FIELD OF THE INVENTION The present invention is directed to 8-hydroxy-l-oxo-tetrahydropyrrolopyrazine compounds and pharmaceutically acceptable salts thereof, their synthesis, and their use as inhibitors of the SV integrase enzyme. The compounds of the present invention and their phanmaceutically acceptable salts are useful for preventing or treating infection by HJIV and for treating, delaying the onset of, or preventing AIDS.

BACKGROUND OF THE INVENTION A retrovirus designated human immunodeficiency virus (HIV) is the etiological agent of the complex disease that includes progressive destruction of the immune system (acquired immune deficiency syndrome ; AIDS) and degeneration of the central and peripheral nervous system. This virus was previously known as LAV, HTLV-HI, or ARV. A common feature of retrovirus replication is the insertion by viTally-encoded integrase of proviral DNA into the host cell genome, a required step in MV replication in human T- lymphoid and monocytoid cells-Integration is believed to be mediated by integrase in three steps : assembly of a stable nucleoprotein complex with viral DNA sequences ; cleavage of two nucleotides from the 3'termini of the linear proviral DNA ; covalent joining of the recessed 3' OH termini of the proviral DNA at a staggered cut made at the host target site.

The fourth step in the process, repair synthesis of the resultant gap, may be accomplished by cellular enzymes.

Nucleotide sequencing of HIV shows the presence of a pol gene in one open reading frame [Ratner et al., Nature 1985, 313: 277]. Amino acid sequence homology provides evidence that the pol sequence encodes reverse transcriptase, integrase and an HIV protease [Toh et al., EMBO J. 1985, 4 : 1267; Power et al., Science 1986, 231 : 1567, Pearl et al. , Nature 1987,329 : 351a. All three enzymes have been shown to be essential for the replication of HIV.

It is known that some antiviral compounds which act as inhibitors of HIV replication are effective agents in the treatment of AIDS and sirruiJar diseases, including reverse transcriptase inhibitors such as azidothymidine (AZT) and efavirenz and protease inhbitors such as indinavir and nelfinavir. The compounds of this invention are inhibitors of HIV integrase and inhibitors of HItV replication. The inhibition of integrase in vitro and of HIV replication in cells is a direct result of inhibiting the strand transfer reaction catalyzed by the recombinant integrase in vitro in HIY infected cells. A particular advantage of the present invention is highly specific inhibition of HIV integrase and HTV replication- The following references are of interest as background: Chemical Abstracts No. 33-2525 discloses the preparation of 5-chloro-8-hydroxy- 1, 6-naphthyridine-7-carboxylic acid amide from the corresponding methyl ester.

US 5,294, 620 discloses certain 1, 6-naphthyridin-2-one derivatives having angiotensin II antagonist activity.

US 2003/0055071 (Publication of U. S. Application Serial No. 09/973, 853, filed October 10, 2001) and WO 02/30930 (Publication of International Application No. PCT/US 01/31456, filed October 9,2001) each disclose certain 8-hydroxy-1, 6-naphthyridine-7- carboxamides as HVV integrase inhibitors, wherein the carboxamido nitrogen is directly or indirectly attached to phenyl or phenyl fused to a carbocycle-The carboxamides are disclosed to be useful, inter alia, for treating HIV infection and AIDS-WO 02/30426 discloses another group of 8-hydroxy-1, 6-naphiyridine-7-carboxanlides as HIV integrase inhibitors, wherein the carboxamido nitrogen is directly or indirectly attached to a heterocycle. WO 02/055079 discloses still another group of 8-hydroxy-1, 6-naphthyridine-7-carboxamides as HIV integrase inhibitors, wherein the carboxamido nitrogen is part of a heterocyclic ring system.

WO 02/036734 discloses certain aza-and polyaza-naphthalenyl ketones to be ST integrase inhbitors. The ketones include certain 1-aryl-1-(poly)azanaphthylenyl methanones and 1-heterocyclyl-1-(poly) azanaphthylenyl methanones. Quinolinyl, naphthyridinyl, and quinoxalinyl are disclosed as suitable (poly)azanaphthalenyl groups in the ketones.

WO 03/35076 discloses certain 5, 6-dihydroxypyriniidine-4-carboxamides as HIV integrase inhibitors, and WO 03/35077 discloses certain N-substituted 5-hydroxy-6-oxo-1, 6- dihydropytimidine-4-carboxamides as HIV integrase inhibitors.

SUMMARY OF THE RWENTION The present invention is directed to novel 8-hydroxy-l-oxo- tetrahydropyrrolopyrazine compounds. These compounds and their pharmaceutically acceptable salts are useful in the inhibition of HIV integrase, the prevention of infection by HIV, the treatment of infection by IItV and in the prevention, treatment, and delay in the onset of AIDS, either as compounds or their pharmaceutically acceptable salts, or as pharmaceutical composition ingredients, whether or not in combination with other HIV/AIDS antivirals, anti-infectives, immnnomodulators, antibiotics or vaccines. More particularly, the present invention includes a compound of Formula (I), or a pharmaceutically acceptable salt thereof : wherein RI is -H, -C1-6 alkyl, -C3-6 cycloalkyl, or -C1-6 alkyl which is substituted with 1 or 2 substituents each of which is independently : (1) C3-S cycloalkyi, (2) aryl, (3) a 5-or 6-membered saturated or mono-unsaturated heterocyclic ring containing from 1 to 4 heteroatoms independently selected from N, O and S, (4) a 5-or 6-membered heteroaromatic ring containing from 1 to 4 heteroatoms independently selected from N, O and S, or (5) a 9-or 10-membered fused bicyclic heterocycle containing from 1 to 4 heteroatoms independently selected from N, O and S, wherein at least one of the rings is aromatic ; wherein (A) each cycloalkyl is optionally substituted with frorn I to 3 substituents, each of which is independently halo, -C1-6 alkyl, or -O-C1-6 alkyl; (B) cach aryl is optionally substituted with from 1 to 5 substituents each of which is independently

(1)-CI-6 alkyl, optionally substituted with from 1 to 3 substituents each of which is independently -OH, -O-C1-6 alkyl, -O-C106 haloalkyl,-CN,-N02,-N (RaRb),-C (=O) N (RaRb),-C (=O) Ra, -CO2Rc, -S(O)nRc, -SO2N(RaRb), -N(Ra)C(=O) Rb, -N(Ra)CO2Rc, -N(Ra)SO2Rc, -N(Ra)SO2N(RaRb), -OC(=O)N(RaRb), or-N (Ra) C (=O) N (RaRb), (2) -O-C1-6 alkyl, optionally substituted with from 1 to 3 substituents each of which is independently -OH, -O-C1-6 alkyl, -O-C1-6 haloalkyl,-S (O) nRc,-C (=O) N (RaRb), -SO2N(RaRb), -N(Ra)C(=O)Rb, -N(Ra)CO2Rc, -N(Ra)SO2Rc, - N (Ra) S02N (RaRb),-OC (=O) N (RaRb), or -N(Ra)C(=O) N (RaRb), (3) -C1-6 haloalkyl, (4) -O-C1-6 haloalkyl, (5)-OR, (6) halo, (7)-CN, (8) -NO2, (9) -N(RaRb), (10) -C(=O) N (RaRb), <BR> <BR> <BR> (11)-C (=O) Ra,<BR> <BR> <BR> <BR> <BR> (12)-CO2RC,<BR> <BR> <BR> <BR> <BR> <BR> (13)-SRc, (14)-S (=O) Rc, (15)-S02RC, (16) -N(Ra)SO2Rc, (17)-S02N (RaRb), (18)-N (Ra) C (=O) Rb, or (19) -N(Ra)CO2Rc, (C) each saturated or mono-unsaturated heterocyclic ling is (i) optionally substituted with from 1 to 5 substituents each of which is independently halogen, -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo; and

(ii) optionally substituted with 1 or 2 substituents each of which is independently aryl or a 5- or 6-membered heteroaromatic ring containing from I to 4 heteroatoms independently selected from N, O and S; and (D) each heteroaromatic ring or each fused bicyclic heterocycle is (i) optionally substituted with from 1 to 7 substituents each of which is independently halogen,-C1 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo ; and (ii) optionally substituted with 1 or 2 substituents each of which is independently aryl or -C1-6 alkyl-aryl; R2 is-H or-Cl-6 alkyl ; R3 is -H, -C1-6 alkyl, -c1-6 haloalkyl, or -C1-6 alkyl substituted with one of-OH,-O-C1-6 alkyl, -O-C1-6 haloalkyl, -CH, -NO2, -N(RaRb), -C(=O)N(RaRb), -C(=O)Ra, -CO2Rc, -S(O)nRc, -SO2N(RaRb), -N(Ra)C(=O) Rb, -N (Ra) C02Rc,-N (Ra) So2Rc,-N (Ra)SO2N(RaRb), <BR> <BR> <BR> -OC (=O) N (RaRb), or-N (Ra) C (=O) N (RaPb) ;<BR> <BR> <BR> <BR> <BR> <BR> <BR> R4 is : (1) -H, (2)-C 1-6 alkyl optionally substituted with one of -OH, -O-C1- 6 alkyl, -O-C1-6 haloalkyl, -CN, -NO2, -N(RaRb), -C(=O) N (RaRb),-c (=O) Ra, -CO2Rc, -S(O)nRc, -SO2N(RaRb), -N(Ra)-C(Rb)=O, -N(Ra)SO2Rc, -N (Ra) SO2N(RaRb), - OC (=O) N (RaRb), -N(Ra) C (=O) N(RaRb), -O-C1-6 alkyl-C(=O) N (RaRb), - S-C1-6 alkyl-C (=O) N (RaRb),-N (Ra)-CI-6 alkyl-C (=O) N (RaRb), or -N(SO2Rc)-C1-6 alkyl-C(=O)N(RaRb), (3) -C1-6 haloalkyl, ( (-O) Ra, (5) -CO2Rc, (6)-C (=O) N (RaRb), (7) -SO2N (garb), (8) -C2-6 alkenyl, (9) -C2-6 alkenyl-C (=O)-N (Ra) 2, (10) -C2-5 alkynyl,

(11) -C2-5 alkynyl-CH2N(Ra)2, (12)-C2-5 alkynyl-CH2ORa, (13)-C2-5 alkynyl-CH2S (O) nRc, or (14) -Rk, (15)-C1 6 alkyl substituted with Rk, (16) -C1- haloalkyl substituted with Rk, (17) -C1-6 alkyl-O-Rk, (1S8)-C1-6 alkyl-O-Cl-6 alkYl-Rk, (19) -C1-6 alkyl-S(O)n-Rk, (20) -C1-6 alkyl-S(O)n-C1-6 alkyl-Rk, (21) -C1-6 alkyl-N(Ra)-Rk, (22) -C1-6 alkyl-N(Ra)-C1-6 alkyl-Rk, (23)-C1-6 alkyl-N (Ra)-Cl-6 alkyl-ORk, with the proviso that the-N (Ra)- moiety and the-ORk moiety are not both attached to the same carbon of the-Cl-6 alkyl- moiety, (24) -C1-6 alkyl-C(=O)-Rk, (25) -C1-6 alkyl-C(=O) N (Ra)-Rk, (26) -C1-6 alkyl-N (Ra) C*O)-Rk, (27) -C1-6 alkyl-C (=O) N (Ra)-C1-6 alkyl-Rk, or (28)-Cl-6 alkyl-N (Ra)-Co-6 alkyl-S (O) nRk ; wherein Rk is (i) aryl, which is optionally substituted with from 1 to 5 substituents each of which is independently -C1-6 alkyl, -C1-6 alkyl-OH, -C1-6 alkyl-O-C1-6 alkyl, -C1-6 alkyl-O-C1-6 haloalkyl, -C1-6 alkyl-N(RaRb), -C1-6 alkyl-C(=O) N (RaRb), -C1-6 alkyl-C(=O)Ra, -C1-6 alkyl-CO2Rc, -C1-6 alkyl-S (O) nRt,-O-C-6 alkyl,-C1-6 haloaIkyl,-O-CX-6 haloalkyl,-OH, halo, -N (RaRb),-C (=O) N (RaRb), -C(=O)Ra, -CO2Rc, -S(O)nRc, or -SO2N(RaRb); (ii) a 4- to 7-membered saturated or mono-unsaturated heterocyclic ring containing at least one carbon atom and from 1 to 4 heteroatoms independently selected from N, O and S, wherein the heterocyclic ring is: (a) optionally substituted with from 1 to 5 substituents each of which is independently halogen, -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo ; and

(b) optionally mono-substituted with aryl or HetA ; wherein HetA is a 5-or 6-membered heteroaromatic ring containing from X to 4 heteroatoms independently selected from N, O and S, wherein the heteroaromatic ling is optionally fused with a benzene zing, and HetA is optionally substituted with from 1 to 4 substituents each of which is independently-Cl-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo; or (iii) a 5-or 6-membered heteroaromatic ring containing from 1 to 4 heteroatoms independently selected from N, O and S, wherein the heteroaromatic ring is optionally substituted with from optionally substituted with from 1 to 4 substituents each of which is independently -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo; R5 is-H or-CI-6 alkyl ; R6 is : (1) -OH, (2) -O-C1-6 alkyl, (3) -N(RuRv), (4) -O-C1-6- haloalkyl, (5) -O-C1-6 alkyl-aryl (6) -O-C1-6 alkyl-HetB, or (7)-O-CX-6 alkyl-HetC, wherein Ru is-H or-C 1-6 alkyl, Rv independently has the same definition as Rl ; HetB is a 5-or 6-membered saturated or mono-unsaturated ring contairaing from 1 to 4 heteroatoms independently selected from N, O and S, wherein the ring is optionally substituted with from 1 to 5 substituent$ each of which is independently halogen, -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo; and HetC is a 5-or 6-membered heteroaromatic ring containing from 1 to 4 heteroatoms independently selected from N, O and S, wherein the heteroaromatic ling is optionally substituted with from 1 to 4 substituents each of which is

independently -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo ; each Ra and Rb is independently-H or-Cl-6 alkyl ; each Rc is independently a-C1-6 alkyl; and each n is independently an integer equal to 0, 1 or 2.

