Title:
VACCINE
Document Type and Number:
Japanese Patent JP2022031791
Kind Code:
A
Abstract:
To provide a dengue subunit vaccine against all the four serotypes DENV-1, DENV-2, DENV-3, DENV-4.SOLUTION: The present invention discloses a method for making dengue virus-like particles (VLPs) for vaccine preparation, including the steps of (a) introducing an expression vector into a cultured cell, where the expression vector includes a nucleic acid encoding a recombinant polypeptide comprising the EDIII domain of each of Dengue virus serotypes DENV-1, DENV-2, DENV-3, and DENV-4 linked to the N-terminal of HBsAg polypeptide and (b) recovering the dengue VLPs.SELECTED DRAWING: None
Inventors:
NAVIN KHANNA
VISWANATHAN RAMASAMY
VISWANATHAN RAMASAMY
Application Number:
JP2021191104A
Publication Date:
February 22, 2022
Filing Date:
November 25, 2021
Export Citation:
Assignee:
INT CENTRE FOR GENETIC ENGINEERING & BIOTECHNOLOGY
International Classes:
C12N7/04; A61K39/12; A61P31/14; A61P37/04; C12N1/15; C12N1/19; C12N5/10; C12N15/40; C12N15/51; C12N15/62
Foreign References:
| WO2008152652A2 | 2008-12-18 | |||
| US20130071419A1 | 2013-03-21 | |||
| CN101962411A | 2011-02-02 | |||
| WO2007034507A2 | 2007-03-29 |
Other References:
NIYATI KHETARPAL ET AL.: "Dengue-specific subviral nanoparticles: design, creation and characterization", J. NANOBIOTECHNOLOGY, vol. Vol.11, Article number 15, JPN6019023132, 2013, ISSN: 0004905052
HIMANI BISHT ET AL.: "Recombinant dengue virus type 2 envelope/hepatitis B surface antigen hybrid protein expressed in Pic", JOURNAL OF BIOTECHNOLOGY, vol. Vol.99, Issue 2, JPN6021021485, 2002, pages 97 - 110, ISSN: 0004905053
HIMANI BISHT ET AL.: "Recombinant dengue virus type 2 envelope/hepatitis B surface antigen hybrid protein expressed in Pic", JOURNAL OF BIOTECHNOLOGY, vol. Vol.99, Issue 2, JPN6021021485, 2002, pages 97 - 110, ISSN: 0004905053
Attorney, Agent or Firm:
Makoto Ono
Kanayama Sage
Yoshiyuki Tsubokura
Kazuki Shigemori
Kenji Ando
Hidehiko Ichikawa
Yoshie Sakurada
Yosuke Kawasaki
Youichi Iino
Yusuke Ichikawa
Masahiro Moriyama
Takashi Kono
Yoshikazu Iwase
Yasufumi Shiroyama
Kanayama Sage
Yoshiyuki Tsubokura
Kazuki Shigemori
Kenji Ando
Hidehiko Ichikawa
Yoshie Sakurada
Yosuke Kawasaki
Youichi Iino
Yusuke Ichikawa
Masahiro Moriyama
Takashi Kono
Yoshikazu Iwase
Yasufumi Shiroyama