α-AMYLASE VARIANT

Title:

α-AMYLASE VARIANT

Document Type and Number:

WIPO Patent Application WO/2023/176970

Kind Code:

A1

Abstract:

Provided is an α-amylase which shows an excellent starch decomposition activity at lower temperatures and also shows excellent stability at a low pH value and/or in the presence of a chelating agent.　Provided is an α-amylase variant which is a variant of a parent α-amylase, the α-amylase variant including the amino acid residue substitution at a position corresponding to H238-position or E185-positin in the amino acid sequence represented by SEQ ID NO: 2 and the amino acid residue substitution mentioned in (A) or (B) below, in which the parent α-amylase or the α-amylase variant has at least 80% sequence identity to the amino acid sequence represented by SEQ ID NO: 2 (excluding an α-amylase variant having amino acid residue substitutions at three positions corresponding to H238-position, S239-position and G178-position in the aforementioned amino acid sequence, an α-amylase variant having amino acid residue substitutions at three positions corresponding to H238-position, R209-position and G178-position in the aforementioned amino acid sequence, and an α-amylase variant having amino acid residue substitutions at three positions corresponding to E185-position, N190-position and G178-position in the aforementioned amino acid sequence).　(A) The amino acid residue substitution at at least one position selected from positions respectively corresponding to T116-position, A181-position, A199-position, A275-position, V277-position, A286-position and L323-position in the amino acid sequence represented by SEQ ID NO: 2; and (B) the amino acid residue substitution at a position corresponding to G178-position in the amino acid sequence represented by SEQ ID NO: 2 and the amino acid residue substitution at at least one position selected from N126-position, T129-position, N190-position, R209-position and S239-position in the aforementioned amino acid sequence.

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Inventors:

SHAKU MAO (JP)
HIOKI TAKAHIRO (JP)
TERAI MIKA (JP)
KAWAHARA AKIHITO (JP)

Application Number:

PCT/JP2023/010681

Publication Date:

September 21, 2023

Filing Date:

March 17, 2023

Export Citation:

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Assignee:

KAO CORP (JP)

International Classes:

C12N9/26; C11D3/386; C11D17/06; C11D17/08; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/28; C12N15/10; C12N15/31; C12N15/56; C12N15/63

Domestic Patent References:

WO2022014428A1

2022-01-20

Foreign References:

US20210301223A1	2021-09-30
JP2021505167A	2021-02-18

Other References:

KACHAN ALEXANDR V.; EVTUSHENKOV ANATOLIY N.: "Thermostable mutant variants of Bacillus sp. 406 a-amylase generated by site-directed mutagen", CENTRAL EUROPEAN JOURNAL OF BIOLOGY, WARSAW : VERSITA, PL, vol. 8, no. 4, 9 February 2013 (2013-02-09), PL , pages 346 - 356, XP035347957, ISSN: 1895-104X, DOI: 10.2478/s11535-013-0142-0

Attorney, Agent or Firm:

ARUGA PATENT OFFICE (JP)

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