The present invention also includes pharmaceutical compositions containing a compound of the present invention and methods of preparing such pharmaceutical compositions.

The present invention further includes methods of treating AIDS, methods of delaying the onset of AIDS, methods of preventing AIDS, methods of preventing infection by HIV, and methods of treating infection by HIV.

Other embodiments, aspects and features of the present invention are either further described in or will be apparent from the ensuing description, examples and appended claims.

DETAILED DESCRIPTION OF THE INVENTION The present invention includes the 8-hydroxy-l-oxo-tetrahydropyrrolopyrazines of Formula (I) above. These compounds and pharmaceutically acceptable salts thereof are SV intcgrase inhibitors.

A first embodiment of the present invention is a compound of Formula (1), or a phatmaceutically acceptable salt thereof, wherein R1 is -C1-4 alkyl mono-substituted with aiyl ; wherein the aryl is optionally substituted with from 1 to 4 substituents each of which is independently (1)-C1 4 alkyl, optionally mono-substituted with -OH, -O-C1-4 alkyl, -O-C1-4 haloalkyl,-CN,-N (RaRb),-C (=O) N (RaRb),-C (-O) Ra,-C02RC,-S (O) nRc, -SO2N (RaRb),-N (Ra) C (=O) Rb, -N(Ra)CO2Rc, -N(Ra)SO2Rc, - N (Ra) S02N (RaRb),-OC (=O) N (RaRb), or-N (Ra) C (=O) N (RaRb), (2)-0-CI-4 alkyl, optionally mono-substitated with-OH,-0-CI-4 alkyl,-0-Cl-4 haloallcyl,-S (O) nRc,-N (Ra)-CO2Rc, -C(=O)N(RaRb), -SO2N(RaRb), -N (Ra) C (=O) Rb,-N (Ra) CO2Rc,-N (Ra) S02Rc,-N (Ra) S02N (RaRb), - OC (=0) N (RaRb), or-N (Ra) C (-O) N (RaRb),

(3) -C1-4 haloalkyl, (4) -O-C1-4 haloalkyl, (5) -OH, (6) halo, (7)-CN, (8) -NO2, (9) -N(RaRb), (10) -SRc, (11) -S(=O)Rc, (12) -SO2Rc, (13) -N (Ra) S02Rc, (14)-S02N (RaRb), (15) -N(ra)C(=O)Rb, or (16)-N (Ra) CO2Rc; and all other variables are as originally defined above.

An aspect of the first embodiment is a compound of Formula (I), or a pharmaceutically acceptable salt thereof, wherein RI is as defined in the first embodiment ; R6 is : (1) -OH, (2) -O-C1-6 alkyl, (3)-N (RuRV), (4) -O-C1-6 haloalkyl, (5) -O-C1- alkyl-aryl (6) -O-C1-6 alkyl-HetB, or (7) -O-c1-6 alkyl-HetC, wherein Ru is -H or -C1-6 alkyl; Rv is -H, -C1-6 alkyl, -C1-6 cycloalkyl, or independently has the same definition as R1 above;

HetB is a 5-or 6-membered saturated or mono-unsaturated ring containing from 1 to 4 heteroatoms independently selected from N, O and S, wherein the ring is optionally substituted with from 1 to 5 substituents each of which is independently halogen, -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo ; and HetC is a 5- or 6-membered heteroaromatic ring containing from l to 4 heteroatoms independently selected from N, O and S, wherein the heteroaromatic ring is optionally substituted with from 1 to 4 substituents each of which is independently -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo; and all other variables are as originally defined.

In the foregoing aspect, the reference to Rv having"the same definition as kl above"means that RI in the definition of RV is as defined in the instant embodiment, here the first embodiment, instead of as originally defined.

A second embodiment of the present invention is a compound of Formula (p, or a pharmaceutically acceptable salt thereof, wherein R1 is -(CH2)1-4-phenyl, wherein the phenyl is optionally substituted with from 1 to 3 substituents each of which is independently (1) -C1-4 alkyl, optionally mono-substituted with -OH, -O-C1-4 alkyl, -O-C1-4 haloalkyl,-CN,-N (RaRb), -C(=O)N(RaRb), -C(=O)Ra, -CO2Rc, -S(O)nRc, or -SO2N(RaRb), (2)-0-C14 alkyl, (3) -C1-4 haloalkyl, (4) -O-C1-4 haloalkyl, (5) -OH, (6) halo, <BR> <BR> <BR> (7)-CN,<BR> <BR> <BR> <BR> <BR> (8)-NO2,<BR> <BR> <BR> <BR> <BR> (9)-N (RaRb), (10)-SRc, (11)-S (=O) Rc, (12)-SO2RC,

(13) -N(Ra)SO2Rc, (14) -SO2N(RaRb), (15)-N (Ra) C (=O)Rb, or (16)-N (Ra) Co2Rc ; and all other variables are as originally defined above.

An aspect of the second embodiment is a compound of Formula (I), or a pharmaccutically acceptable salt thereof, wherein Ri is as defined in the second embodiment, R6 is as defined in the foregoing aspect of the first embodiment except that R1 in the definition of Rv is as defined in the second embodiment ; and all other variables are as originally defined.

A third embodiment of the present invention is a compound of Formula (1), or a pharmaceutically acceptable salt thereof, wherein RI is wherein S1 and X2 are each independently (1) -H, (2) methyl (3) ethyl, (4) methoxy, (5) ethoxy, (6) -CF3, (7) fluoro, (8) bromo, or (9) chloro ; and all other variables are as originally defined above.

In an aspect of the third embodiment, RI is 4-fluorobenzyl. Another aspect of the third embodiment is a compound of Formula (I), or a pharmaceutically acceptable salt thereof, wherein RI is as defined in the third embodiment, R6 is as defined in the foregoing aspect of the first embodiment except that RI in the definition of Rv is as defined in the third embodiment; and all other variables are as onginally defined. In a feature of this aspect, RI is 4-fluorobenzyl.

A fourth embodiment of the present invention is a compound ofFonnula (I), or a pharmaceutically acceptable salt thereof, wherein R2 is is-EI or-Ci-4 alkyl ; and all other variables are as originally defined or as defined in any of the preceding embodiments or aspects.

In an aspect of this embodiment, R2 is-H.

A fifth embodiment of the present invention is a compound of Formula (I), or a pharmaceutically acceptable salt thereof, wherein R3 is -H or -C1-4 alkyl ; and all other variables are as originally defined or as defined in any of the preceding embodiments or aspects thereof. In an aspect of this embodiment, R3 is-H.

A sixth embodiment of the present invention is a compound of Formula (I), or a pharmaceutically acceptable salt thereof, wherein R4 is : (1)-H, (2) -C1-4 alkyl optionally substitnted with one of -OH, -O-C1- 4 alkyl,-O-C1-4 haloalkyl,-CN,-N (RaRb),-C (=O) N (RaRb),-C (=O) Ra, -CO2Rc, -S(O)nRc, -SO2N(RaRb), -N (Ra) -C (Rb) =O,-N (Ra) S02Rb, or-N (R. a) S02N (RaRb), (3)-C (=O) N (RaRb), (4) -Rk, (5) -C1-4 alkyl substituted with Rk, (6) -C1-4 alkyl-O-Rk, or (7) -C1-4 alkyl-O-C1-4 alkyl-Rk; and all other variables are as originally defined or as defined in any of the preceding embodiments or aspects thereof.

A seventh embodiment of the present invention is a compound of Formula (1), or a pharmaceutically acceptable salt thereof, wherein R4 is : (1)-H,

(2) -C1-4 alkyl optionally substituted with one of-OR,-N (RaRb), or - C (=0) N (RaRb), (3) -C(=O)N(RaRb), (4) -(CH2)1-3-Rk, (5)- (CH2) l-3-0-Rk, or (6)- (CH2)1-3-O-(CH2)1-3Rk ; and all other variables are as originally defined or as defined in any of the first five embodiments or aspects thereof.

An eighth embodiment of the present invention is a compound of Formula (1), or a pharmaceutically acceptable salt thereof, wherein Rk is: (i) phenyl, which is optionally substituted with from 1 to 3 substituents each of which is independentiy -C1-4 alkyl, -C1-4 alkyl-OH, -C1-4 alkyl-O-C1- 4 alkyl, -C1-4 alkyl-O-C1-4 haloalkyl, -C1-4 alkyl-N(RaRb), -C1-4 alkyl-C (= N N(RaRb), -C1-4 alkyl-C(=O)Ra, -C1-4 alkyl-CO2Rc, -C1-4 alkyl-S (O) nRc, -O-C1-4 alkyl, -C1-4 haloalkyl, -O-C1-4 haloalkyl, -OH, halo, -N(RaRb), -C(=O)N(RaRb), -C(=O)Ra, -CO2Rc, -S(O)nRc, or -S02N (RaRb) ; (ii) a4- to 7-membered saturated heterocyclic zing containing at least one carbon atom and a total of from 1 to 4 heteroatoms independently selected from X to 4 N atoms, from 0 to 2 O atoms, and from 0 to 2 S atoms, wherein the heterocyclic ring is: (a) optionally substituted with from 1 to 4 substituents each of which is independently halogen, -C1-4 alkyl, -C1-4 haloalkyl, -O-C1-4 alkyl,-O-C1-4 haloalkyl, or oxo; and (b) optionally mono-substituted with phenyl or HetA ; wherein HetA is a 5-or 6-menibered heteroaromatic ring containing a total of from 1 to 4 heteroatoms independently selected from 1 to 4 N atoms, from 0 to 2 O atoms, and from 0 to 2 S atoms, wherein HetA is optionally substituted with from 1 to 3 substituents each of which is independently-C 1-4 alkyl, -C1-4 haloalkyl, -O-C1-4 alkyl,-O-C1-4 haloalkyl, or oxo ; or

(iii) a 5-or 6-membered heteroaromatic ring containing a total of from 1 to 4 heteroatoms independently selected from 1 to 4 N atoms, from 0 to 2 O atoms, and from 0 to 2 S atoms, wherein the heteroaromatic zing is optionally substituted with from optionally substituted with from 1 to 3 substituents each of which is independently-C1 alky],-Cl-6 haloalkyi, -O-C1-6 alkyl, -O-C1-6 haloalkyl, or oxo ; and all other variables are as originally defined or as defined in any of the preceding embodiments or aspects thereof.

In an aspect of the eighth embodiment, HetA is a 5-or 6-membered heteroaromatic ring containing 1 or 2 N atoms, wherein HetA is optionally substituted with from 1 to 3 substituents each of which is independently-C1-4 alkyl, -C1-4 haloalkyl, -O-C1-4 alkyl, -O-C1-4 haloalkyl, or oxo. In another aspect of the eighth embodiment, HetA is pyrrolyl, pyrazolyl, imidazolyl, pyridyl, or pyrazinyl ; which is optionally substituted with from 1 to 3 substituents each of which is independently-C 1-4 alkyl (e. g., rnethyl),-C1g haloalkyl (e. g., trifluoromethyl), -O-C1-4 alkyl (e.g., methoxy), -O-C1-4 haloalkyl (e. g.,-OCF3), or oxo.

A ninth embodiment of the present invention is a compound of Formula (I), or a pharmaceutically acceptable salt thereof, wherein Rk is : (i) phenyl, which is optionally substituted with from 1 to 3 substituents each of which is independently-Cl-4 alkyl, -C1-4 alkyl-OH, -C1-4 alkyl-O-C1- 4 alkyl, -C1-4 alkyl-O-C1-4 haloalkyl, -C1-4 alkyl-N(RaRb), -C1-4 alkyl-C (=O) N (RaRb), -C1-4 alkyl-C(=O)Ra, -C1-4 alkyl-CO2Rc, -C1-4 alkyl-S (O) nRc, -O-C1-4 alkyl, -C1-4 haloalkyl, -O-C1-4 haloalkyl, -OH, halo, -N (RaRb),-C (=O) N (RaRb),-C (=O) Ra, -CO2Rc, -S (O) nRc, or -SO2N(RaRb)p or (ii) a saturated heterocyclic ring selected from the group consisting of piperidinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isooxazolidinyl, pyrrolidinyl, imidazolidinyl, piperazinyl, tetrahydrofuranyl, tetrahydrothienyl, pyrazolidinyl, hexahydropyrimidinyl, thiazinanyl, thiazepanyl, thiadiazepanyl, dithiazepanyl, azepanyl, diazepanyl, thiadiazinanyl, and dioxanyl ; wherein the saturated heterocyclic ring is :

(a) optionally substituted with from 1 to 4 substituents each of which is independently halogen, -C1- 4 alkyl, -C1-4 haloalkyl, -O-C1-4 alky],-O-Cl-4 haloalkyi, or oxo ; and (b) optionally mono-substituted with phenyl or HetA ; wherein HetA is a heteroaromatic ring selected from the group consisting of pyridyl, pyrrolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, thienyl, furanyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isooxazolyl, oxadiazolyl, oxatriazolyl, thiazolyl, isothiazolyl, and thiadiazolyl ; wherein the heteroaromatic ring is optionally substituted with from 1 to 3 substituents each of which is independently-C14 alkyl, -C1-4 haloalkyl, -O-C1-4 alkyl, -O-C1- 4 haloalkyl, or oxo ; and all other variables are as originally defined or as defined in any of the first seven embodiments or aspects thereof- A tenth embodiment of the present invention is a compound of Formula (I), or a pharmaceutically acceptable salt thereof, wherein RS is-XI ; and all other variables are as originally defined or as defined in any of the preceding embodiments or aspects thereof.

An eleventh embodiment of the present invention is a compound of Formula (I), or a pharmaceutically acceptable salt thereof, wherein R6 is : (1) -OH, (2)-0-C14 alkyl, (3)-N (RuRv),<BR> (4)-0-C14 haloalkyl, (5) -O-C1-4 alkyl-aryl (6) -O-C1-4 alkyl-HetB, or (7) -O-C1-4 alkyl-HetC, wherein Ru is-or-C1 4 alkyl ; Rv is -H, -C1-4 alkyl, or cyclopropyl ; HetB is a 5-or 6-membercd saturated ring containing a total of from 1 to 4 heteroatoms independently selected from 1 to 4 N atoms, from 0 to 2 O atoms,

and from 0 to 2 S atoms, wherein the saturated ring is optionally substituted with from 1 to 4 substituents each of which is independently halogen,-C1 4 alkyl, - Cl-4 haloalkyl,-O-CX-4 alkyl,-O-CC-4 haloalkyl, or oxo ; and HetC is a 5-or 6-memebered heteroaromatic ring containing a total of from I to 4 heteroatoms independently selected from 1 to 4 N atoms, from 0 to 2 O atoms, and from 0 to 2 S atoms, wherein the heteroaromatic ring is optionally substituted with from 1 to 3 substituents each of which is independently-Cl-4 alkyl, -C1-4 haloalkyl, -O-C1-4 alkyl, -O-C1-4 haloalkyl, or oxo; and all other variables are as originally defined or as defined in any of the first ten embodiments or aspects thereof A twelfth embodiment of the present invention is a compound of Formula (I), or a pharmaceutically acceptable salt thereof, wherein R6 is : <BR> <BR> <BR> (1)-OH,<BR> <BR> <BR> <BR> <BR> (2)-0-CI-4 alkyl,<BR> <BR> <BR> <BR> <BR> <BR> <BR> (3)-N (RU)), (4) -O-C1-4 haloalkyl, or (5) -O-C1-4 alkyl-HetC, wherein Ru is-H or-C14 alkyl ; Rv is -C1-4 alkyl or cyclopropyl ; HetC is a 5-or 6-membered heteroaromatic ring containing a total of from 1 to 4 heteroatoms independently selected from 1 to 4 N atoms, Rom 0 to 2 O atoms, and from 0 to 2 S atoms, wherein the ling is attached to the remainder of the compound via a ring carbon atom and a ring N atom is alpha to the ring carbon attached to the remainder of the compound; and wherein the heteroaromatic ring is optionally substituted with from 1 to 3 substituents each of which is independently -C1- 4 alkyl, -C1-4 haloalkyl, -O-C1-4 alkyl, -O-C1-4 haloalkyl, or oxo ; and all other variables are as originally defined or as defined in any of the first ten embodiments or aspects thereof.

A first class of the present invention includes compounds of Formula (II), or a pharmaceutically acceptable salt thereof :

wherein : wherein X1' and X2' are each independently: (1) -H, (2) C1-4 alkyl, (2) -O-C1-4 alkyl, (3) -C1-4 haloalkyl, (4)-O-Cl-4 haloalkyl, or (5) halo ; and R6'is : (1)-OH, (2) -O-C1- alkyl, or (3)-N (RuRv); wherein Ru is -H or -C1-4 alkyl ; and Rv is-Cl-4 alkyl or cyclopropyl.

A sub-class of the first class includes compounds of Formula (II), or a pharmaceutically acceptable salt thereof, wherein : Xl'and X2'are each independently : (1)-H, (2) methyl, (2)-OCH3, (3) -CF3,

(4)-OCP3, chloro, (6) Suoro, or (7) bromo ; and R6'is : (1) -OH, (2) methoxy (3) ethoxy (4) -N(RuRv); wherein Ru is-H ; and Rv is methyl, ethyl, or cyclopropyl.

A second class of the present invention includes compounds of Formula (III), or a phamaceutically acceptable salt thereof : wherein Xl and X2'are each independently-H or halo ; and R6' is: (1) -OH, (2) -O-C1-4 alkyl, or (3) -N(RuRv); wherein Ru is -H or -C1-4 alkyl ; and Rv is-C1 4 alkyl or cyclopropyl.

A sub-class of the second class includes compounds of Formula (III), or a pharmaceutically acceptable salt thereof, wherein :

X1' and X2' are each independently-H, fluoro, chloro, or bromo ; and R6' is: (1) -OH, (2) methoxy (3) ethoxy (4)-N (RURV) ; wherein Ru is-H ; and Rv is methyl, ethyl, or cyclopropyl.

A thirteenth embodiment of the present invention is a. compound of Formula (IV), or a pharmaceutically acceptable salt thereof : wherein Ru is -H or -C1-6 alkyl ; Rv is C1-6 alkyl which is substituted with 1 or 2 substituents each of which is independently : (1) C3-8 cycloalkyl, (2) aryl, (3) a 5- or 6-membered saturated or mono-unsaturated heterocyclic ring containing from I to 4 heteroatoms independently selected from N, O and S, (4) a 5-or 6-membered heteroaromatic ring containing from 1 to 4 heteroatoms independently selected from N, O and S, or (5) a 9-or 10-nlembered fused bicyclic heterocycle containing from I to 4 heteroatoms independently selected from N, O and S, wherein at least one of the rings is aromatic; wherein

(A) each cycloalkyl is optionally substituted with from 1 to 3 substituents, each of which is independently halo,-Cr-6 alkyl, or-O-C1-6 alkyl ; (B) each aryl is optionally substituted with from 1 to 5 substituents each of which is independently (1) -C1- alkyl, optionally substituted with from 1 to 3 substituents each of which is independently-OH,-O-Cl-6 alkyl,-O-Ci-6 haloalkyl,-CN,-N02,-N (RaRb), -C (=O) N (RaRb),-C (=O) Ra, -CO2Rc, -S(O)nRc, -SO2N(RaRb), -N (Ra) C (=O) Rb, -N(Ra)CO2Rc, -N(Ra)SO2Rc, -N(Ra) S02N (RaRb), - OC (=0) N (RaRb), or-N (Ra) C (=O) N (RaRb), (2) -O-C1-6 alkyl, optionally substituted with from 1 to 3 substituents each of which is independently -OH, -O-C1-6 alkyl, -O-C1-6 haloalkyi,-S (0) nRc,-C (=0) N (RaRb),-S02N (RaRb), -N(Ra)C(=O)Rb, -N(Ra)CO2Rc, -N (Ra) SO2Rc, -N(Ra)SO2N(RaRb), -OC(=O)N(RaRb), or -N(Ra)C(=O) N (RaRb), (3)-Cl-6 haloalkyl, (4) -O-C1-6 haloalkyl, (5) -OH, (6) halo, (7)-CN, (8) -NO2, (9)-N N-(RaRb), (10) -C(=O) N (RaRb), <BR> <BR> <BR> (11)-C (=O) Ra,<BR> <BR> <BR> (12)-CO2Rc,<BR> <BR> <BR> <BR> <BR> (13)-SRc, (14) -S (=O)Rc, (15) -SO2Rc, (16) -N(Ra)SO2Rc, (17)-S02N (RaRb), (18) -N (Ra) C (=O) Rb, or (19) -N(Ra)CO2Rc; (C) each saturated or mono-unsaturated heterocyclic ring is

(i) optionally substituted with from 1 to 5 substituents each of which is independently halogen, -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl,-O-C1-6 haloalkyl, or oxo; and (ii) optionally substituted with 1 or 2 substituents each of which is independently aryl or a 5-or 6-membered heteroaromatic ring containing from 1 to 4 heteroatoms independently selected from N, O and S ; and (D) each heteroaromatic ring or each fused bicyclic heterocycle is (i) optionally substituted with from 1 to 7 substituents each of which is independently halogen, -C1-6 alkyl, -C1-6 haloalkyl, -O-C1-6 alkyl,-O-Cl-6 haloalkyl, or oxo; and (ii) optionally substituted with 1 or 2 substituents each of which is independently aryl, or -C alkyl-aryl ; Ri is-H or-C1 6 alkyl ; and all other variables are as originally defined above or as defined in any of the previous embodiments or aspects thereof.

A fourteenth embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein Rv is -C1-4 alkyl mono-substituted with aryl ; wherein the aryl is optionally substituted with from I to 4 substituents each of which is independently (1) -C1-4 alkyl, optionally mono-substituted with -OH, -O-C1-4 alkyl, -O-C1-4 haloallyl,-CN,-N (RaRb),-C (=O) N (RaRb),-C (=O) Ra,-C02RC,-S (O) nRc, -SO2N (RaRb),-N (Ra) C (=O) Rb, -N (Ra) CO2Rc,-N (Ra) S02RC, - N (Ra) SO2N (RaRb), -OC(=O) N (RaRb), or-N (Ra) C (=O) N (RaRb), (2) -O-C1-4 alkyl, optionally mono-substituted with -OH, -O-C1-4 alkyl, -O-C1-4 haloalkyl, -S(O)nRc, -N(Ra)-CO2Rc, -C(=O) N (RaRb), -SO2N(RaRb), -N(Ra) C (=O) Rb, -N (Ra) CO2Rc,-N (Ra)SO2Rc, -N(Ra)SO2N(RaRb), -OC(=O)N(RaRb), or-N(Ra) C (=O) N (RaRb), (3) -C1-4 haloalkyl, ($) -O-C1-4 haloalkyl, (5)-OH,

(6) halo, (7)-CN, (8) -NO2, (9) -N (RaRb), (10) -SRc, (11)-S (=O) Rc (12) -SO2Rc, (13) -N (Ra) S02Rc, (14) -SO2N(RaRb), (15) -N(Ra) C (=O) Rb, or (16)-N (Ra) C02RC ; and all other variables are as first defined above for Formula (IV).

A fifteenth embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein Rv is -(CH2)1-4-phenyl, wherein the phenyl is optionally substituted with from 1 to 3 substituents each of which is independently (1) -C1-4 alkyl, optionally mon-substituted with -OH, -O-C1-4 alkyl, -O-C1-4 haloalkyl, -CN, -N (RaRb), -C (=O) N (RaRb), -C(=O)Ra, -CO2Rc, -S(O)nRc, or -SO2N(RaRb), (2) -O-C1-4 alkyl, (3) -C1-4 haloalkyl, (4) -O-C1-4 haloalkyl, (5)-OH, (6) halo, (7)-CN, (8) -NO2, (9)-N -N(RaRb), (10) -SRc, (11) -S (=O) Rc, (12)-S02RC, (13) -N (Ra) SO2Rc, (14)-S02N (RaRb), (15)-N (Ra) C (z--O) Rb, or

(16) -N (Ra) ; and all other variables are as first defined above in Founula (IV).

A sixteenth embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein Rv is : X1 and X are each independently (1)-H, (2) methyl, (3) ethyl, (4) methoxy, (5) ethoxy, (6)-CF3, (7) fluoro, (8) bromo, or (9) chloro ; and all other variables are as first defined above in Formula In an aspect of the sixteenth embodiment, Rv is 4-fluorobenzyl.

A seventeenth embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein RU is-H ; and all other variables are as first defined in Forumla (IV) or as defined in the preceding embodiments or aspects thereof.

An eighteenth embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein R5 is -H, and all other variables are as first defined in Formula (IV) or as defined in the preceding embodiments or aspects thereof.

A nineteenth embodiment of the present invention is a compound of Formula (IV), or a pharrnaceuticaIly acceptable salt thereof, wherein R4 is: (1) -H, (2) -C1-4 alkyl optionally substituted with one of -OH, -N(RaRb), or -C (=O) N (RaRb), (3)-C (=O) N (RaRb), (4) -(CH2)1-3-Rk, (5) -(CH2)1-3-O-Rk, or (6) -(CH2)1-3-O(CH2)1-3-Rk; and all other variables are as first defined in Formula (IV) or as defined in the preceding embodiments or aspects thereof.

A twentieth embodiment of the present invention is a compound of Formula (IV), or a pharraaaceutically acceptable salt thereof, wherein Rk is the same as defined above in the eighth embodiment for compounds of Formula (1) ; and all other variables are as originally defined in Formula (IV) or as defined in any of the preceding embodiments or aspects thereof.

In an aspect of the twentieth embodiment, HetA is a 5-or 6-membered heteroaromatic ring containing 1 or 2 N atoms, wherein HetA is optionally substituted with from 1 to 3 substituents each of which is independently -C1-4 alkyl, -C1-4haloalkyl, -O--C1-4 alkyl, -O-C1-4 haloalkyl, or oxo. In another aspect of the eighth embodiment, HetA is pyrrolyl, pyrazolyl, imidazolyl, pyridyl, or pyrazinyl ; which is optionally substituted with from 1 to 3 substituents each of which is independently -C1-4 alkyl (e. g., methyl), -C1-4 haloalkyl (c. g. , trifluoromethyl),-O-C1-4 alkyl (e. g., methoxy),-O-CIX haloalkyl (e. g.,-OCF3), or oxo.

A twenty first embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein Rk is the same as defined above in the ninth embodiment for compounds of Formula (1) ; and all other variables are as originally defined in Formula (IV) or a$ defined in any of the preceding embodiments or aspects thereof.

A twenty-second embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein R2 is-H ; and all other variables are as originally defined or as defined in the preceding embodiments or aspects thereof.

A twenty third embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein kl is-C1-4 alkyl ; and all other variables are as originally defined or as defined in the preceding embodiments or aspects thereof.

In an aspect of this embodiment, R1 is methyl or ethyl- A twenty fourth embodiment of the present invention is a compound of Formula (IV), or a pharmaceutically acceptable salt thereof, wherein RI is-H, methyl, or ethyl. ; and all other variables are as originally defined or as defined in the preceding embodiments or aspects thereof.

