Title:
6(R)-L-ERYTHRO-5,6,7,8-TETRAHYDROBIOPTERIN RECEPTOR
Document Type and Number:
WIPO Patent Application WO/1993/010155
Kind Code:
A1
Abstract:
A receptor (binding protein) specific for 6(R)-L-erythro-5,6,7,8-tetrahydrobiopterin which is efficacious against psychoneurosis such as autism. It is present in the cytosol or membrane fraction obtained from an animal organ homogenate, can be solubilized from the membrane fraction in the presence of CHAPS, or 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, and is characterized in that it loses most of the capacity of binding with 6R-BH4 when heated at 100 °C for 10 min, that it loses significantly the capacity of binding with 6R-BH4 when incubated with trypsin at 37 °C for 15 min, and that it has a relative molecular weight of around 340-380 kDa when analyzed by using a molecular sieve comprising a Cosmosil 5 Diol-300 column after being specifically combined with tritium-labeled 6R-BH4.
Inventors:
WATANABE YASUYOSHI (JP)
WATANABE YUMIKO (JP)
MORII HIROSHI (JP)
HAYASHI TOSHIO (JP)
WATANABE YUMIKO (JP)
MORII HIROSHI (JP)
HAYASHI TOSHIO (JP)
Application Number:
PCT/JP1992/001496
Publication Date:
May 27, 1993
Filing Date:
November 16, 1992
Export Citation:
Assignee:
SUNTORY LTD (JP)
OSAKA BIOSCIENCE INST (JP)
OSAKA BIOSCIENCE INST (JP)
International Classes:
C07K14/705; A61K38/00; (IPC1-7): C07K15/06
Other References:
CHEMICAL ABSTRACTS, Vol. 115, No. 19, November 11, 1991 (11.11.91), (Columbus, Ohio, USA), S.W. BAILEY et al., "Role of C6 chirality of tetrahydropterin cofactor in catalysis and regulation of tyrosine and phenylalanine hydroxylases", refer to Abstract No. 201845w, 2nd part, page 443, Biochemistry 1991, 30 (42), 10226-35 (Eng).
CHEMICAL ABSTRACTS, Vol. 115, No. 19, November 11, 1991 (11.11.91), (Columbus Ohio, USA), S. ICHIKAWA et al., "Expression of mouse tyrosine hydroxylase in Escherichia coli", refer to Abstract No. 2002245, 1st part, page 290, Biochem. Biophys. Res. Commun. 1991, 178 (2), 664-71 (Eng).
CHEMICAL ABSTRACTS, Vol. 115, No. 19, November 11, 1991 (11.11.91), (Columbus Ohio, USA), S. ICHIKAWA et al., "Expression of mouse tyrosine hydroxylase in Escherichia coli", refer to Abstract No. 2002245, 1st part, page 290, Biochem. Biophys. Res. Commun. 1991, 178 (2), 664-71 (Eng).
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