Title:
BIFUNCTIONAL C-GLYCOSIDE GLYCOSYLTRANSFERASES AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/164673
Kind Code:
A1
Abstract:
The present invention provides a group of novel uridine diphosphate (UDP)-glycosyltransferases, which are bifunctional C-glycoside arabinosyltransferases and C-glycoside glucosyltransferases. The glycosyltransferases can specifically and efficiently catalyze C-glycoside arabinosylation and glucosylation of dihydrochalcone compounds or 2-hydroxyflavanone compounds, to generate C-glycoside dihydrochalcone or C-glycoside-2-hydroxyflavanone compounds; the C-glycoside-2-hydroxyflavanone compounds are further subjected to a dehydration reaction to form flavonoid C-glycoside compounds. The present invention also provides an application of the novel UDP-glycosyltransferases to artificially constructed recombinant expression systems to generate C-glycoside dihydrochalcone and flavonoid C-glycoside compounds by means of fermentation engineering.
Inventors:
WANG YONG (CN)
SUN YUWEI (CN)
CHEN ZHUO (CN)
SUN YUWEI (CN)
CHEN ZHUO (CN)
Application Number:
PCT/CN2021/076398
Publication Date:
August 26, 2021
Filing Date:
February 09, 2021
Export Citation:
Assignee:
CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES (CN)
International Classes:
C12P19/18; C12N5/10; C12N15/54
Foreign References:
| CN109097377A | 2018-12-28 | |||
| CN105247045A | 2016-01-13 |
Other References:
DATABASE PROTEIN ANONYMOUS: "C-glycosyltransferase [Phyllostachys edulis]", XP055832003, retrieved from GENBANK
WANG, YUANJIE, GUO XUEFENG ZHAO LEI GUO CHENG WANG YUWEI: "Characterization of the C-glycosylation Pathway and C-glucosyltransferase of C-glycosyl Flavonoids from Phyllostachys edulis Leaves", SCIENTIA SILVAE SINICAE, vol. 54, no. 12, 1 December 2018 (2018-12-01), pages 1 - 10, XP055838570, DOI: 10．11707 /j．1001-7488．20181207
Science Press; "GenBank", Database accession no. XP_008660140.1
WANG, YUANJIE, GUO XUEFENG ZHAO LEI GUO CHENG WANG YUWEI: "Characterization of the C-glycosylation Pathway and C-glucosyltransferase of C-glycosyl Flavonoids from Phyllostachys edulis Leaves", SCIENTIA SILVAE SINICAE, vol. 54, no. 12, 1 December 2018 (2018-12-01), pages 1 - 10, XP055838570, DOI: 10．11707 /j．1001-7488．20181207
Science Press; "GenBank", Database accession no. XP_008660140.1
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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