A twenty-fifth embodiment of the present invention is a compound selected from the group consisting of : ethyl 2-benzyl-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a] pyrazine-7-carboxylate ; 2-benzyl-8-hydroxy-1-ox-1, 2,3, 4-tetrahydropyrrolo[1,2-a]pyrazine-7-carboxylic acid; ethyl 2- (4-fluorobenzyl)-8-hydroxy-l-oxo-1, 2,3, 4-tetrahydropyrrolo [1,2-a]pyrazine-7- carboxylate ; 2- (4-fluorobenzyl)-8-hydroxy-l-oxo-1, 2, 3,4-tetrahydropyrrolo[1,2-a]pyrazine-7-carboxylic acid; 2-(4-fluorobenzyl)-8-hydroxy-N-methyl-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a] pyrazine-7- carboxamide ; 2- (4-fluorobenzyl)-8-hydroxy-N-ethyl-1-oxo-1, 2, 3,4-tetrahydropyrrolo[1,2-a]pyrazine-7- carboxamide; 2-(4-fluorobenzyl)-8-hydroxy-N-cyclopropyl-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a]pyrazine-7- carboxamide;

ethyl 2-(3-chlorobenzyl)-8-hydroxy-1-oxo-1,2,3,4-tetrahydropyrrolo [1,2-α]pyrazine-7- carboxylate ; 2-(3-chlorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo[1,2-α]pyrazine-7-carboxylic acid; N- (4-fIuorobenzyl)-8-hydroxy-2-methyl-1-oxo-1, 2,3, 4-tetrahydropyrrolo[1, 2-a] pyrazine-7- carboxaroide ; ethyl 2- (3-fluorobenzyl)-S-hydroxy-l-oxo-3., 2, 3, 4-tetrahydropyrrolo [1, 2-α]pyrazine-7- carboxylate ; ethyl 2-(3,4-fluorobenzyl)-8-hydroxy-1-oxo-1,2, 3, 4-tetrahydropyrrolo [1, 2-α]pyrazine-7- carboxylate ; ethyl 2-(4-chlorobenzyl)-8-hydroxy-1-oxo-1,2, 3, 4-tetrahydropyrrolo[1,2-α]pyrazine-7- carboxylate ; ethyl 2-(3-chloro-4-fluorobenzyl)-8-hydroxy-1-oxo-1,2, 3, 4-tetrahydropyrrolo[1,2-α]pyrazine-7- carboxylate; ethyl 2-(3,5-dichlorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-ajpyrazine-7- carboxylate ; 2-(4-chlorobenzyl)-8-hydroxy-N-methyl-1-oxo-1, 2, 3,4-tetrahydropyrrolo[1,2-α]pyrazine-7- carboxamide ; 2- (3,4-difluorobenzyl)-8-hydroxy-N-methyl-1-oxo-1,2, 3, 4-tetrahydropyrrolo [1, 2-a] pyrazine-7- carboxamide ; 2-(3,4-dichlorobenzyl)-8-hydroxy-N-methyl-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a] pyrazine-7- carboxamide; and pharmaceutically acceptable salts thereof.

Other embodiments of the present invention include compounds of Formula (I) and (IV) respectively, wherein each Ra and Ab is independently-H or-CI-4 alkyl ; each Rc is independently a-CI-4 alkyl ; and all other variables are as originally defined or as defined in any of the foregoing embodiments or aspects thereof.

Still other embodiments of the present invention include compounds of Formula (I) and (IV) respectively, wherein each Ra and Rb is independently-H, methyl, or ethyl; each Rc is independently methyl or ethyl, and all other variables are as originally defined or as defined in any of the foregoing embodiments or aspects thereof.

Other embodiments of the present invention include the following : (a) A pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention (e. g. , a compound of Formula (1) or Formula (II) or Formula (HI) or Formula (IV) or any of the specific compounds set forth above) and a pharmaceutically acceptable carrier.

(b) A pharmaceutical composition which comprises the product prepared by combining (e. g., mixing) a therapeutically effective amount of a compound of the invention and a phaxmaccuUcally acceptable carrier- (c) The pharmaceutical composition of (a) or (b), further comprising a therapeutically effective amount of an HIV infection/AIDS treatment agent selected from the group consisting of V/AIDS antiviral agents, immunomodulators, and anti-infective agents.

(d) The pharmaceutical composition of (c), wherein the SV infection/AIDS treatment agent is an antiviral selected from the group consisting of HW protease inhibitors, non- nucleoside HTV reverse transcriptase inhibitors, and nucleoside HIV reverse transcriptase inhibitors.

(e) A combination useful for inhibiting EV integrase, for treating or preventing infection by HIV, or for preventing, treating or delaying the onset of AIDS, which is a therapeutically effective amount of a compound of the invention and a therapeutically effective amount of an pIIV'infection/AIDS treatment agent selected from the group consisting of HIV/AIDS antiviral agents, immunomodulators, and anti-infective agents.

(f) The combination of (e), wherein the HIV infection/AIDS treatment agent is an antiviral selected from the group consisting of H protcase inhibitors, non-nucleoside HIV reverse transcriptase inhibitors and nucteoside HTV reverse transcriptase inhibitors.

(method of inhibiting HIV integrase in a subject in need thereof which comprises administering to the subject a therapeutically effective amount of a compound of the invention.

(h) A method of preventing or treating infection by HIV in a subject in need thereof which comprises administering to the subject a therapeutically effective amount of a compound of the invention.

(i) The method of (h), wherein the compound of the invention is administered in combination with a therapeutically effective amount of at least one antiviral selected-from the group consisting of IiIV protease inhibitors, non-nucleoside HIY reverse transcriptase inhibitors, and nucleoside HIV reverse transcriptase inhibitors.

(j) A method of preventing, treating or delaying the onset of AIDS in a subject in need thereof which comprises administering to the subject a therapeutically effective amount of a compound of the invention.

(k) The method of (j), wherein the compound is administered in combination with a therapeutically effective amount of at least one antiviral selected from the group consisting of HIV protease inhibitors, non-nucleoside HIV reverse transcriptase inhibitors, and nucleoside S1 reverse tanscIiptase inhibitors (1) A method of inhibiting HIV integrase in a subject in need thereof which comprises administering to the subject the pharmaceutical composition of (a), (b), (c) or (d) or the combination of (e) or (f).

(m) A method of preventing or treating infection by UN in a subject in need thereof which comprises administering to the subject the pharmaceutical composition of (a), (b), (c) or (d) or the combination of (e) or (f).

(n) A method of preventing, treating or delaying the onset of AIDS in a subject in need thereof which comprises administering to the subject the pharmaceutical composition of (a), (b), (c) or (d) or the combination of (e) or (f) The present invention also includes a compound of the present invention (i) for use in, (ii) for use as a medicament for, or (iii) for use in the preparation of a medicament for: (a) inhibiting SI protease, (b) preventing or treating infection by HIV, or (c) preventing, treating or delaying the onset of AIDS In these uses, the compounds of the present invention can optionally be employed in combination with one or more HIV/AIDS treatment agents selected from HIV/AIDS antiviral agents, anti-infective agents, and immunomodulators.

Additional embodiments of the invention include the pharmaceutical compositions, combinations and methods set forth in (a)- (n) above and the uses set forth in the

preceding paragraph, wherein the compound of the present invention employed therein is a compound of one of the embodiments, or an aspect or feature or sub-feature thereof, described above.

In all of the foregoing embodiments describing compositions, combinations and methods, the compound may optionally be used in the form of a pharmaceutically acceptable salt.

As used herein, the term"C1 ó alkyl" (or'; C1-C6 alkyl") means a linear or branched chain alkyl group having from 1 to 6 carbon atoms and includes all of the hexyl alkyl and pentyl alkyl isomers as well as n-, iso-, sec-and t-butyl, n-and isopropyl, ethyl and methyl.

"C1-4 alkyl" means n-, iso-, sec-and t-butyl, n-and isopropyl, ethyl and methyl.

The term"Co"as employed in expressions such as "-C0-6 alkyl-" means a direct covalent bond. For example, in the group-Cl-6 alkyl-N (Ra)-Co-6 alkyl-S (O) nRk, when the second alkylen group is"Co", then the group is-C1 6 alkyl-N (Ra)-s (o) nRk The term"-C1-6 alkyl-"refers to a Cl to C6 linear or branched al group as just defined which is bivalent. It can alternatively be referred to as"Ci-g alkylene"or"Cl-6 alkanediyl". A class of alkylenes of particular interest with respect to the invention is- (CH2) 1 6-, and sub-classes of particular interest include- (CH2) 1-4-, -(CH2)1-3-, -(CH2)1-2-, and -CH2-.

The term"C2-6 alkenyl" (or"C2-C6 alkenyl") means a linear or branched chain alkenyl group having from 2 to 6 carbon atoms and includes all of the hexenyl and pentenyl isomers as well as 1-butenyl, 2-butenyl, 3-butenyl, isobutenyl, 1-propenyl, 2-propenyl, and ethenyl (or vinyl). Similar terms such as"C2-4 alkenyl"have an analogous meaning. A class of alkenyls of particular interest with respect to the invention is-CH2=CH-(CH2)0-4H, and sub- classes of particular interest include-CH=CH- (CH2) 1-2H,-CH=CH-CH3, and-CH=CH2.

Another class of alkenyls of particular interest with respect to the invention is alkenyls selected from -(CH2)2-CH=CH-(CH2)0-2H and -CH2-CH=CH-(CH2)0-3H.

The term"C2-5 alkynyl" (or"C2-C5 alkynyl") means a linear or branched chain alkynyl group having from 2 to 5 carbon atoms and includes all of the pentynyl isomers as well as 1-butynyl, 2-butynyl, 3-butynyl, 1-propynyl, 2-propynyl, and ethynyl (or acetylenyl). Similar tenns such as"C2-4 alkynyl"have an analogous meaning. A class of alkynyls of particular interest with respect to the invention is (CH2)1-4H (e.g., -C#C-CH3). Another class of alkynyls of particular interest with respect to the invention is alkynyls selected from -CH2#(CH2)1-3H and -(CH2)2#(CH2)1-2H.

The term"C3-g cycloalkyl" (or"C3-Cg cycloalkyl") means a cyclic ring of an alkane having three to eight total carbon atoms (i. e., cyclopropyl, cyclobutyl, cyclopentyl,

cyclohexyl, cycloheptyl, or cyclooctyl). Similar terms such as "C3-6 cycloalkyl" have an analogous meaning.

The term"halogen" (or"halo") refers to fluorine, chlorine, bromine and iodine (alternatively referred to as fluoro, chloro, bromo, and iodo).

The term"C1-6 haloalkyl" (which may alternatively be referred to as"C1-C6 haloalkyl"or"halogenated Cf-C6 alkyl") means a Cl to C6 linear or branched alkyl group as defined above with one or more halogen substituents. The term"C1-4 haloalkyl"has an analogous meaning. The term"Cl-6 fluoroalkyl"has an analogous meaning except that the halogen substituents are restricted to fluoro. A class of fluoroalkyls of particular interest with respect to the invention is the seiies (CH2) 0-4CF3 (i. e., trifluoromethyl, 2, 22-trifluoroethyl, 3,3, 3-trifluoro-n-propyl, etc.).

The term"oxo"means a divalent oxygen substituent ; i. e., =O. An oxo substituent on a carbon atom in a heteroaromatic ring refers to the keto form of the keto-enol tautomer, as exemplified here for an oxopyridinyl substituent : Compounds of the present invention having an oxo substituent on a carbon atom of a heteroaromatic ring are understood to include compounds in which only the keto form is present, compounds in which only the enol form is present, and compounds in which the keto and enol forms are both present.

The term"aryl"as used herein refers to an aromatic carbocyclic ring or an aromatic carbocyclic fused ring system. The fused zing system contains two or more carbocyclic rings in which each ling shares two adjacent carbon atoms with at least one other ring. The aryl group may be attached to the rest of the molecule at any carbon atom which results in a stable compound A subset of aryl groups particularly suitable for use in the present invention (e. g. , in the definition of Rk) includes those selected from phenyl, naphthyl, anthryl, and phenanthryl.

Another particularly suitable subset of aryl groups is phenyl and naphthyl. Still another particularly suitable subset of aryl groups is phenyl per se.

The term"hereroeyclic ring"refers to a 4-to 8-membered, saturated or unsaturated monocyclic ring that contains one or more heteroatoms (e. g., from 1 to 6

heteroatoms, from 1 to 5 heteroatoms, from 1 to 4 heteroatoms, from 1 to 3 heteroatoms, 1 or 2 heteroatoms, or 1 heteroatom) independently selected from N, O and S and a balance of carbon atoms (the ring typically contains at least one carbon atom) ; and wherein any one or more of the nitrogen and sulfur heteroatoms is optionally oxidized, and any one or more of the nitrogen heteroatoms is optionally quaternized. The heterocyclic ring may be attached to the rest of the molecule via any heteroatom or carbon atom in the ring, provided that attachment results in the creation of a stable structure. When the heterocyclic ring has substituents, it is understood that the substituents may be attached to any atom in the nng, whether a heteroatom or a carbon atom, provided that a stable chemical structure results.

A subset of the heterocyclic rings useful in the present invention (e. g., in the definition of) includes any 4-to 7-membered saturated or mono-unsaturated heterocyclic ring, wherein the ring contains at least one carbon atom and from 1 to 4 heteroatoms independently selected from N, O and S A subgroup of this subset includes any 4-to 7-membered saturated or mono-unsaturated heterocyclic ring in which the ring contains at least one carbon atom and a total of from 1 to 4 heteroatoms independently selected from 1 to 4 N atoms, from 0 to 2 O atoms, and from 0 to 2 S atoms. Representative examples of saturated heterocyclic rings include piperidinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isooxazolidinyl, pyIrolidínyl, ilIiidazolidinyl, piperazinyl, tetrahydrofuranyl, tetrahydrothienyl, pyrazolidinyl, hexahydropyrimidinyl, thiazinanyl (e. g., 1, 2-thiazinanyl C), thiazepanyl7 tliiadiazepanyl dithiazepanyl, azepanyl (i. e., N'', diazepanyl, thiadiazinanyl (e. g., 1, 2, 6- N thiadiazinanyl N), and dioxanyl, Representative examples of mono-unsaturated rings are the same as the saturated rings listed in the preceding sentence except that each ring contains a double bond.

