Title:
AFFINITY-BASED ENRICHMENT OF PHOSPHORYLATED PEPTIDES AND/OR PROTEINS
Document Type and Number:
WIPO Patent Application WO2004099233
Kind Code:
A3
Abstract:
The invention relates to a substance comprising a solid carrier that is connected to a spacer by means of a linker, said spacer comprising at least two defined groups. Said substance is suitable for using as an affinity material for enriching and/or isolating phosphorylated peptides and/or proteins. The inventive substance especially enables tyrosine-phosphorylated peptides and/or proteins to be enriched and/or isolated.
Inventors:
SOSKIC VUKIC (GB)
SCHRATTENHOLZ ANDRE (DE)
SCHRATTENHOLZ ANDRE (DE)
Application Number:
PCT/EP2004/004405
Publication Date:
January 27, 2005
Filing Date:
April 27, 2004
Export Citation:
Assignee:
PROTEOSYS AG (DE)
SOSKIC VUKIC (GB)
SCHRATTENHOLZ ANDRE (DE)
SOSKIC VUKIC (GB)
SCHRATTENHOLZ ANDRE (DE)
International Classes:
B01J20/289; C07K1/22; B01D15/38; (IPC1-7): B01J20/32
Foreign References:
| US5185313A | 1993-02-09 |
Other References:
PORATH J ET AL: "CASCADE-MODE MULTIAFFINITY CHROMATOGRAPHY FRACTIONATION OF HUMAN SERUM PROTEINS", JOURNAL OF CHROMATOGRAPHY, vol. 550, no. 1-2, 1991, pages 751 - 764, XP009039714
WINZERLING JOY J ET AL: "Purification of recombinant insect transferrin from large volumes of cell culture medium using high capacity Ni-2+-dipicolylamine gel", PROTEIN EXPRESSION AND PURIFICATION, vol. 7, no. 2, 1996, pages 137 - 142, XP002305179, ISSN: 1046-5928
PORATH J: "IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, US, vol. 3, no. 4, August 1992 (1992-08-01), pages 263 - 281, XP001098444, ISSN: 1046-5928
OJIDA AKIO ET AL: "First artificial receptors and chemosensors toward phosphorylated peptide in aqueous solution", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 124, no. 22, 5 June 2002 (2002-06-05), pages 6256 - 6258, XP002305180, ISSN: 0002-7863
MITO-OKA Y ET AL: "Zn(II) dipicolylamine-based artificial receptor as a new entry for surface recognition of alpha-helical peptides in aqueous solution", TETRAHEDRON LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 42, no. 40, 1 October 2001 (2001-10-01), pages 7059 - 7062, XP004317891, ISSN: 0040-4039
WINZERLING JOY J ET AL: "Purification of recombinant insect transferrin from large volumes of cell culture medium using high capacity Ni-2+-dipicolylamine gel", PROTEIN EXPRESSION AND PURIFICATION, vol. 7, no. 2, 1996, pages 137 - 142, XP002305179, ISSN: 1046-5928
PORATH J: "IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, US, vol. 3, no. 4, August 1992 (1992-08-01), pages 263 - 281, XP001098444, ISSN: 1046-5928
OJIDA AKIO ET AL: "First artificial receptors and chemosensors toward phosphorylated peptide in aqueous solution", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 124, no. 22, 5 June 2002 (2002-06-05), pages 6256 - 6258, XP002305180, ISSN: 0002-7863
MITO-OKA Y ET AL: "Zn(II) dipicolylamine-based artificial receptor as a new entry for surface recognition of alpha-helical peptides in aqueous solution", TETRAHEDRON LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 42, no. 40, 1 October 2001 (2001-10-01), pages 7059 - 7062, XP004317891, ISSN: 0040-4039
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