Title:
RESTRICTION-DEFICIENT MUTANT
Document Type and Number:
Japanese Patent JPH0576349
Kind Code:
A
Abstract:
PURPOSE: To conquer the restriction barrier of Bacillus thuringiensis and Bacillus cereus by proliferating a mutant having restriction barrier and using a transformed shuttle vector.
CONSTITUTION: A restriction deficient mutant of Bacillus thuringiensis and/or Bacillus cereus is obtained by proliferating a mutant having decreased restriction barrier by the several consecutive transformation and selection cycles, suitably using a transformed shuttle vector coding for different selection markers and guaranteeing sufficiently high transformation rate and selecting a mutant having decreased restriction barrier and keeping high transformation efficiency.
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Inventors:
BUARUTAA SHIYURUTAA
KURISUCHIINA RAINHARUTO
DANIERU MATE
KURISUCHIINA RAINHARUTO
DANIERU MATE
Application Number:
JP22965391A
Publication Date:
March 30, 1993
Filing Date:
August 16, 1991
Export Citation:
Assignee:
CIBA GEIGY AG
International Classes:
C12N1/21; C12N9/22; C12N15/09; C12N15/75; C12N1/20; C12R1/07; C12R1/085; (IPC1-7): C12N1/20; C12N1/21; C12N15/64; C12N15/75; C12N15/87
Attorney, Agent or Firm:
Nobuo Kaida (2 outside)