Another subset of the heterocyclic rings useful in the present invention (e. g. , in the definition of HerB) includes any 5-or 6-membered saturated or mono-unsaturated ring containing from 1 to 4 heteroatoms independently selected from N, O and S A useful subgroup of this subset includes any 5-or 6-membered saturated or mono-unsaturated heterocyclic ring in which the ring contains at least one carbon atom and a total of from 1 to 4 heteroatoms independently selected from I to 4 N atoms, from 0 to 2 0 atoms, and from 0 to 2 S atoms.

Another useful subgroup is identical to the preceding subgroup, except that it is limited to saturated heterocyclic rings. Still another subgroup of this subset of heterocyclic rings suitable

for use in the present invention includes any 5-or 6-membered saturated ring containing 1 or 2 N atoms and carbon atoms. Representative examples of this subgroup include piperidinyl, pyrazolidinyl, imidazolidinyl, piperazinyl, piperidinyl, andhexahydropyrimidinyl.

Still another subset of the heterocyclic rings useful in the present invention are the heteroaromatic rings-The term"heteroaromatic ring" (alternatively"heteroaryl ring") generally refers to a heterocyclic ring as defined above in which the nng is an aromatic ring. A useful subgroup of this subset (e. g. , in the definition of Rk, HetA, or HetC) includes any 5-or 6- membered monocyclic aromatic ring which consist of carbon atoms and from 1 to 4 heteroatoms independently selected from N, O and S. Representative examples of this subgroup include pyridyl, pyrrolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, thienyl (or thiophenyl), thiazolyl, furanyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isooxazolyl, oxadiazolyl, oxatriazolyl, thiazolyl, isothiazolyl, and thiadiazolyl-Another useful subgroup of this subset includes any 5-or 6-membered heteroaromatic ring in which the ring contains a total of from I to 4 heteroatoms independently selected from I to 4 N atoms, from 0 to 2 O atoms, and from 0 to 2 S atoms. Another useful subgroup includes any 5-or 6-membered heteroaromatic ring containing 1 or 2 N atoms and carbon atoms- The term"fused bicyclic heterocycle"refers to any 8-to 12-membered bicyclic ring system containing one or more heteroatoms (e g. , from 1 to 6 heteroatoms, from 1 to 5 heteroatoms, from 1 to 4 heteroatoms. from 1 to 3 heteroatoms, 1 or 2 heteroatoms, or 1 heteroatom) independently selected from N, O and S, in which one ring contains all of the heteroatoms or each ring contains at least one of the heteroatoms, and wherein each ring is saturated or unsaturated, and two adjacent ring atoms are shared by each of the rings in the ring system and each of the two shared atoms is independently a carbon atom or a heteroatom Any one or more of the nitrogen and sulfur heteroatoms in the ling system is optionally oxidized, and any one or more of the nitrogen heteroatoms is optionally quaternized. The fused bicyclic heterocycle may be attached to the rest of the molecule via any heteroatom or carbon atom in the ring, provided that attachment-results in the creation of a stable structure. When the bicyclic heterocycle has substituents, it is understood that the substituents may be attached to any atom in the ring, whether a heteroatom or a carbon atom, provided that a stable chemical structure results.

A subset of the fused bicyclic heterocycles useful in the present invention (e. g., in the definition of R1) includes any 9-or 10-membered fused bicyclic heterocycle containing from 1 to 4 heteroatoms independently selected from N, O and S, wherein at least one of the rings is aromatic. Representative examples of bicyclic heterocycles in this subset include benzotriaxolyl, indolyl, isoindolyl, indazolyl, indolinyl, isoindolinyl, quinoxalinyl, quinazolinyl, cinnolinyl, chromanyl, isochromanyl, tetrahydroquinolinyl, quinolinyl, ter ydroisoquinolinyl, . 0 isoquinolinyl, 2, 3-dihydrobenzofuranyl, 2, 3-dihydrobenzo-174-dioxinyl (i. e, °) and benzo-1, 3 dioxolyl (i. e., C o)

Unless expressly stated to the contrary, an"unsaturated"ring is a partially or fully unsaturated ring.

Unless expressly stated to the contrary, all ranges cited herein are inclusive. Por example, a heterocyclic ring described as containing from"1 to 4 heteroatoms"means the ring can contain 1,2, 3 or 4 heteroatoms.

When any variable (e. g., Ra, Rb, or Rc) occurs more than one time in any constituent or in Formula (1) or in any other formula depicting and describing compounds of the invention, its definition on each occurrence is independent of its definition at every other occurrence. Also, combinations of substituents and/or variables are permissible only if such combinations result in stable compounds.

The term"substituted" (e.g., as in"each aryl is optionally substituted with from 1 to 5 substituents...") includes mono-and poly-substitution by a named substituent to the extent such single and multiple substitution (including multiple substitution at the same site) is chemically allowed- iThe symbol"w"in front of an open bond in the structural formula of a group marks the point of attachment of the group to the rest of the molecule.

The compounds of the present invention may have asymmetric centers and may occur, except when specifically noted, as mixtures of stereoiaomers or as individual diastereomers, or enantiomers, with all isomeric forms being included in the present invention As would be recognized by one of ordinary skill in the art, all of the compounds of the present invention can exist as tautomers such as the following:

It is to be understood for the purposes of the present invention that a reference herein to a compound of Formula (1) is a reference to compound I per se, or to any one of its tautomers per se (e. g, IA orIB), or to mixtures of two or more of the tautomers (e. g. , two or more of I, IA, and IB).

The compounds of the present invention are useful in the inhibition of HIV integrase, the prevention or treatment of infection by hunlan immunode. ficiency virus (g) and the prevention, treatment or the delay in the. onset of consequent pathological conditions such as AIDS. Preventing AIDS, treating AIDS, delaying the onset of AIDS, or preventing or treating infection by HIV is defined as including, but not limited to, treatment of a wide range of states of EV infection : AIDS, ARC (AIDS related complex), both symptomatic and asymptomatic, and actual or potential exposure to HIV. For example, the compounds of this invention are useful in treating infection by HIV after suspected past exposure to EI1V by such means as blood transfusion, exchange of body fluids, bites, accidental needle stick, or exposure to patient blood during surgery.

The compounds of this invention are useful in the preparation and execution of screening assays for antiviral compounds. For example, the compounds of this invention are useful for isolating enzyme mutants, which are excellent screening tools for more powerful antiviral compounds. Furthermore, the compounds of this invention are useful in establishing or determining the binding site of other antivirals to HIV integrase, e. g., by competitive inhibition.

Thus the compounds of this invention are commercial products to be sold for these purposes.

The compounds of the present invention can be administered in the form of pharmaceutically acceptable salts. The term"pharmaceutically acceptable salt"refers to a salt which possesses the effectiveness of the parent compound and which is not biologically or otherwise undesirable (e. g, is neither toxic nor otherwise deleterious to the recipient thereof).

Suitable salts include acid addition salts which may, for example, be formed by mixing a solution of the compound of the present invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulfuric acid, acetic acid, trifluoroacetic acid, or benzoic acid. When the compounds of the invention carry an acidic moiety, suitable pharmaceutically acceptable salts thereof can include alkali metal salts (e. g., sodium or potassium salts), alkaline earth metal salts (e g., calcium or magnesium salts), and salts formed with suitable organic ligands such as quaternary ammonium salts. Also, in the case of an acid (-COOH) or alcohol group being present, pharmaceutical ty acceptable esters can be employed to modify the solubility or hydrolysis characteristics of the compound.

For the purpose of preventing or treating HIV infection or preventing, treating or delaying the onset of AIDS, the compounds of the present invention can be administered orally, parenterally (including subcutaneous injections, intravenous, intramuscular, intrastemal injection or infusion techniques), by inhalation spray, or rectally, in the form of a unit dosage of a pharmaceutical composition containing a therapeutically effective amount of the compound and conventional non-toxic pharmaceutically-acceptable carriers, adjuvants and vehicles.

The term"administration"and valiants thereof (e. g.,''administering"a compound) in reference to a compound of the invention mean providing the compound to the individual in need of treatment. When a compound of the invention is provided in combination with one or more other active agents (e. g., antiviral agents useful for treating HIV infection or AIDS), "administration"and its variants are each understood to include concurrent and sequential provision of the compound and other agents.

As used herein, the term"composition"is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combining the specified ingredients in the specified amounts.

By"pharmaceutically acceptable"is meant that the ingredients of the pharmaceutical composition must be compatible with each other and not deleterious to the recipient thereof.

The term"subject" (which may be alternatively referred to herein as"patient") as used herein refers to an animal, preferably a mammal, most preferably a human, who has been the object of treatment, observation or experiment.

The term"therapeutically effective amount"as used herein means that amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue, system, animal or human that is being sought by a researcher, veterinarian, medical doctor or other clinician, which includes alleviation of the symptoms of the disease being treated. When the active compound (i. e. , active ingredient) is administered as the salt, references to the amount of active ingredient are to the free acid or free base form of the compound.

The pharmaceutical compositions can be in the form of orally-administrable suspensions or tablets or capsules, nasal sprays, sterile injectible preparations, for example, as sterile injectible aqueous or oleagenous suspensions or suppositories. These compositions can be prepared by methods and contain excipients which are well known in the art. Suitable methods and ingredients are described in Remington's Pharmaceutical Sciences, ? 189 edition, edited by A.

R. Gennaro, Mack Publishing Co. , 1990, which is herein incorporated by reference in its entirety.

The compounds of this invention can be administered orally in a dosage range of 0. 001 to 1000 mg/kg of mammal (e. g., human) body weight per day in a single dose or in divided doses. One preferred dosage range is 0.01 to 500 mg/kg body weight per day orally in a single dose or in divided doses. Another preferred dosage range is 0.1 to 100 mg/kg body weight per day orally in single or divided doses. For oral administration, the compositions can be provided in the form of tablets or capsules containing 1.0 to 500 milligrams of the active ingredient, particularly 1,5, 10, 15,20, 25, 50, 75, 100, 150, 200, 250, 300,400, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated. The specific dose level and frequency of dosage for any particular patient may be varied and will depend upon a variety of factors including the activity of the specific compound employed, the metabolic stability and length of action of that compound, the age, body weight, general health, sex, diet, mode and time of administration, rate of excretion, drug combination, the severity of the particular condition, and the host undergoing therapy.

