Title:
細胞分化のためのマーカーとしての分泌タンパク質
Document Type and Number:
Japanese Patent JP2009513106
Kind Code:
A
Abstract:
Provided are means and methods for in vitro and in vivo detection of chemically induced effects on embryonic development and differentiation for the purpose of embryotoxicity/teratogenicity screening as well as for the identification of pharmaceuticals such as growth and tissue promoting factors based on differentiating pluripotent embryonic stem (ES) cells. The assays are based on the use of transgenic ES cells and non-human animals comprising such ES cells or derivatives thereof, wherein said ES cells are characterized by the differentiation-dependent expression of a secreted reporter protein; in particular secreted embryonic alkaline phosphatase (SEAP).
Inventors:
Aerich, Andreas
Bohren, Helibelt
Schwenberg, Silke
Bohren, Helibelt
Schwenberg, Silke
Application Number:
JP2006518152A
Publication Date:
April 02, 2009
Filing Date:
July 08, 2004
Export Citation:
Assignee:
AXIOGENESIS AG
International Classes:
C12N15/09; A01K67/027; A61K45/00; A61P43/00; C12N5/06; C12N5/10; C12N15/79; C12N15/82; C12N15/85; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50
Domestic Patent References:
| JPH06189770A | 1994-07-12 | |||
| JPH10507624A | 1998-07-28 | |||
| JP2001514883A | 2001-09-18 | |||
| JP2001523106A | 2001-11-20 | |||
| JP2002051782A | 2002-02-19 | |||
| JP2002541832A | 2002-12-10 |
Foreign References:
| WO2002051987A1 | 2002-07-04 | |||
| WO2003048345A1 | 2003-06-12 | |||
| WO2003018760A2 | 2003-03-06 |
Other References:
JPN6010025668; Ｐｒｏｃｅｅｄｉｎｇｓ ｏｆ ｔｈｅ Ｎａｔｉｏｎａｌ Ａｃａｄｅｍｙ ｏｆ Ｓｃｉｅｎｃｅｓ ｏｆ ｔｈｅ Ｕｎｉｔｅｄ Ｓｔａｔｅｓ ｏｆ Ａｍｅｒｉｃａ Ｖｏｌ． ８８， Ｎｏ． １０, 199105, ｐｐ． ４１３８-４１４２
Attorney, Agent or Firm:
Tomimasa Konishi
Tomoko Nakamura
Mikiharu Hagino
Tomoko Nakamura
Mikiharu Hagino