PURPOSE: To obtain cholesterol oxidase from the interior of a mold efficiently, by using an anionic surface active agent as a culture solution of a bacterium producing cholesterol oxidase.
CONSTITUTION: An anionic surface active agent or it and bile acid or its salt are added to a culture solution obtained by cultivating a bacterium producing cholesterol oxidase (COD) in a growth medium or a solution suspending the mold collected from the culture solution, and cholesterol oxidase is extracted out of the mold. A bacterium producing and accumulating COD within the mold is used as the bacterium producing COD, for example, a bacterium belonging to the genus Streptomyces may be cited. COD extracted out of the mold is separated from the bacterium by centrifugation, or filtration, and obtained as a supernatant liquid or filtrate. COD is concentrated by ultrafiltration or salting out, so that the surface active agent used can be easily removed out of the system, and a product is prepared.
YAGE SHIGEJI
SATOMURA YOSHIO
ANDOU MINORU
Next Patent: PRODUCTION OF ENZYME-CONTAINING GRANULE