Title:
BIOMOLECULE ANALYSIS METHOD, BIOMOLECULE ANALYZING REAGENT, AND BIOMOLECULE ANALYSIS DEVICE
Document Type and Number:
WIPO Patent Application WO/2022/264243
Kind Code:
A1
Abstract:
This biomolecule analysis method is characterized by including: preparing a biomolecule analysis device provided with a thin film having nanopores with diameters in the range of ±20% of the diameters of biomolecules, a first liquid tank and a second liquid tank separated by the thin film, a first electrode disposed in the first liquid tank, a second electrode disposed in the second liquid tank, and a biopolymer degradation mechanism that degrades a biopolymer into the biomolecules; degrading the biopolymer into the biomolecules in the biopolymer degradation mechanism; and applying a voltage between the first electrode and the second electrode, under conditions where a measurement solution is enclosed in the first liquid tank and the second liquid tank, to measure a current flowing between the first electrode and the second electrode. The biomolecule analysis method is also characterized in that said measurement liquid comprises ammonium ions and sulfate ions.
Inventors:
AKAHORI RENA (JP)
NAKAGAWA TATSUO (JP)
NGUYENPHAM HAI HUY (JP)
NAKAGAWA TATSUO (JP)
NGUYENPHAM HAI HUY (JP)
Application Number:
PCT/JP2021/022614
Publication Date:
December 22, 2022
Filing Date:
June 15, 2021
Export Citation:
Assignee:
HITACHI HIGH TECH CORP (JP)
International Classes:
C12M1/42; C12Q1/6869; G01N27/02
Domestic Patent References:
| WO2020084705A1 | 2020-04-30 |
Other References:
GOTO YUSUKE, MATSUI KAZUMA, YANAGI ITARU, TAKEDA KEN-ICHI: "Silicon nitride nanopore created by dielectric breakdown with a divalent cation: deceleration of translocation speed and identification of single nucleotides", NANOSCALE, ROYAL SOCIETY OF CHEMISTRY, UNITED KINGDOM, vol. 11, no. 30, 1 August 2019 (2019-08-01), United Kingdom , pages 14426 - 14433, XP093017665, ISSN: 2040-3364, DOI: 10.1039/C9NR03563J
GOTO, Y. ET AL.: "Solid-state nanopores towards single-molecule DNA sequencing", JOURNAL OF HUMAN GENETICS., vol. 65, 2020, pages 69 - 77, XP036929931, DOI: 10.1038/s10038-019-0655-8
CLARKE, J. ET AL.: "Continuous base identification for single-molecule nanopore DNA sequencing", NATURE NANOTECHNOLOGY., vol. 4, 2009, pages 265 - 270, XP055486302, DOI: 10.1038/nnano.2009.12
AYUB, M. ET AL.: "Nanopore-Based Identification of Individual Nucleotides for Direct RNA Sequencing", NANO LETTERS., vol. 13, 2013, pages 6144 - 6150, XP055167658, DOI: 10.1021/nl403469r
GOTO, Y. ET AL.: "Solid-state nanopores towards single-molecule DNA sequencing", JOURNAL OF HUMAN GENETICS., vol. 65, 2020, pages 69 - 77, XP036929931, DOI: 10.1038/s10038-019-0655-8
CLARKE, J. ET AL.: "Continuous base identification for single-molecule nanopore DNA sequencing", NATURE NANOTECHNOLOGY., vol. 4, 2009, pages 265 - 270, XP055486302, DOI: 10.1038/nnano.2009.12
AYUB, M. ET AL.: "Nanopore-Based Identification of Individual Nucleotides for Direct RNA Sequencing", NANO LETTERS., vol. 13, 2013, pages 6144 - 6150, XP055167658, DOI: 10.1021/nl403469r
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
Download PDF:
Previous Patent: LIQUID DROPLET DISCHARGING DEVICE AND LIQUID DROPLET DISCHARGING METHOD
Next Patent: TOBACCO PRODUCT
Next Patent: TOBACCO PRODUCT