Title:
SENSING STRATEGY AND METHOD FOR NUCLEIC ACID DETECTION USING BIOSENSOR
Document Type and Number:
Japanese Patent JP2017205121
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method for detecting an analyte comprising a nucleotide sequence.SOLUTION: An oligonucleotide primer for amplifying a target nucleotide sequence, comprising: a 5' anchor region, the region being configured to hybridize to a nucleotide sequence outside of the target nucleotide sequence and is at least partially complimentary to the nucleotide sequence outside of the target nucleotide sequence; and a 3' extension region, the region being configured to hybridize to a portion of the target nucleotide sequence; and a bubble region located between the 5' anchor region and the 3' extension region. the bubble region comprising a sequence that is not complementary or only partially complementary to the target nucleotide sequence and is cleavable by a restriction enzyme.SELECTED DRAWING: None
Inventors:
JONATHAN OHALLORAN
Application Number:
JP2017132667A
Publication Date:
November 24, 2017
Filing Date:
July 06, 2017
Export Citation:
Assignee:
QUANTUMDX GROUP LTD
International Classes:
C12N15/09
Domestic Patent References:
| JP2008531040A | 2008-08-14 |
Attorney, Agent or Firm:
Yasuhiko Murayama
Shinya Mitsuhiro
Tatsuhiko Abe
Shinya Mitsuhiro
Tatsuhiko Abe