Title:
BIOLOGICAL AGE REVERSING PREPARATION AND METHOD FOR PREPARING SAME
Document Type and Number:
WIPO Patent Application WO/2023/216341
Kind Code:
A1
Abstract:
A method for preparing a biological age reversing preparation, comprising the steps of: placing cytotoxic T lymphocytes, a cell mitogen, a cytokine, and an immune adjuvant in a liquid cell culture medium in a culture container for co-culture to obtain an immune cell culture, and isolating a population of cells with immune activity from the immune cell culture to obtain the biological age reversing preparation. By using the biological age reversing preparation, the biological age can be reversed significantly. The biological age reversing preparation comprising 10 10 cytotoxic T lymphocytes is used three times within a week, resulting in 1.3 years of biological age reversal after the preparation is continuously used for 3 months, and 4.5 years of biological age reversal after the preparation is continuously used for 9 months.
Inventors:
HONG JIXIAN (CN)
LIU HUA (CN)
LIU HUA (CN)
Application Number:
PCT/CN2022/096096
Publication Date:
November 16, 2023
Filing Date:
May 30, 2022
Export Citation:
Assignee:
HONG JIXIAN (CN)
International Classes:
C12N5/0783; A61K35/17; A61P37/02; C12Q1/6874; C12Q1/6888
Domestic Patent References:
| WO2022032665A1 | 2022-02-17 |
Foreign References:
| CN114908051A | 2022-08-16 | |||
| CN111876382A | 2020-11-03 | |||
| CN102847144A | 2013-01-02 | |||
| CN102847143A | 2013-01-02 | |||
| US4950647A | 1990-08-21 |
Other References:
MIN JIANG: " A Study on the Correlation Between the Distribution Pattern of CD4+CD25+ Regulatory T Cells and Age and Gender in Healthy Population", FUJIAN MEDICAL JOURNAL, vol. 33, no. 6, 28 December 2011 (2011-12-28), pages 96 - 97, XP093109366
XIN LI: "Progress in the Study of the Correlation Between DNA Methylation and Individual Age", THE JOURNAL OF LAW & MEDICINE, vol. 13, no. 4, 20 November 2006 (2006-11-20), pages 284 - 288, XP093109369
HEXIANG PENG, GAO WENJING, CAO WEIHUA, LI LIMING: "Research Progress of DNA Methylation Age", JOURNAL OF PREVENTIVE MEDICINE INFORMATION, vol. 36, no. 7, 28 July 2020 (2020-07-28), pages 896 - 902, XP093109373
GAO X ET AL: "Immunomodulatory activity of resveratrol: suppression of lymphocyte proliferation, development of cell-mediated cytotoxicity, and cytokine production.", BIOCHEMICAL PHARMACOLOGY, ELSEVIER, US, vol. 62, 1 November 2001 (2001-11-01), US , pages 1299 - 1308, XP002197358, ISSN: 0006-2952, DOI: 10.1016/S0006-2952(01)00775-4
XIN LI: "Progress in the Study of the Correlation Between DNA Methylation and Individual Age", THE JOURNAL OF LAW & MEDICINE, vol. 13, no. 4, 20 November 2006 (2006-11-20), pages 284 - 288, XP093109369
HEXIANG PENG, GAO WENJING, CAO WEIHUA, LI LIMING: "Research Progress of DNA Methylation Age", JOURNAL OF PREVENTIVE MEDICINE INFORMATION, vol. 36, no. 7, 28 July 2020 (2020-07-28), pages 896 - 902, XP093109373
GAO X ET AL: "Immunomodulatory activity of resveratrol: suppression of lymphocyte proliferation, development of cell-mediated cytotoxicity, and cytokine production.", BIOCHEMICAL PHARMACOLOGY, ELSEVIER, US, vol. 62, 1 November 2001 (2001-11-01), US , pages 1299 - 1308, XP002197358, ISSN: 0006-2952, DOI: 10.1016/S0006-2952(01)00775-4
Attorney, Agent or Firm:
SHANGHAI SHENXIN LAW FIRM (CN)
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