PURPOSE: To provide a method for efficient production of a Pasteuria bacterium which is a natural enemy of plant parasitic nematoda causing a damage to crops.
CONSTITUTION: A nematode having a Pasteuria bacterium adhering thereto is concentratively inoculated on a seedling of a dwarf Solanaceae plant (preferably tomato) or a dwarf legume (preferably bean) respectively cultured in a vessel which is separated into ≤100cm2 sections to produce the Pasteuria bacteria. The number of the inoculated nematode individuals is preferably 5000 to 25000 per one plant individual. If the case of a Solanaceae plant is compared with the case of a legume, the case of a Solanaceae plant requires an about three times longer period till the stage suitable for inoculation of nematode and exhibits a more excellent productivity per one plant individual than the case of a legume. As the amount of soil charged in a vessel used for culture of the plant, 100 to 300ml is suitable.
NISHIMUTA KOICHI
KAMOTO TAKANORI
YOSHIDA TOSHIAKI