As noted above, the present invention is also directed to use of the HIV integrase inhibitor compounds of the present invention with one or more agents useful in the treatment of HTV infection or AIDS. For example, the compounds of this invention may be effectively administered, whether at periods of pre-exposure and/or post-exposure, in combination with effective amounts of one or more of the HIV/AIDS antivirals, imunomodulators, antiinfectives, or vaccines useful for treating HCV infection or AIDS. Suitable antiviral agents include those listed in the following Table : Drug Name Manufacturer Indication (Activitv) (Tradename and/or Location) abacavir Glaxo Welcome B infection, AIDS, ARC GW 1592 (ZIAGEN#) (nRTI) 1592U89 abacavir + lamivudine + GlaxoSmithKIine HIV infection, AIDS, ARC zidovudine (TRIZIVIR#) (nRTIs) acemannan Carrington Labs ARC (Irving, TX) ACH 126443 Achillion Phann. IRV infections, AIDS, ARC (NRTI) acyclovir Burroughs Welcome HIV infection, AIDS, ARC, in combination with AZT AD-439 Tanox Biosystems HIV infection, AIDS, ARC AD-519 Tanox Biosystems HIV infection, AIDS, ARC adefovir dipivoxil Gilead HIV infection, AIDS, ARC GS 840 (reverse transcriptase inhibitor) AL-721 Ethigen ARC, PGL, HIV positive, (Los Angeles, CA) AIDS alpha interferon GlaxoSmithKline Kaposi's sarcoma, HIV, in combination w/Retrovir AMD3100 AnorMed MV infection, AIDS, ARC (CXCR4 antagonist) amprenavir GlaxoSmithKline HIV infection, AIDS, 141 W94 (AGENERASE#) ARC (PI) GW 141 VX478 (Vertex) ansamycin Adria Laboratories ARC LM 427 (Dublin, OH) Erbamont (Stamford, CT) antibody which neutralizes Advanced Biotherapy AIDS, ARC pH labile alpha aberrant Concepts (Rockville, interferon MD) AR177 Aronex Pharm HIV infection, AIDS, ARC atazanavir (BMS 232632) 13ristol-Myers Squibb HIV infection, AIDS, ARC (REYATAZTM) (PI) beta-fluoro-ddA Nat'] Cancer Institute AIDS-associated diseases BMS-232623 Bristol-Myers Squibb/ HIV infection, AIDS, (CGP-73547) Novartis ARC (PI) BMS-234475 Bristol-Myers Squibb/FUV infection, AIDS, (CGP-61755) Novartis ARC (PI) capravirine Pfizer HIV infection, AIDS, (AG-1549, S-1153) ARC (nnRTI) CI-1012 Warner-Lambert HIV-1 infection cidofovir Gilead Science CMV retinitis, herpes, papillomavirus curdlan sulfate AJI Pharma USA HIV infection cytomegalovirns immune MedImmune CMV retinitis globin cytovene Syntex sight threatening CMV ganciclovir peripheral CMV retinitis delavirdine Pharmacia-Ugjohn HIV infection, AIDS, (RESCRIPTOR#) ARC (nnRTI) dextran Sulfate Ueno Pine Chem. Ind. AIDS, ARC, HIV Ltd. (Osaka, Japan) positive asymptomatic ddC Hoffman-La Roche HIV infection, AIDS, ARC (zalcitabine, (HIVID#) (nuclesodie reverse dideoxycytidine) transcriptase inhibitor) ddl Bristol-Myers Squibb HIV infection, AIDS, ARC ; (didanosine, (VIDEX#) combination with AZT/d4T dideoxyinosine) (nRTI) DPC 681 & DPC 684 DuPont EV infection, AIDS, ARC (PIs) DPC 961 & DPC 083 Bristol-Myers Squibb HIV infection AIDS, ARC (from DuPont Pharma) (nnRTIs) EL10 Elan Corp, PLC HIV infection (Gainesville, GA) efavirenz Bristol-Myers Squibb HIV infection, AIDS, (DMP 266) (SUSTIVA#) ARC (non-nucleoside RT Merck (STOCRIN#) inhibitor) famciclovir Novartis herpes zoster, herpes (FAMVIR) simplex emtricitabine Gilead (from Triangle HIV infection, AIDS, ARC FTC Pharmaceuticals) (nRTI) (COVIRACIL#) Emory University emvirine Gilead (from Triangle HFV infection, AIDS, ARC Pharmaceuticals) (nnRTI) (COACTINON#) enfuvirtide Trimeris & Roche HIV infection, AIDS, ARC T-20 (FUZEON#) (fusion inhibitor) HBY097 Hoechst Marion Roussel HIV infection, AIDS, ARC (nnRTD fosamprenavir Glaxo Smith Kline HIV infection, AIDS, ARC (prodrug of amprenavir) hypericin VIMRx Pharm. HIV infection, AIDS, ARC recombinant human Triton Biosciences AIDS, Kaposi's sarcoma, interferon beta (Almeda, CA) ARC interferon alfa-n3 Interferon Sciences ARC, AIDS indinavir Merck (CRIXTVAN@) HIV infection, AIDS, ARC, asymptomatic HTV positive, (PI) ISIS 2922 ISIS Pharmaceuticals CMV retinitis <BR> <BR> <BR> JE2147/AG1776 Agouron HIV infection, AIDS, ARC<BR> <BR> (PI) KNI-272 Nat'l Cancer Institute HIV-assoc. diseases lamivudine, 3TC GlaxoSmithKIine HIV infection, AIDS, (EPRVIRO) ARC (nRTI) lamivudine + zidovudine GlaxoSmithKline IHV infection, AIDS, (COMBVIR#) ARC (nRTI) lobucavir Bristol-Myers Squibb CMV infection lopinavir (ABT-378) Abbott HIV infection, AIDS, ARC (PI) lopinavir +ritonavir Abbott (KALETRA@) HLV infection, AIDS, ARC (ABT-378/r) (PI) mozenavix AVID (Camden, NJ) HIV infection, AIDS, ARC (DMP-450) (PI) nelfinavir Agouron HIV infection, AIDS, (VIRACEPT#) ARC (PI) nevirapine Boeheringer HIV infection, AIDS, Ingleheim ARC (nnRTI) (VIRAMUNE#) novapren Novaferon Labs, Inc. HIV inhibitor (Akron, OH) peptide T Peninsula Labs AIDS octapeptide (Belmont, CA) sequence PRO 140 Progenies HIV infection, AIDS, ARC (CCR5 co-receptor inhibitor) PRO 542 Progenies HIV infection, AIDS, ARC (attachment inhibitor) ttisodium Astra Pharm. Products, CMV retinitis, HIV infection, phosphonoformate Tnc other CMV infections PNU-140690 Pharmacia Upjohn HIV infection, AIDS, ARC (PI) probucol Vyrex HIV infection, AIDS RBC-CD4 Sheffield Med. Tech IHV infection, AIDS, (Houston TX) ARC ritonavir Abbott (NORVIR#) HIV infection, AIDS, (ABT-538) ARC (PI) saquinavir Hoffmann-LaRoche HIV infection, AIDS, (FORTOVASE#) ARC (PI) stavudine; d4T Bristol-Myers Squibb HIV infection, AIDS, ARC didehydrodeoxy- (ZERIT#) (nRTI) thymidine T-1249 Trimeris HIV infection, AIDS, ARC (fusion inhibitor) TAK-779 Takeda HIV infection, AIDS, ARC (injectable CCRS receptor antagonist) tenofovir Gilead (VIREAD#) HIV infection, AIDS, ARC (nucleotide reverse transcriptase inhibitor)

tipranavir (PNU-140690) Boehringer Ingelheim HIV infection, AIDS, ARC<BR> <BR> <BR> <BR> <BR> (PI) TMC-120 & TMC-125 Tibotec HIV infections, AIDS, ARC (nnRTIs) <BR> <BR> <BR> TMC-126 Tibotec HIV infection, AIDS, ARC<BR> <BR> <BR> <BR> <BR> (PI) valaciclovir GlaxoSmithKline genital HSV & CMV infections virazole Viratek/ICN (Costa asymptomatic HIV positive, ribavirin Mesa, CA) LAS, ARC zidovudine ; AZT GIaxoSmitKline HIV infection, AhDS, ARC, (R'ETROVIR) Kaposi's sarcoma in combination with other therapie (nRIl) PI = protease inhibitor nnRTI = non-nucleoside reverse transcriptase inhibitor nRTI-nucleoside reverse transcriptase inhibitor A compound of the present invention can also be administered in combination with an HIV integrase inhibitor such as a compound described in WO 99/62513, WO 99/62520, or WO 99/62897. A compound of the present invention can also be administered in combination with a CCR5 receptor antagonist, such as a compound described in WO 99/04794, WO 99/09984, WO 99/38514, WO 00/59497, WO 00159498, WO 00/59502, WO 00/59503, WO 00/765 1, WO 00/76512, WO 00/76513, WO 00176514, WO 00/76792, or WO 00/76793.

The compounds of this invention may be effectively administered, whether at peiiods of pre- exposure andlor post-exposure, in combination with effective amounts of one or more HIV/AIDS antivirals, immunomodulators, antiintectives, or vaccines useful for treating HIV infection or AIDS disclosed in the Table in WO 02/30930, which is herein incorporated by reference in its entirety.

It will be understood that the scope of combinations of the compounds of this invention with HIV/, AIDS antivirals, immunomodulators, anti-infectives or vaccines is not limited to those described or referenced above, but includes in principle any combination with any pharmaceutical composition useful for the treatment of AIDS. The HIV/AIDS antivirals and other agents will typically be employed in these combinations in their conventional dosage ranges and regimens as reported in the art, including the dosages described in the Physicians'

Desk Desk Reference, 54th edition, Medical Economics Company, 2000. The dosage ranges for a compound of the invention in these combinations are the same as those set forth above Abbreviations used in the instant specification, particularly the Schemes and Examples, include the following : AIDS=acquired immunodeficiency syndrome ARC = AIDS related complex Bn = benzyl DS-dimethylfom1amide DMSO = dimethyl sulfoxide ES-MS = eletron spray mass spectroscopy Et= ethyl HIV = human immunodeficiency vims HPLC = high performance liquid chromatography LC = liquid chromatography LiHMDS = lithium hexamethyldisilazide Me = methyl MS = mass spectroscopy NaHMDS = sodium hexamethyldisilazide NMR = nuclear magnetic resonance TFA = trifluoroacetic acid T = tetrahydrofuran The compounds of the present invention can be readily prepared according to the following reaction schemes and examples, or modifications thereof, using readily available starting materials, reagents and conventional synthesis procedures. In these reactions, it is also possible to make use of variants which are themselves known to those of ordinary skill in this art, but are not mentioned in greater detail. Furthermore, other methods for preparing compounds of the invention will be readily apparent to the person of ordinary skill in the art in light of the following reaction schemes and examples. Unless otherwise indicated, all variables are as defined above.

A general method for the preparation of compounds of the present invention embraced by Formula (I) is shown in Scheme 1, wherein piperazin-2-one 1-3 is treated with dialkylalkoxymethylenemalonate 1-4 and then with a deprotonating agent (e. g., Li or Na bis (trimethylsilyl) amide or Na hydride) at low temperature (e. g, from about 0 to about 25°C) in

an anhydrous non-protic solvent (eg., DB or THF) to give alkyl 8-hydroxy-1-oxo-1, 2,3, 4- tetrahydropyrrolopyrazine-7-carboxylate 1-6. The carboxylate 1-6 can be hydrolyzed to give the 7-carboxylic acid 1-7 Carboxylate 1 6 can also be treated with amine to give the 7-carboxamide 1-8 The piperazin-2-one 1-3 reactant can be obtained by alkylation of amine-protected piperazin-2-one 1-1 followed by deprotection to afford 1-3, as depicted in Scheme 1. Compound 1-1 can be prepared using methods described in Choi et al., J. Med. Chem. 1999, 3647; Najman- Bronzewska et al., Pharmazie 1997, 198 ; Fryer et al., J. Org. Chem. 1991, 3715, Dinsmore et al, Organic Prep. & Procedures International. 2002, 369, or routine variations thereof. An altemative method for preparing piperazin-2-one 1-3 is described in Bernotas et al., Tetrahedron tett. 1996,7339 ; Saati et al., J. Med. Chem. 1990, 2590 ; Sugihara et al, J. Med. Chem. 1998, 489, Dinsmore et al, Organic Prep. & Procedures Intemational. 2002,369, or routine variations thereof.

Some of the suitable dialkylalkoxymethylenemalonates 1-4 are commercially available (e. g., diethylethoxymethylenemalonate or dimethylmethoxy-methylenemalonate).

Others can be obtained by preparative methods known in the art ; e. g. , heterocyclylalkyloxymethylene malonates can be prepared by the method described in Boger et al. , J. Org. Chem 1988, 3408, or routine variations thereof.

The protection and deprotection of the amine in the piperazin-2-one can be accomplished using conventional amine protecting groups, such as those described in Protective Groups in Groups in Organic Chemistry, ed. J.F.W. McOmie, Plenum Press, 1973 and in T. W. Greene & P. G. M. Wuts, Protective Groups in Orsanic Synthesis. John Wiley & Sons, 1991.

SCHEME 1 FT PrO R3p 0 ri3 R4 Ii Ia R 4 0 r12 aminé R2 N. R* alkylation N-OR* deprotection HN I R= = C_6 alkyl or Bn Ri iN 0 1-1 0 1-2 RCO 4 R5 z3 z4 R5 C02RY M R4 6 R NH ! \) n l ! y Copy RY = alkyl, arylalkyl, or 0 1-3 heterocyclylalkyl 0 1-5 R3 R4 R5 R3 R4 R5 deprotonating R2 pRy R2 agent N N OH ( (-- 00 1-6 OH Oll ° 1"7 7-fi p OH o -, OH amidation with RURVNH R3 R4 R5 R2 N \ NR"RV Rt N . v0 oh OH

Scheme 2 exemplifies the same approach as set forth in Scheme I for the preparation of compounds embraced by Formula (II). In Scheme 2, 4-((aryl- or alkyl- oxy) carbonyl) piperazin-2-one 2-1 is alkylated with benzyl bromide 2-2 in the presence of a base (e. g., LiHMDS, NaHMDS, or NaH) to afford 1-benzyl-4-((aryl- or alkyl-oxy)carbonyl) piperazin- 2-one 2-3, which can be deprotected by standard methods (e-g., treatment with hydrogen) to afford 1-benzylpiperazin-2-one 2-4. Benzylpiperazinone 2-4 can then be reacted with

dialkylethoxymeAylenemalonate 2-S in a suitable solvent (e. X a hydrocarbon such as toluene) at elevated temperature (e. g., from about 60 to about 90°C) to afford the 2, 2-dialkyloxycarbonylethenyl-substituted product 2-6. Compound 2, 6 can then be treated with a deprotonating agent (e. g., LiHMDS) in an aprotic solvent (e. g., DMF) to provide the alkyl 2- benzyl-8-hydroxy-1-oxo-1,2, 3, 4-tetrahydropyrrolopyrazine-7-carboxylate 2-7. Carboxylate 2-7 can be converted to the corresponding acid 2-S by hydrolysis (e. g., with NaOH) and to the corresponding amide by treatment with an amine in the presence of a Lewis base (e. g., AlCl3).

SCHEME 2

x, 2 0 X2'0 , C. Br x. f'N'k OR* 2-2 N OR* HN, LIHMDS N LiHMDS,' N X = C1 e alkyl or Bn x11 2-3 0 2-3 O amine deprotection Et0 C02R 2-5 N rN CO R 2-5 C02R (R = C_4 alkyi 2,. 6 0 2-4 0 LiHMDS DMF x21 x2' hydrolysis_ N N , X/, I"' 2-7 o OFi X OH RUWNH AICI3 AIDS3 Xs N w 2-9 0 OH 2-9 0 OH

Scheme 3 illustrates a method forprepanng compounds of Formula (IV).

SCHEME 3 0 'it, amine C N OR* RZHal 94 amme R-Hay N-OR* deprotection HO LiHMDS RziN R* = Cy. g alky or Bn -1 O RZ = C alkyl Hal = CI, Br, or I ) J EO"CO, Et' !'DMF Et C02Et DMF R z,, N-- ? w"I N C02Et 3-2 O 3-3 0 u N OEt uRNH N N- AfCl3 ---t z'N Il 4 0 ow -5 0 or Ru = OH OH 3-5 0 OH RHorCa'kyt m Rv = subst'd alkyl pu u (e. g., arylalkyl) N I1 N Rz O 3-5

Scheme 4 is a variation of Scheme 3 and exemplifies the preparation of compounds of Formula (IV) with Rz = H. In Scheme 4, the piperazin-2-one 4-2 can be obtained by treatment of amine-protected piperazin-2-one 4-1 with base (e. g., LiHMDS, NaHMDS, or NaH) in DMF, followed by addition of methoxymethyl chloride or similar amide protecting group. Selective deprotection of the amine protecting group by hydrogenolysis provides piperazin-2-one 4-3. Treatment ofpiperazin-2-one 4-3 with diaLkylalkoxymethylenemalonate 1 4 and then with a deprotonating agent provides 8-hydroxy-1-oxo-1,2,3,4- tetrahydropyrrolopyrazxne-7-carboxylate 4-5. The carboxylate 4-5 can be hydrolyzed to give the 7-carboxylic acid 4-6, and deprotected to provide 4-7. Carboxylate 4-5 can also be treated with amine to give the 7-carboxamide 4-8, and deprotected to provide 4-9.

