Title:
【発明の名称】少数細胞集団を濃縮する方法
Document Type and Number:
Japanese Patent JPH11502106
Kind Code:
A
Abstract:
A method for enriching a whole blood sample for rare cells is disclosed. A blood sample (12) is layered onto Ficoll/Hypaque-1119 (14) and centrifuged to remove maternal erythrocytes (16) and the rest of centrifuged blood sample is passed through a gravity-biased, counterflow stabilized charge-flow separator apparatus (20) to separate out fetal nucleated erythrocytes (26) from other cellular components in the presence of an applied electrical field. Separated fetal nucleated erythrocytes (26) undergo ammonia hemolysis (30) to eliminate mature erythrocytes and enriched fetal nucleated erythrocytes are recovered.
Inventors:
Summons, David W
Manly michael
Wootermoren, Joseph Gee
Twiti, Garland Yi
Manly michael
Wootermoren, Joseph Gee
Twiti, Garland Yi
Application Number:
JP52705896A
Publication Date:
February 23, 1999
Filing Date:
March 07, 1996
Export Citation:
Assignee:
Bio Separations Incorporated
International Classes:
B01D17/02; B01D21/26; B01D37/00; B01D63/08; G01N33/48; C12M1/42; C12N5/02; C12S3/22; G01N27/26; G01N27/447; G01N33/49; B01D17/04; (IPC1-7): C12N5/02; C12M1/42; G01N33/48; G01N33/49
Attorney, Agent or Firm:
Kazuo Shamoto (5 outside)