Title:
BICISTRONIC EXPRESSION VECTOR FOR EXPRESSING ANTIBODY AND METHOD FOR PRODUCING ANTIBODY USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2013/180473
Kind Code:
A1
Abstract:
The present invention relates to a bicistronic expression vector for expressing an antibody, comprising a first expression cassette comprising 'promoter-intron-antibody light chain gene -polyA tail' and a second expression cassette comprising 'promoter-intron-antibody heavy chain gene-internal ribosome entry site (IRES)-amplification gene-polyA tail', an animal cell transfected with the expression vector, and a method for producing the antibody comprising culturing the animal cell. Based on the bicistronic expression vector for producing antibody comprising an intron of the present invention, an expression vector capable of expressing a desired antibody at high efficiency can be prepared. Also, through culturing the animal cells transfected with the expression vector, the desired antibody can be expressed stably and at high efficiency.
Inventors:
KIM SUN KYU (KR)
LEE DONG HEON (KR)
JANG HYUN SOOK (KR)
JUNG SUN KI (KR)
YOON JI YONG (KR)
KIM WON KYUM (KR)
MOON KYUNG DUK (KR)
AHN YONG HO (KR)
PARK SANG KYUNG (KR)
LEE DONG HEON (KR)
JANG HYUN SOOK (KR)
JUNG SUN KI (KR)
YOON JI YONG (KR)
KIM WON KYUM (KR)
MOON KYUNG DUK (KR)
AHN YONG HO (KR)
PARK SANG KYUNG (KR)
Application Number:
PCT/KR2013/004725
Publication Date:
December 05, 2013
Filing Date:
May 29, 2013
Export Citation:
Assignee:
HANWHA CHEMICAL CORP (KR)
International Classes:
C12N15/85; C07K16/00; C12N5/10; C12P21/08
Foreign References:
| KR20030062118A | 2003-07-23 |
Other References:
FALLOT, S. ET AL.: "Alternative-splicing-based bicistronic vectors for ratio-controlled protein expression and application to recombinant antibody production, e134", NUCLEIC ACIDS RESEARCH, vol. 37, no. 20, 3 September 2009 (2009-09-03)
LUCAS, B. K. ET AL.: "High-level production of recombinant proteins in CHO cells using a dicistronic DHFR intron expression vector", NUCLEIC ACIDS RESEARCH, vol. 24, no. 9, 1996, pages 1774 - 1779
MARTINEAU, Y. ET AL.: "Internal ribosome entry site structural motifs conserved among mammalian fibroblast growth factor 1 alternatively spliced mRNAs", MOL. CELL. BIOL., vol. 24, no. 17, September 2004 (2004-09-01), pages 7622 - 7635
LUCAS, B. K. ET AL.: "High-level production of recombinant proteins in CHO cells using a dicistronic DHFR intron expression vector", NUCLEIC ACIDS RESEARCH, vol. 24, no. 9, 1996, pages 1774 - 1779
MARTINEAU, Y. ET AL.: "Internal ribosome entry site structural motifs conserved among mammalian fibroblast growth factor 1 alternatively spliced mRNAs", MOL. CELL. BIOL., vol. 24, no. 17, September 2004 (2004-09-01), pages 7622 - 7635
Attorney, Agent or Firm:
SON, Min (36 Teheran-ro 87 gil,Gangnam-gu, Seoul 135-973, KR)
Download PDF: