Title:
APPLICATION OF NON-INVASIVE ULTRASONIC CELL IN PREPARING EXOSOME, EXOSOME, AND PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/125447
Kind Code:
A1
Abstract:
Provided is a preparation method for an exosome. A cell is stimulated by means of non-invasive ultrasound to regulate the microRNA expression abundance in the cell exosome. The method has good universality, is simple in operation, and has a large yield of exosome. A target exosome can be obtained from the method in short time. The obtained exosome can be used for improving the expression of a brain-derived neurotrophic factor, promoting, clearing a harmful protein from the brain, or reducing the oxidative damage of a harmful substance to the brain tissue.
Inventors:
ZHENG HAIRONG (CN)
YAN FEI (CN)
DENG ZHITING (CN)
XIAO YANG (CN)
YAN FEI (CN)
DENG ZHITING (CN)
XIAO YANG (CN)
Application Number:
PCT/CN2019/123568
Publication Date:
June 25, 2020
Filing Date:
December 06, 2019
Export Citation:
Assignee:
SHENZHEN INST ADV TECH (CN)
International Classes:
C12N5/079; A61K35/30; A61P9/10; A61P25/00; C12N13/00
Domestic Patent References:
| WO2012054975A1 | 2012-05-03 |
Foreign References:
| CN109554341A | 2019-04-02 | |||
| CN108624557A | 2018-10-09 | |||
| CN107106615A | 2017-08-29 | |||
| CN108865983A | 2018-11-23 |
Other References:
郭莹 等 (GUO, YING ET AL.): "人脐带间充质干细胞来源外泌体提取方法的比较 (Comparison of Exosome Extracting Methods from Human Umbilical Cord Mesenchymal Stem Cells)", 中国组织工程研究 (CHINESE JOURNAL OF TISSUE ENGINEERING RESEARCH), vol. 22, no. 9, 30 September 2018 (2018-09-30), ISSN: 2095-4344, DOI: 20200226112839A
MURACA MAURIZIO ET AL.: "Exosome Treatment of Bronchopulmonary Dysplasia: How Pure Should Your Exosome Preparation Be", AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 21 November 2017 (2017-11-21), XP055715984, ISSN: 1073-449X, DOI: 20200226112452A
MURACA MAURIZIO ET AL.: "Exosome Treatment of Bronchopulmonary Dysplasia: How Pure Should Your Exosome Preparation Be", AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 21 November 2017 (2017-11-21), XP055715984, ISSN: 1073-449X, DOI: 20200226112452A
Attorney, Agent or Firm:
SHENZHEN KEJIN INTELLECTUAL PROPERTY OFFICE (CN)
Download PDF:
Previous Patent: DEFROSTING APPARATUS
Next Patent: DIFFERENTIALLY EXPRESSED GENE SCREENING METHOD AND DEVICE
Next Patent: DIFFERENTIALLY EXPRESSED GENE SCREENING METHOD AND DEVICE