Title:
BACILLUS LICHENIFORMIS CELL CAPABLE OF BEING STABLY AND REPEATEDLY USED FOR CONVERSION AND SYNTHESIS OF D-ALLULOSE
Document Type and Number:
WIPO Patent Application WO/2024/067086
Kind Code:
A1
Abstract:
Provided is a Bacillus licheniformis cell capable of being stably and repeatedly used for conversion and synthesis of D-allulose, which belongs to the field of biotechnology. D-allulose is a functional sugar with a high health value and has wide application prospects in the fields of food, medicine, health care, and the like. The Bacillus licheniformis cell efficiently expresses a recombinant D-allulose-3-epimerase and can convert high-concentration fructose that serves as a substrate to generate D-allulose. After the whole-cell catalyst is repeatedly used 10 times, the conversion rate is not lower than 30%. Compared with a pure enzyme or a one-time whole-cell conversion mode, this technology for synthesizing D-allulose has remarkable advantages of low catalyst use cost, simple separation and purification, and the like.
Inventors:
LI YOURAN (CN)
WU ZHIYONG (CN)
SHI GUIYANG (CN)
WU ZHIYONG (CN)
SHI GUIYANG (CN)
Application Number:
PCT/CN2023/118453
Publication Date:
April 04, 2024
Filing Date:
September 13, 2023
Export Citation:
Assignee:
WUXI INST FOR SPECIALIZED NUTRITION AND HEALTH CO LTD (CN)
International Classes:
C12N15/61; C12N1/21; C12N15/75; C12P19/02; C12R1/10
Foreign References:
| CN116064619A | 2023-05-05 | |||
| CN116676166A | 2023-09-01 | |||
| CN112695006A | 2021-04-23 | |||
| CN108102995A | 2018-06-01 | |||
| CN111032879A | 2020-04-17 | |||
| US20180112244A1 | 2018-04-26 |
Other References:
JIA MIN: "Expression of D-Psicose 3-Epimerase in Bacillus subtilis", JOURNAL OF FOOD SCIENCE AND BIOTECHNOLOGY, vol. 33, no. 11, 15 November 2014 (2014-11-15), pages 1129 - 1135, XP093154244
RAO,Y. ET AL.: "Construction and application of a dual promoter system for efficient protein production and metabolic pathway enhancement in Bacillus licheniformis", JOURNAL OF BIOTECHNOLOGY, 28 February 2020 (2020-02-28), pages 1 - 10, XP086092025, DOI: 10.1016/j.jbiotec.2020.02.015
RAO,Y. ET AL.: "Construction and application of a dual promoter system for efficient protein production and metabolic pathway enhancement in Bacillus licheniformis", JOURNAL OF BIOTECHNOLOGY, 28 February 2020 (2020-02-28), pages 1 - 10, XP086092025, DOI: 10.1016/j.jbiotec.2020.02.015
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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