【発明の名称】組換え成熟型リソフスタフィンの発現

Japanese Patent JP2003512050

A

A portion of the lysostaphin gene of Staphylococcus simulans has been cloned and overexpressed in the cytoplasm of E. coli to yield lysostaphin, in the absence of preprolysostaphin and prolysostaphin, under the transcriptional control of an IPTG-inducible promoter and a ribosome binding site. IPTG induction of the transformed host cells produces intracellular, soluble, mature lysostaphin (27 kDa), in the complete absence of preprolysostaphin and prolysostaphin. The mature lysostaphin so formed dose not require post-translational modification. The mature lysostaphin so formed can be used treat and prevent staphylococcal infections.

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Katuri, Gunsham
Sharma, raffle

JP2001532186A

April 02, 2003

October 19, 1999

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Barat Biotech International Limited
Council of Scientific and Industrial Research (CSI)

C12N15/09; A61K48/00; A61P31/04; C07H21/04; C07K14/00; C12N1/00; C12N1/20; C12N1/21; C12N5/10; C12N9/48; C12N9/52; C12N15/57; A61K38/00; C12R1/44; (IPC1-7): C12N15/09; A61P31/04; C12N1/21; C12N9/48

JPH01503036A	1989-10-19
JPH01503036A	1989-10-19
JPH0436184A	1992-02-06
JPH01117790A	1989-05-10

WO1999005289A1	1999-02-04
EP0759473A1	1997-02-26
EP0759473A1	1997-02-26

JPN6009039373; Ｊ．　Ｂｉｏｌ．　Ｃｈｅｍ． Ｖｏｌ．２４５，　Ｎｏ．１８, 1970, ｐ．４８４２-４８４６
JPN6013015233; 社団法人日本生化学会編、「タンパク質ＶＩ-合成および発現-」、第１版第１刷、平成４年６月１５日発行、 , 19920615
JPN6013015234; Ｅｕｒ．Ｊ．Ｂｉｏｃｈｅｍ．，１９９８，Ｖｏｌ．２５３，Ｎｏ．３，ｐ．６７５-６８３ , 1998

Tadahiko Ito (2 outside)

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