Title:
BILAYER CULTURE SUBSTRATE
Document Type and Number:
WIPO Patent Application WO/2022/255251
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a cell culture substrate, which is suitable for the production of an in vitro biological tissue model of a biological tissue having a gas-liquid interface and which enables temporal and real-time observation under an optical microscope, and a method for producing the same; and a biological tissue model produced by using the cell culture substrate and a method for producing the same. While wetting with an aqueous solvent, a light-permeable cellulose derivative membrane is made porous to thereby impart water absorption properties suitable for a culture substrate thereto. This porous cellulose derivative membrane is combined with polymer microfibers to give a transparent bilayer substrate as a cell culture substrate. Further, cells are cultured at a gas-liquid interface using the transparent bilayer substrate to give an in vitro biological tissue model having characteristics similar to a biological tissue.
Inventors:
TAKEDA NAOYA (JP)
TSUCHIDO YUJI (JP)
ONUKI MAI (JP)
TSUCHIDO YUJI (JP)
ONUKI MAI (JP)
Application Number:
PCT/JP2022/021748
Publication Date:
December 08, 2022
Filing Date:
May 27, 2022
Export Citation:
Assignee:
UNIV WASEDA (JP)
International Classes:
C12M3/00; C12M1/00; C12N1/00; C12N5/071
Foreign References:
| JP2017197874A | 2017-11-02 | |||
| JP2010502855A | 2010-01-28 | |||
| JP2019162818A | 2019-09-26 | |||
| JPH0838165A | 1996-02-13 | |||
| JPH03175973A | 1991-07-31 |
Attorney, Agent or Firm:
USHIKI & ASSOCIATES (JP)
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