject new MurF comprising an isolated polynucleotide containing an amino acid sequence having high identity of a specific amino acid sequence along the whole length, useful for searching an antibacterial compound, treating and diagnosing an infection with a bacterium of' the genus Streptococcus, etc.
This new MurF polypeptide comprises an isolated polypeptide containing an amino acid sequence having at least 70% identity of an amino acid sequence of the formula along the whole length of the formula and is useful for screening an antibacterial compound, treating and diagnosing an infection with Streptococcus aureus WCUH29, etc. The polypeptide is obtained by probing the library of a chromosome DNA clone of Streptococcus aureus WCUH29 in Escherichia coil or another proper host with a partial sequence- derived radioactive labeled oligonucleotide, preferably an oligonucleotide of heptadecamer or longer than it, incorporating the obtained MurF polynucleotide to an expression system and expressing the polynucleotide in a host cell.
SMITHKLINE BEECHAM PLC
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