Title:
変異型イソプロピルリンゴ酸イソメラーゼ
Document Type and Number:
Japanese Patent JP4257215
Kind Code:
B2
Abstract:
A polypeptide has an amino acid sequence derived from the amino acid sequence of isopropylmalate isomerase originating in a microorganism belonging to coryneform bacteria by deletion, substitution or addition of some amino acids, which is the sole isopropylmalate isomerase produced by such microorganism that exhibits a partial leucine requirement in the minimum medium, is new. Independent claims are also included for: (1) a polypeptide having an amino acid sequence derived from the amino acid sequence of isopropylmalate isomerase large subunit (ISOM) originating in a microorganism belonging to coryneform bacteria by deletion, substitution or addition of some amino acids, which is optionally the sole isopropylmalate isomerase produced by such microorganism that exhibits a partial leucine requirement in the minimum medium; (2) a polypeptide with an amino acid sequence corresponding to that of the coryneform bacterium-originated ISOM amino acid sequence of (I) with 481 amino acids except the glycine residue at position-456 being replaced by other amino acid; (3) a similar polypeptide with an amino acid sequence corresponding to that of the coryneform bacterium-originated ISOM amino acid sequence of (I) with 481 amino acids except the glycine residue at position-456 being replaced by 1 or more of other amino acids; (4) another polypeptide derived from any of the already-specified polypeptides but with some amino acids added; (5) DNAs encoding the polypeptides; (6) a DNA containing a base sequence with a codon encoding the ISOM polypeptide with the glycine residue at position-456 replaced , which includes bases from 1366-1368 from the 5'-terminal in the sequence of (III) with 1443 base pairs; (7) a recombinant DNA hybridizable with a DNA with a base sequence complementary to that of sequence (III) under stringent conditions and encoding a coryneform bacterium-originated ISOM amino acid sequence with the corresponding glycine residue at position-456 substituted; (8) a microorganism transformed with any of the DNAs; (9) a similar microorganism that includes the already-specified DNA on its chromosome DNA; and (10) producing L-amino acids by culturing the transformant microorganisms before accumulation and collection of the product from the culture.
Inventors:
三橋 敏
中川 智
大西 淳子
林 幹朗
池田 正人
溝口 寛
中川 智
大西 淳子
林 幹朗
池田 正人
溝口 寛
Application Number:
JP2003576614A
Publication Date:
April 22, 2009
Filing Date:
March 19, 2003
Export Citation:
Assignee:
協和発酵バイオ株式会社
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88; C12N15/61; C12P13/04; C12P13/08; C12P13/10; C12P13/14; C12P13/22; C12P13/24; C12R1/13; C12R1/15