To obtian the subject blood stem cell useful for treating disorders after a superhigh administration dose chemotherapy using a large dose of an anticancer medicine aiming a therapy for annihilating cancer cells by bringing peripheral blood stem cells with into contact with a factor capable of stimulating gp 130.
This method for producing new peripheral blood stem cells comprises bringing peripheral blood stem cells comprising peripheral blood- originated CD 34 positive cells into contact with interleukin-6 (IL-6) or interleukin-6 receptor (IL-6R) as a factor capable of stimulating gp 130 and further interleukin-3 (IL-3) and stem cell factor(SCF) in the wells of a 96 hole plate. When all of IL-6, IL-6R, IL-3 and SCF are added, the most amplified peripheral blood stem cells are obtained as understood from cell numbers expressed by void bars expressing 8-50 cells/well, black-colored bars expressing 51-500 cells/well and oblique line bars expressing ≥501 cells/well.
KIMURA TAKAFUMI
YASUKAWA KIYOSHI
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