SCHEME 4 Ra R R iHMDS R R Ra J N OR* CH3oCH2Cl R * H2, PdIC R _---- FI N'IV R'= Bn I' 0 4-1 MeO 0 4-2 R DO2RY Fq3 R4 R Fl 3 R4 R5 NH l N R"= atkyl N C RY Rv = alkyl, arylalkyl, oY Me O 4-3 Me0 O 4-4 heterocyclylalkyl R'R"R' R5 R3 R4 R5 R3 R4 Rs agent R2 pRy (2 OH agent N \ hydrolysis N \ N w \ N w 0 MeO O OH MeO O OH 4-5 4-6 amidation with BBr3 R"NH 1 R3 R4 pz5 R3 R4 1 R OH R2 NF"Rv N \ H \ -IN" N so OH moo ° 4-8 OH 0 0 OH | BBr3 RA R4 Rs R2\4N ANRURV 0 4-9 HN. OH

In the processes for preparing compounds of the present invention set forth in the foregoing schemes, functional groups in various moieties and substituents may be sensitive or reactive under the reaction conditions employed and/or in the presence of the reagents employed.

Such sensitivity/reactivity can interfere with the progress of the desired reaction to reduce the yield of the desired product, or possibly even preclude its formation. Accordingly, it may be necessary or desirable to protect sensitive or reactive groups on any of the molecules concerned.

Protection can be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistry, ed. J. F_W, McOmie, Plenum Press, 1973 and in T. W.

Greene & P. G. M. Wuts, Protective Groups in Organic Synthesis. John Wiley & Sons, 1991. The

protective groups may be removed at a convenient subsequent stage using methods known in the art. The use of protective groups is illustrated in Scheme 4.

The following examples serve only to illustrate the invention and its practice. The examples are not to be construed as limitations on the scope or spirit of the invention.

EXAMPLE 1 Ethyl 2-benzyl-8-hydroxy-1-oxo-1, 2, 3,4-tetrahydropyrrolo[1,2-α]pyrazine-7-carboxylate

Step : Benzyl 4-benzyl-3-oxopiperazine-1-carboxylate

To a cold (0 °C) solution of benzyl 3-oxopiperazine-l-carboxylate (4.7 g, 20 mmol) in DMF (75 mL) under an atmosphere of nitrogen, a solution of lithium bis (tdmethylsilyl) amide in THF (24 mL, 24 mmol) was added and stirred at the temperature for 30 min. The resultant solution was treated with benzyl bromide (2, 9 24 rnmol), and stirred at room temperature overnight. The product mixture was concentrated under vacuum, and the residue partitioned between aqueous HCI and ethyl acetate. The organic extracted was washed with bnne, dried over anhydrous magnesium snifate, filtered, and concentrated under vacuum.

The residue was subjected to column chromatography on silica gel eluting with a 50-50 mixture of ethyl acetate and hexane. Collection and concentradon of appropriate fractions provided the benzylated product- 1H NMR (400 MHz, CDCl3) # 7. 4-7. 2 (m, 10H), 5.15 (s, 2H), 4.63 (s, 2H), 4.25 (s, 2H), 3.66 (br t, J= 5. 3 Hz, 2H), 3.27 (br s, 2H).

ES MS M+1 = 325 Step 2: 1-Benzylpiperazin-2-one

A mixture of benzyl 4-benzyl-3-oxopiperazine-1-carboxylate (4. 7 g, 14.5 mmol) and 10% Pd/C (0.47 g) in ethanol (150 mL) was stirred under an atmosphere of hydrogen (1 atm) at room temperature overnight. The product mixture was filtered through a pad of Celite, and concentrated under vacuum to provide 1-benzylpiperazin-2-one.

'li NMR (400 MHz, CDC),) 8 7. 4-7.2 (m, 5H), 4.61 (s, 2H), 3. 60 (s, 2H), 3.22 (t, J = 5.3 Hz, 2H), 3.03 (t, J = 5.3 Hz, 2H). <BR> <BR> <BR> <P>ES MS M+l = 191<BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> Step 3: Ethyl 2-benzyl-S-hydroxy-l-oxo-: L, 2,3, 4-tetrahydropyrrolo[1,2-α]-pyrazine-7- carboxylate A mixture of l-benzylpiperazin-2-one (0.29 g, 1. 54 mmol) and diethyl ethoxymethylenemalonate (0.35 g, 1. 63 mmol) in toluene was heated in a sealed tube at 80 °C for 4 hours. The resultant mixture was concentrated under vacuum, The residue was dissolved in anhydrous DMF (10 mL), cooled to 0 °C under an atmosphere of nitrogen, and treated with a solution of lithium bis (tzimethylsilyl) amide in THF (1 M, 2.0 mL, 2.0 mmol). The reaction mixture was stirred at room temperature overnight and concentrated under vacuum. The residue was partitioned between ethyl acetate and dilute aqueous HCI. The organic extract was washed with bline, dried over anhydrous magnesium sulfate, filtered, and concentrated under vacuum.

The residue was triturated with diethyl ether, and the solid precipitated was filtered to provide the title compound.

1H NMR (400 MHz, CDC13) 8 8.39 (br s, 1H), 7. 36-7.27 (m, 5H), 7. 03 (s, 1H), 4.67 (s, 2H), 4.31 (q, J = 7.1 Hz, 2H), 4.00 (t, J = 5.8 Hz, 2H), 3.51 (t, J = 5. 8 Hz, 2H), 1.35 (t, J = 7. 1 Hz, 3H). <BR> <BR> <BR> <P>ES M. S M+1= 315<BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> EXAMPLE 2 2-Benzyl-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolorl, 2-] pyrazine-7-carboxylic acid

A mixture of ethyl 2-benzyl-8-hydroxy-1-oxo-1,2,3,4-tetrahydropyrrolo-[1, 2- ] pyrazine-7-carboxylate (0.30 g ; see Example 1) and aqueous sodium hydroxide (1 M, 2 mL) in ethanol (20 mL) was heated in a sealed tube at 100 °C overnight. The product mixture was acidified with addition of TFA and concentrated under vacuum. The residue was subjected to SPLC puriacation on C-18 stationary phase eluted with water/acetonitrile/ TFA mobile phase.

Collection and lyophilization of appropriate fractions provided the title compound.

'H NMR (400 MHz, DMSO-d6) # 7. 37-7. 25 (m, 6H), 4. 62 (s, 2H), 4.09 (t, J = 5. 7 Hz, 2, 3.52 (t, J = 5.7 Hz, 2H).

ES MS M+1 = 287 EXAMPLE 3 Ethyl 2-(4-fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a.] pyrazine-7- carboxylate The title compound was prepared using a procedure similar to that described in Example 1, except that benzyl bromide (Step 1) was substituted with 4-fluorobenzyl bromide.

'H NMR (400 MHz, DMSO-d6) # 8. 54 (s, 1H), 7.40 (dd, J = 8. 3,5. 8 Hz, 2H), 7. 36 (s, 11-1), 7. 17 (t, J = 8.3 Hz, 2H), 4.59 (s, 2R, 4. 18 (q, J = 7.1 Hz, 2H), 4.01 (t, J = 5.3 Hz, 2H), 3.53 (t, J = 5. 3 Hz, 2li 1-24 (t, J = 7. 1 Hz, 3H).

ES MS M+1-333 EXEMPLE 4 2-(4-Fluorobenzyl)-8-hydroxy-1-oxo-1,2, 3, 4-tetrahydropyrrolo[1,2-a]pyrazine-7-carboxylic acid

The title compound was prepared using a procedure similar to that described in Example 2, except that ethyl 2-benzyl-8-hydroxy-1-oxo-1, 2, 3,4-tetrahydropyrrolo-[1, 2- a] pyrazine-7-carboxylate was substituted with ethyl 2-(4-fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4- tetahydropytrolo [l, 2-a]pyrazine-7-carboxylate.

'l-I NMR (400 MEz, DMSO-d6) # 8. 6 (br s, 1H). 7. 40 (dd, J = 8.3, 5. 8 Hz, 2H), 7.31 (s, 1H), 7.36 (s, 1H), 7.17 (t, J = 8.3 Hz, 2H), 4.59 (s, 211), 4. 09 (t, J = 5.5 Hz, 2H), 3.52 (t, 7= 5. 5 Hz, 2H).

ES MS M+1 = 305 EXAMPLE 5 2-(4-Fluorobenzyl)-8-hydroxy-N-methyl-1-oxo-1,2, 3, 4-tetrahydropyrrolo [1, 2-a]pyrazine-7- carboxamide Anhydrous methylamine gas was bubbled through a mixture of ethyl 2- (4- fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a]pyrazine-7-carboxylate (200 mg) and anhydrous aluminum chloride (200 mg) in anhydrous chloroform at 0 oC for 5 minutes. The resultant mixture was heated in a seal tube at 70 °C overnight and concentrated under vacuum.

The residue was dissolved in DMSO and acidified with TFA. The solution was subjected to HPLC purification on C-18 stationary phase eluted with water/acetonitrile/TFA mobile phase.

Collection and lyophilization of appropriate fractions provided the title compound.

1H NMR (400 MHz, DMSO-d6) 8 7.71 (br q, J= 4.6, 1R, 7.35 (dd, J = 8. 6,5. 7 Hz, 2H), 7. 24 (s, IR), 7. 17 (t, J = 8.6 Hz, 2H), 4.59 (s, 2H), 4.07 (t, J = 5.3 Hz, 2H), 3.50 (t, J = 5.3 Hz, 2H), 2.74 (s, 3H).

ESMSM+1318 EXAMPLE 6 2-(4-Fluorobenzyl)-8-hydroxy-N-ethyl-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1,2-a]pyrazine-7- carboxamide

The title compound was prepared using a procedure similar to that described in Example 5, except that methylamine was substituted with ethylamjne.

EI NMR (400 MHz. DMSO-d6) S 7 76 (br t, J = 5.3, 1H), 7.35 (dd, J = 8.7, 5. 6 Sz, 21-1), 7.27 (s, IM, 7.17 (t, J 8. 8 8 Hz, 2H), 4.59 (s, 2H), 4.07 (t, J = 5. 9 Hz, 2H), 3.50 (t, J= 5.9 Hz, 2H), 3.25 (m, 2E3J, 1.09 (t, J= 7.1 Hz, 3H).

ES MS M+l = 332 EXAMPLE 7 2-(4-Fluorobenzyl)-8-hydroxy-N-cyclopropyl-1-oxo-1,2, 3, 4-tetrahydropyrrolo [1, 2-a] pyrazine-7- carboxamide The title compound was prepared using a procedure similar to that described in Example 5, except that methylamine was substituted with cyclopropyl amine.

'H NMR (400 MHz, DMSO-d6) # 7.76 (br d, J = 3.2, 1H). 7.35 (dd, J = 8. 2,5. 6 Hz, 2H), 7.26 (s, 1H), 7. 17 (t, J = 8. 9 Hz, 2H), 4.59 (s, 2H), 4.06 (t, J = 5. 9 Hz, 2H), 3.50 (t, J = 5.9 Hz, 2H), 2. 75 (m, 1H), 0.69 (m, 2H), 0.49 (m, 2H).

ES MS M+1 = 344 EXAMPLES Ethyl 2- (3-chlorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a]pyrazine-7- carboxylate Step 1: 1-(3-chlorobenzyl)piperazin-2-one

To a cold (0 °C) solution of4- [ (-butoxy) carbonyl]-piperazm-2-one (4.0 g, 20 nunol) in DMF (175 mL) under an atmosphere of nitrogen, a solution of lithium bis (trimethylsilyl) amide in THF (22 mL, 22 mmol) was added and stirred at the temperature for 30 min. The resultant solution was treated with 3zchlorobenzyl bromide (2.6 mL, 20 mmol), and stirred at room temperature overnight. The product mixture was concentrated under vacuum, and the residue partitioned between 0.1 M aqueous HCl and ethyl acetate. The organic extracted was washed with brine, dried over anhydrous magnesium sulfate, filtered, and concentrated under vacuum. The residue was subjected to column chromatography on silica gel eluting with a 50-50 mixture of ethyl acetate and hexane-Collection and concentration of appropriate fractions provided the benzylated product-The product was dissolved in ethyl acetate (150 mL) and cooled to O °C A steady stream of anhydrous HCl gas was bubbled through for 10 minutes. The resultant mixture was capped and stirred at the same temperature for 1 hour. The product mixture was concentrated under vacuum. The residue was treated with chloroform saturated with ammonia, and the resultant suspension was filtered through a pad of Celite. The filtrate was concentrated under vacuum provided the title amine. Residual ammonia was removed by concentrating the residue three time with toluene under vacuum.

ES MS M+1 = 225 Step 2: Ethyl 2-(3-chlorobenzyl)-8-hydroxy-1-oxo-1,2,3,4-tetrahydropyrrolo [1,2-a]- pyrazine-7-carboxylate A mixture of 1-(3-¢hlorobenzyl) piperazin-2-onc (3. 97 g, 17.67 mmol) and diethyl ethoxymethylenemalonate (4. 01 g, 18. 55 mmol) in toluene (60 mL) was heated in a sealed tube at 88 °C overnight. The resultant mixture was concentrated under vacuum. The residue was

dissolved in anhydrous DMF (200 mL), cooled to 0 °C under an atmosphere of nitrogen, and treated with a solution of lithium bis (trimethylsily)amide in THF (1 M, 21.2 mL, 21. 2 mmol) The reaction mixture was stirred at room temperature overnight and concentrated under vacuum.

The residue was partitioned between ethyl acetate and dilute aqueous HCI. The organic extract was washed with brine, dried over anhydrous magnesium sulfate, filtered, and concentrated under vacuum. The residue was triturated with diethyl ether, and the solid precipitated was filtered to provide the title compound.

