PURPOSE: To obtain the title growth factor useful for research and measuring tracer, having physiological activity, biological and chemical stability by labeling tyrosine residue at the 24position and 60-position of insulin-like growth factor.
CONSTITUTION: Insulin-like growth factor I (IGF-I), an insulin-like peptide existing in blood, is prepared by gene recombination technology, peptide synthesis method or cell culture method, etc., put in a polypropylene tube, successively incorporated with 125I-containing sodium iodide, lactoperoxidase and hydrogen peroxide, reacted at 25°C for 10 minutes, the reaction is stopped by adding sodium azide, the reaction solution is subjected to reversed phase high performance liquid chromatography, desalted, then subjected to high performance liquid chromatography and purified to give the objective radioactive iodine labeled insulin-like growth factor I containing tyrosine residue at the 24 position and 60-position of IGF-I labeled with radioactive iodine.
KOHAMA YASUHIRO
OKABE MASARU
TSUJIKAWA KAZUTAKE
NODA YASUYO
