Title:
PURIFICATION OF WATER-INSOLUBLE GLUCAN
Document Type and Number:
Japanese Patent JP3687194
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To efficiently purity the subject compound useful for food raw materials, artificial skins, anticancer agents, etc., at a low cost by simultaneously treating the culture solution of a microorganism producing the water-insoluble glucan, etc., with a specific amount of an oxidizing agent and a hydroxide compound capable of converting the pH of the solution to a basic region.
SOLUTION: The culture solution of a microorganism producing a water- insoluble glucan such as lentinan, yeast glucan or cellulose or a microorganism cell-containing solution containing the water-insoluble glucan as a constituting component is simultaneously treated with 0.175-3.5W/W% of an oxidizing agent (e.g. hydrogen peroxide) and a hydroxide compound capable of converting the pH of the solution to 10-12.5 (e.g. sodium hydroxide) to industrially, inexpensively and efficiently purify the objective water-insoluble glucan for food raw materials, artificial skins or paper as natural fibers, and further for antitumor agents, etc. The microorganism content of the culture solution or the microorganism cell-containing solution is 2.0×106 to 4.0×109cells/ml.
More Like This:
Inventors:
Kusatsu Masayoshi
Tanegawa Tetsuo
Shigeru Miyashiro
Tanegawa Tetsuo
Shigeru Miyashiro
Application Number:
JP14412696A
Publication Date:
August 24, 2005
Filing Date:
June 06, 1996
Export Citation:
Assignee:
AJINOMOTO CO.,LTD.
International Classes:
C08B1/00; C08B37/00; C12P19/04; C12R1/02; C12R1/645; C12R1/865; (IPC1-7): C12P19/04; C08B1/00; C08B37/00
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Ishii Teiji