3H NMR (400 MHz, DMSO-d6) # 8. 60 (s, 1H), 7. 41-7. 28 (m, 5H), 4.62 (s, 2W7 4.19 (q, J = 7.2 Hz, 2H), 4. 11 (t, J = 5.7 Hz, 2H), 3.57 (t, J = 5.7 Hz, 2H), 1. 25 (t, J = 7.2 Hz, 3H).

ES MS M+1 = 349 EXAMPLE 9 2-(3-Chlorobenzyl)-8-hydroxy-1-oxo-1,2, 3, 4-tetrahydropyrolo[1,2-a]pyrazine-7-carboxylic acid The title compound was prepared using a procedure similar to that described in Example 2, except that ethyl 2-benzyl-8-hydroxy-1-oxo-1,2, 3, 4-tetrahydropyrrolo- [1, 2- a] pyrazine-7-carboxylate was substituted with ethyl 2-(3-chlorobenzyl)-8-hydroxy-1-oxo-1, 2, 3, 4- tetrahydropyrrolo[1, 2-a] pyrazine-7-carboxylate.

1H NMR (400 MHz, DMSO-d6) S 12 33 (br s, 1H), 8.69 (br s, 1H), 7A1-7. 27 (m, 5H), 4. 61 (s, 2H), 4-10 (t, J = 5. 3 Hz, 2H), 3.55 (t, J= 5.3 h7 2H) ES MS M+1 = 322 BXAMPLB 10 N- (4-Fluorobenzyl)-8-hydroxy-2-methyl-1-oxo-1, 2,'3, 4-tetrahydropyfrolo [1, 2-a] pyrazine-7- carboxamide Step l Methylpiperazin-2-one

The title compound was prepared using a procedure similar to that described in Example 1 (Step 1 & 2), except that benzyl bromide (Step 1) was substituted with methyl iodide.

ES MS M+1 = 115 Step 2 : Ethyl S-hydroxy-2-methyl-l-oxo-1, 2, 3, 4-tetrahydropyrroIo [1, 2-a] pyrazine-7- carboxylate The title compound was prepared using a procedure similar to that described in Example 1 (Step 3), except that 1-benzylpiperazin-2-one was substituted with 1-methylpiperazin- 2-one.

ES MS M+l-238 Step : N-(4-Fluorobenzyl)-8-hydroxy-2-methyl-1-oxo-1,2,3,4-tetrahyd ro-pyrrolo[1,2- a]pyrazine-7-carboxamide The title compound was prepared using a procedure similar to that described in Example 5, except that methylamine was substituted with 4-fluorobenyl-amine.

ES MS M+1 = 318 EXAMPLE 11 Ethyl 2-(3-fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyTTolo [1, 2-aXpyrazine-7- carboxylate

The title compound was prepared using a procedure similar to that described in Example 1, except that benzyl bromide (Step 1) was substituted with 3-fluorobenzyl bromide.

1H NMR (400 MHz, DMSO-d) 8 7. 36 (dd, J = 8, 8 Hz, 1H), 7.2-6.8 (m, 5 M, 4, 57 (s, 2H), 4. 05 (q, J = 7.1 Hz, 2H), 4.01 (t, J = 5.3 Hz, 2H), 3.53 (t, J = 5.3 Hz, 2H), 1. 18 (t, 7= 7. 1 Hz, 3H).

ES MS M+1 = 333 EXAMPLE 12 Ethyl 2-(3,4-fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a] pyrazine-7- carboxylate The title compound was prepared using a procedure similar to that described in Example 1, except that benzyl bromide (Step 1) was substituted with 3,4-difluorobenzyl bromide.

'IT NMR (400 MHz, DMSO-d6) # 7. 43-6. 82 (m, 5H), 4. 58 (s, 2H), 4.18 (q, J = 7. 0 Hz, 2H), 4. 02 (t, J = 5.3 Hz, 2H), 3.52 (t, J = 5.3 Hz, 2H), 1.23 (t, J = 7.0 Hz, 3H).

ES MS M+l = 351 EXAMPLE 13 Ethyl 2-(4-chlorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-a] pyrazine-7- carboxylate Step 1 : tert-Butyl 4- (4-chlorobenzyl)-3-oxopiperazinel-carboxylate

To a cold (0 °C) solution of tert-butyl 3-oxopiperazine-1-carboxylate (2.0 g, 9. 9 mmol) in DMF (100 mL) under an atmosphere of nitrogen, a solution of lithium bis (trimethylsilyl) amide in THF (10.9 mL, 10.9 mmol) was added and stirred at the temperature for 30 min. The resultant solution was treated with 4-chlorobenzyl bromide (2.1, 10.5 mmol), and stirred at room temperature overnight. The product mixture was concentrated under vacuum, and the residue partitioned between aqueous HC1 and ethyl acetate. The organic extracted was washed with brine, dried over anhydrous magnesium sulfate, filtered, and concentrated under vacuum. The residue was subjected to column chromatography on silica gel eluting with a mixture of ethyl acetate and dichloro-methane (0 to 50% gradient) Collection and concentration of appropriate fractions provided the benzylated product.

1H NMR (400 MHz, CDC13) 8 7.31 (br d, J = 8.5 Hz, 2H), 7.20 (br d, J = 8.5 Hz, 2H), 4.58 (s, 2H), 4.15 (s, 2H). 3.59 (br t, J = 5.3 Hz, 2H), 3.25 (br t, 2H).

ES MS M+l = 325 Step 2 : 1- (4-Chlorobenzyl)-piperazin-2-one A cold (0 °C) solution of tert-Butyl 4- (4-chlorobenzyl)-3-oxopiperazine-l- carboxylate (3. 2 g, 9.9 mmol) in ethyl acetate (100 mL) was saturated with HCI gas. The resultant mixture was stirred at 0 °C for 1 h. The product mixture was concentrated under vacuum. The residue was treated with dichloromethane saturated with ammonia gas. The resultant chalky mixture was filtered, and the filtrate concentrated inder vaccum. The residue was diluted with benzene and concentrated under vacuum to provide 1- (4-chlorobenzyl)- piperazin-2-one.

ES MS M+1 = 225 Step 3: Ethyl 2-(4-chlorobenzyl)-8-hydroxy-1-oxo-1,2, 3, 4-tetrahydropyrrolo[1, 2- a] pyrazme-7-carboxylate

A mixture of 1- (4-chlorobenzyl)-piperazin-2-one (1.94 g, 8. 63 mmol) and diethyl ethoxymethylenemalonate (1. 87 g, 8.63 mmol) in toluene (40 mL) was heated in a sealed tube at 80 °C ovemight The resultant mixture was concentrated under vacuum-The residue was dissolved in anhydrous DMF IF (50 mL), cooled to 0 °C under an atmosphere of nitrogen, and treated with a solution of lithium bis (trimethylsilyl) amide in THF (1 M, 12 mL, 12 mmol). The reaction mixture was stirred at room temperature overnight and concentrated under vacuum. The residue was partitioned between ethyl acetate and dilute aqueous HCI. The organic extract was washed with bnne, dried over anhydrous magnesium sulfate, filtered, and concentrated under vacuum. The residue was subjected to HPLC purification on C-18 stationary phase eluted with water/acetonitrile/ TFA mobile phase. Collection and lyophilization of appropriate fractions provided the title compound.

'H NMR (400 MHz, DMSO-d6) # 7.37 (d, J = 8.3, 2H), 7.30 (d, J = 8.3, 2H), 7.09 (s, 1H), 4.55 (s, 2H), 4.05 (q, J = 7.1 Hz, 2H) 4.01 (br t, 2H), 3. 53 (br t, 2H), 1.19 (t, J = 7. 1 Hz, 3H).

ES MS M+1 = 349 EXAMPLE 14 Ethyl 2-(3-chloro-4-fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo[1,2-a]pyrazine-7- carboxylate

The title compound was prepared using a procedure similar to that described in Example 13, except that 4-chlorobenzyl bromide (Step 1) was substituted with 3-chloro-4- fluorobenzyl bromide.

'H NMR (400, DMSO-d6) 8 8.58 (s, IM, 7. 4-7. 3 (m, 4 H), 4.59 (s, 2H). 4. 18 (q, J = 7.1 Hz, 2H), 4.11 (t, J = 5.3 Hz, 2H), 3.56 (t, J= 5w3 Hz, 2H), 1.24 (t, J = 7. 1 Hz, 3rI).

ESMSM+1=367 EXAMPLE 15 Ethyl 2-(3,4-dichlorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [l, 2-a] pyrazine-7- carboxylate

The title compound was prepared using a procedure similar to that described in Example 13, except that 4-chlorobenzyl bromide (Step 1) was substituted with 3,4- dichlorobexazyl bromide.

1H NMR (400 At DMSO-d6) 6 8. 59 (s, 1H), 7.61 (d, J = 8.4 Hz, 1H), 7. 58 (d, J = 1.8 Hz, 1H), 7.37 (s, IM, 7. 31 (dd, J= 8. 4, 1.8 Hz, 1H), 4.60 (s, 2H), 4.18 (q, J = 7.0 Hz, 2H), 4.11 (t, J= 5. 8 Hz, 2H), 3. 57 (t, J = 5.8 Hz, 2H), 1.24 (t, J = 7. 0 Hz, 3H).

ES MS M+1 = 383 EXAMPLE 16 2-(4-Chlorobenzyl)-8-hydroxy-N-methyl-1-oxo-1,2,3,4-tetrahyd ropyrolo[1,2-a]pyrazine-7- carboxamide

The title compound was prepared using a procedure similar to that described in Example 5, except that ethyl 2-(4-fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2-

a]pyrazine-7-carboxylate was subsituted with ethyl 2-(4-chlorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4- tetrahydropyrrolo[1,2-a]pyrazine-7-carboxylate (Example 13).

1H NMR (400 MHz, CDC13) 8 7.33 (d, J= 8. 2, 1H), 7.27-7. 21 (m), 7.18 (s, 1H), 4.65 (s, 2H), 4.03 (t, J = 6.3 Hz, 2H), 3.53 (t, J = 6.3 Hz, 2H), 2.99 (d, J = 4. 8 Hz, 3H).

ES MS M+l = 334 EXAMPLE 17 2-(3,4-Difluorobenzyl)-8-hydroxy-N-methyl-1-oxo-1, 2,3, 4-tetrahydropynolo [1, 2-a] pyrazine-7- carboxamide

The title compound was prepared using a procedure similar to that described in Example 5, except that ethyl 2-(4-fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo[1, 2- a] pyrazine-7-carboxylate was subsituted with ethyl 2-(3,4-difluorobenzyl)-8-hydroxy-1-oxo- 1, 2,3, 4-tetrahydropyrrolo[1,2-a]pyrazine-7-carboxylate (Example 12). xi NMR (400 MHz, CDCl3) s 7. 34-7. 05 (m), 6. 74 (br s, 1H), 4.64 (s, 2H), 4.05 (t, J = 5. 7 Hz, 2H), 3. 54 (t, J = 5.7 Hz, 2H), 2.99 (d, J = 4.6 Hz, zu ES MS M+1 = 336 EXAMPLE 18 243, 4-Dichlorobenzyl)-8-hydroxy-N-methyl-l-oxo-1, 2, 3,4-tetrahydropyrrolo [1, 2-a] pyrazine-7- carboxamide

The title compound was prepared using a procedure similar to that described in Example 5, except that ethyl 2-(4-fluorobenzyl)-8-hydroxy-1-oxo-1, 2,3, 4-tetrahydropyrrolo [1, 2- a] pyrazine-7-carboxylate was subsituted with ethyl 2-(3,4-dichlorobenayl)-8-hydroxy-1-oxo- 1, 2,3, 4-tetrahydropyrrolo[1,2-a]pyrazine-7-carboxylate (Example 15).

IH NMR (400 MHz, CDCl3) # 7. 43 (d, J = 8.4 Hz, 1H), 7.40 (d, J = 1.8 Hz, 1H), 7. 19 (s, 1H), 7.16 (dd, J = 8. 4, 1. 8 Hz, 1H), 4 64 (s, 2R, 4.05 (t, J = 5. 7 Hz, 2H), 3.54 (t, J = 5.7 Hz, 2H), 2.99 (d, J=4. 6Hz, 3H).

ESMSM+1=368 EXAMPLE 19 Oral Compositions As a specific embodiment of an oral composition of a compound of this invention, 50 mg of compound of Example 1 is formulated with sufficient finely divided lactose to provide a total amount of 580 to 590 mg to fill a size 0 hard gelatin capsule. Encapsulated oral compositions containing any one of the compounds of Examples 2-18 can be similarly prepared.

EXAMPLE 20 HIV Integrase Assay : Strand Transfer Catalyzed zed by Recombnant Integrase Assays for the strand transfer activity of integrase were conducted in accordance with WO 02/30930 for recombinant integrase. Representative compounds of the present invention exhibit inhibition of strand transfer activity in this assay. For example, the compounds prepared in Examples 1-18 were tested in the integrase assay and all were found to have IC50's less than 1. 5 micromolar In particular, the compounds prepared in Examples 1-10 were all found to have IC50's less than 0.7 micromolar in the integrase assay.

Further description on conducting the assay using preassembled complexes is found in Wolfe, A.L. et al., J. Virol. 1996, 70: 1424-1432, Hazuda et al., J. Virol. 1997, 71 : 7005-7011; Hazuda et al., Drug Design and Discovery 1997, 15: 17-24; and Hazuda et al., Science 2000,287 : 646-650.

EXAMPLE 21 Assay for inhibition of HEV replication Assays for the inhibition of acute HIV infection of T-lymphoid cells were conducted in accordance with Vacca, J. P. et al, Proc. Natl. Acad. Sci. USA 1994, 91 : 4096.

Representative compounds of the present invention exhibit inhibition of IUV replication in this assay. For example, the compounds prepared in Examples 1,3, 5-8,10, 16 and 17 were found to have IC95's at or less than 20 micromolar in the present assay.

While the foregoing specification teaches the principles of the present invention, with examples provided for the purpose of illustration, the practice of the invention encompasses all of the usual variations, adaptations and/or modifications that come within the scope of the following claims.