核酸分析デバイス、及び核酸分析装置

Japanese Patent JP5260339

B2

An object of the present invention is to regularly align microparticles, on each of which a nucleic acid synthetase or a DNA probe capable of capturing a nucleic acid sample fragment is immobilized, on a support so as to improve throughput of nucleic acid analysis. The present invention relates to a method comprising immobilizing a nucleic acid synthetase, a DNA probe, or the like in advance to a microparticle, forming a pattern of metal pads each having a diameter smaller than the microparticle diameter with gold or the like on a support, and allowing a microparticle to be bound to the pads via a chemical bond. In addition, when the surfaces of microparticles are electrically charged, a pattern of metal pads each having a diameter equivalent to or larger than the microparticle diameter is formed with gold or the like on a support and a microparticle is allowed to be bound to the pads via a chemical bond. According to the present invention, many types of nucleic acid fragment samples can be regularly aligned at a high density and immobilized on a support. This allows high throughput analysis of nucleic acid samples. For example, if microparticles are immobilized at 1-micron pitches, a high density of 106 nucleic acid fragments/emm2 can be readily achieved.

JPS6170989	RECOMBINANT DNA AND ITS USE
JP4175489	A conjugate of a modified superantigen and a target search compound and use of the conjugate
JPH07112434	[Title of Invention] Luciferase Gene

Toshiro Saito
Kazushige Imai

JP2009018936A

August 14, 2013

January 30, 2009

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Hitachi High-Technologies Corporation

C12N15/09; C12M1/00; G01N21/64; G01N33/53; G01N33/543; G01N37/00

JP200577284A
JP10160737A
JP2005219152A
JP2008190937A
JP2008197108A

Science, 2008, 320(5872), pp.106-109

Manabu Inoue
Yuji Toda

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