PURPOSE: To obtain the subject product containing a small amount of oligosaccharide and possible to be readily purified in high degree by affecting liquefied enzyme, combination of β-amylase and pullulanase, liquefied enzyme and specific enzyme in turn to starch a above ground.
CONSTITUTION: At first, aqueous solution of starch above ground of 10-30wt.% concentration is liquefied by liquefied enzyme and said enzyme is deactivate by ≤10equiv. dextrose. Next, said liquefied matter is saccharified by β-amylase and pullulanase and/or isoamylase, then, further liquefied enzyme is added in an amount of 1-20 unit per substrate solid after initiation of saccharization and the saccharization is performed. At last, an enzyme produced by inserting code part of maltogenic-α-amylase of Bacillus stearothermophilus gene to plasmid and combining resultant matter to Bacillus subtilis is used to affection in an amount of 1-20 unit per 1g of substrate solid to afford the aimed substance containing 75-88% maltose of the solid component and ≤8 content of oligosaccharide calculated with the formula.
HARIO YUKARI
ISHII YOSHIFUMI
KATAURA KOICHI
KATO KAZUAKI
