Title:
PROCESS FOR SEMISYNTHESIS OF HUMAN INSULIN
Document Type and Number:
Japanese Patent JPS5914798
Kind Code:
A
Abstract:
PURPOSE: To prepare human insulin from swine insulin, in high efficiency, by using water as the reaction solvent and acromobacter.protease I as the catalyst.
CONSTITUTION: Swine insulin is made to react with threonine having carboxyl group protected with substituted or unsubstituted alkyl or aralkyl group in water or in an aqueous solution containing a small amount of an organic solvent miscible with water, in the presence of lysyl endopeptidase (Acromobacter.protease I) produced by Acromobacter lyticus at 0W50°C and 4W10pH, preferably under nearly neutral condition, to effect peptide exchange and obtain a human insulin derivative having carboxyl-protected threonine at B30.
Inventors:
SAKATA YOSHITSUGU
SHINTANI AKINORI
MATSUO TETSUYA
SUGIYAMA HARUHIKO
MINAMII NOBUAKI
NAGAOKA JIYOUJI
SHINTANI AKINORI
MATSUO TETSUYA
SUGIYAMA HARUHIKO
MINAMII NOBUAKI
NAGAOKA JIYOUJI
Application Number:
JP12391782A
Publication Date:
January 25, 1984
Filing Date:
July 16, 1982
Export Citation:
Assignee:
WAKO PURE CHEM IND LTD
International Classes:
C12P21/02; (IPC1-7): C12P21/02