Title:
【発明の名称】RNAバイナリ・プローブとリボザイムリガーゼを用いたRNA検定法
Document Type and Number:
Japanese Patent JPH08505531
Kind Code:
A
Abstract:
There are provided nucleic acid hybridization assays for RNA targets using RNA binary probes and a ribozyme ligase that is a stringent RNA-directed RNA ligase. Preferred assays include exponential amplification for signal generation. Tetrahymena ribozyme ligase is a preferred ligase for use in this invention. It may be tethered to hold it close to the ligation junction. One assay according to this invention is a "tethered ligase chain reaction." Also provided are kits for performing assays according to the invention.
Inventors:
Lizard, paul m
Taiagi, Sanjay
Randeglan, Wolf Dee
Kramer, Fred Earl
Zo Stack, Jack W
Taiagi, Sanjay
Randeglan, Wolf Dee
Kramer, Fred Earl
Zo Stack, Jack W
Application Number:
JP51632594A
Publication Date:
June 18, 1996
Filing Date:
January 14, 1994
Export Citation:
Assignee:
The Public Health Research Institute of the City of New York, Inc.
The General Hospital Corporation
The General Hospital Corporation
International Classes:
C12N15/09; C07H21/02; C12Q1/68; C12Q1/682; C12Q1/6862; C12Q1/6867; (IPC1-7): C12Q1/68; C07H21/02; C12N15/09
Attorney, Agent or Firm:
Masatake Shiga (2 outside)