Title:
The depth filter in which it was improved for the disposable biotechnology method
Document Type and Number:
Japanese Patent JP6027104
Kind Code:
B2
Abstract:
A process for purifying a target protein from a sample comprising adding a precipitant to the sample and removing one or more impurities using a gradient density depth filter. The primary clarification of feeds, including chemically treated flocculated feeds, containing the target biomolecules of interest such a mAbs, mammalian cell cultures, or bacterial cell cultures, using a primary clarification depth filtration device without the use of a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarification depth filtration device contains a porous depth filter having graded porous layers of varying pore ratings. The primary clarification depth filtration device filters fluid feeds, including chemically treated flocculated feeds containing flocculated cellular debris and colloidal particulates having a particle size distribution of approximately about 0.5 pm to 200 pm, at a flow rate of about 10 litres/m2/hr to about 100 litres/m2/hr. Kits and methods of using and making the same are also provided.
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Inventors:
Shin, Nuri Pen
Qian, Kuotsuk-Siyun
Qian, Kuotsuk-Siyun
Application Number:
JP2014519060A
Publication Date:
November 16, 2016
Filing Date:
June 29, 2012
Export Citation:
Assignee:
EMD Millipore Corporation
International Classes:
C12M1/00; B01D39/06; B01D39/14; B01D39/16; B01D39/18; C07K1/34; C07K1/36
Domestic Patent References:
JP2009508486A | ||||
JP2005263803A | ||||
JP2009534030A | ||||
JP2010528844A |
Foreign References:
WO2010074702A1 | ||||
WO2010074953A1 | ||||
WO2010074773A1 | ||||
WO2010151447A1 | ||||
WO2012054679A1 | ||||
WO2010017964A1 | ||||
US4073732 | ||||
US20060249455 |
Other References:
BioPharm,1996 Apr,9(4),p.35-41
Biotechnol.Prog.,2005,21,p.1552-7
Biotechnol.Prog.,2005,21,p.1552-7
Attorney, Agent or Firm:
Kawaguchi International Patent Office
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