PURPOSE: To enable determination of triglyceride without being negatively affected by bilirubin contained in body fluid, by adding a specified amt. of ferrocyanide ions.
CONSTITUTION: In the reagent compsn. for determining triglyceride contg. ( I ) riboprotein lipase, (II) glycerol kinase and glycerophosphoric acid oxidase or (III) glycerol oxidase, (IV) peroxidase, (V) coupler phenazone, and (VI) an aniline deriv., ferrocyanide ions are added by 0.1W1.0μmol/l. Said ions are selected from sodium, potassium, and the like ferrocyanides, and the composition is preferably within 6.0W8.0 in pH. To determine triglyceride, a compsn. consisting of one, 2, or more solutions can be applied. In the case of 2 or more soln. type, said ferrocyanide ions may be added to either of the reagent soln. to be used before color development and that to be used during it.
MITSUHIDA NOBORU
NAKAGIRI YOSHITAKA
JPS5324893A | 1978-03-08 | |||
JPS4950991A | 1974-05-17 |
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