Title:
CAPPING ENZYME OF FLAVIVIRUS AND UTILIZATION OF THIS PROTEIN IN A PROCESS TO TEST DRUGS WITH ANTIVIRAL PROPERTIES
Document Type and Number:
WIPO Patent Application WO2002097075
Kind Code:
A3
Abstract:
The present invention concerns an isolated and purified polypeptide capable of acting as a guanylyltransferase and methyltransferase comprising capping enzyme of flavivirus (CEF). The present invention also concerns the use of such polypeptide to identify biologically active molecule which can be used in the treatment or the prevention of diseases resulting from Flavivirus infection.
Inventors:
ROMETTE JEAN-LOUIS (FR)
BENARROCHE DELPHINE (FR)
CANARD BRUNO (FR)
EGLOFF MARIE-PIERRE (FR)
BENARROCHE DELPHINE (FR)
CANARD BRUNO (FR)
EGLOFF MARIE-PIERRE (FR)
Application Number:
PCT/IB2002/002908
Publication Date:
October 09, 2003
Filing Date:
May 31, 2002
Export Citation:
Assignee:
CENTRE NAT RECH SCIENT (FR)
ROMETTE JEAN-LOUIS (FR)
BENARROCHE DELPHINE (FR)
CANARD BRUNO (FR)
EGLOFF MARIE-PIERRE (FR)
ROMETTE JEAN-LOUIS (FR)
BENARROCHE DELPHINE (FR)
CANARD BRUNO (FR)
EGLOFF MARIE-PIERRE (FR)
International Classes:
A61K31/675; A61P31/14; C07K16/40; C12N1/15; G01N33/50; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12N9/12; C12N15/09; C12P21/08; C12Q1/48; G01N33/15; G01N33/60; (IPC1-7): C12N9/12; C12N15/54; G01N33/573; A61K31/52
Foreign References:
| US6015573A | 2000-01-18 |
Other References:
DATABASE EMBL [online] 1 November 1999 (1999-11-01), "Dengue virus type 2 polyprotein (3291aa)", XP002230107, retrieved from EBI Database accession no. Q9WLZ4
DATABASE EMBL [online] 1 January 1988 (1988-01-01), "genome polyprotein of West Nile virus (3430aa)", XP002230108, retrieved from EBI Database accession no. P06935
DATABASE EMBL [online] 1 November 1999 (1999-11-01), "genome polyprotein fragment of Yellow fever virus (905aa)", XP002230109, retrieved from EBI Database accession no. Q9W9I1
TAN B H ET AL: "Recombinant dengue type 1 virus NS5 protein expressed in Escherichia coli exhibits RNA-dependent RNA polymerase activity.", VIROLOGY. UNITED STATES 15 FEB 1996, vol. 216, no. 2, 15 February 1996 (1996-02-15), pages 317 - 325, XP002230101, ISSN: 0042-6822
KOONIN EUGENE V: "Computer-assisted identification of a putative methyltransferase domain in NS5 protein of flaviviruses and lambda-2 protein of reovirus.", JOURNAL OF GENERAL VIROLOGY, vol. 74, no. 4, 1993, pages 733 - 740, XP009005088, ISSN: 0022-1317
HAKANSSON K ET AL: "X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes.", CELL. UNITED STATES 16 MAY 1997, vol. 89, no. 4, 16 May 1997 (1997-05-16), pages 545 - 553, XP002230102, ISSN: 0092-8674
SCHLUCKEBIER GERD ET AL: "Universal catalytic domain structure of AdoMet-dependent methyltransferases.", JOURNAL OF MOLECULAR BIOLOGY, vol. 247, no. 1, 1995, pages 16 - 20, XP002230103, ISSN: 0022-2836
NILES E G ET AL: "Direct photolinkage of GTP to the vaccinia virus mRNA (guanine-7-) methyltransferase GTP methyl acceptor site.", BIOCHEMISTRY. UNITED STATES 23 AUG 1994, vol. 33, no. 33, 23 August 1994 (1994-08-23), pages 9898 - 9903, XP001122058, ISSN: 0006-2960
JORDAN INGO ET AL: "Ribavirin inhibits West Nile virus replication and cytopathic effect in neural cells.", JOURNAL OF INFECTIOUS DISEASES, vol. 182, no. 4, October 2000 (2000-10-01), pages 1214 - 1217, XP002230104, ISSN: 0022-1899
LAMPIO ANJA ET AL: "Guanosine nucleotide analogs as inhibitors of alphavirus mRNA capping enzyme.", ANTIVIRAL RESEARCH, vol. 42, no. 1, May 1999 (1999-05-01), pages 35 - 46, XP002230105, ISSN: 0166-3542
LEYSSEN P ET AL: "PERSPECTIVES FOR THE TREATMENT OF INFECTIONS WITH FLAVIVIRIDAE", CLINICAL MICROBIOLOGY REVIEWS, WASHINGTON, DC, US, vol. 13, no. 1, January 2000 (2000-01-01), pages 67 - 82, XP000889854, ISSN: 0893-8512
EGLOFF MARIE-PIERRE ET AL: "An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: Crystal structure and functional characterization.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 21, no. 11, 3 June 2002 (2002-06-03), June 3, 2002, pages 2757 - 2768, XP002230106, ISSN: 0261-4189
DATABASE EMBL [online] 1 January 1988 (1988-01-01), "genome polyprotein of West Nile virus (3430aa)", XP002230108, retrieved from EBI Database accession no. P06935
DATABASE EMBL [online] 1 November 1999 (1999-11-01), "genome polyprotein fragment of Yellow fever virus (905aa)", XP002230109, retrieved from EBI Database accession no. Q9W9I1
TAN B H ET AL: "Recombinant dengue type 1 virus NS5 protein expressed in Escherichia coli exhibits RNA-dependent RNA polymerase activity.", VIROLOGY. UNITED STATES 15 FEB 1996, vol. 216, no. 2, 15 February 1996 (1996-02-15), pages 317 - 325, XP002230101, ISSN: 0042-6822
KOONIN EUGENE V: "Computer-assisted identification of a putative methyltransferase domain in NS5 protein of flaviviruses and lambda-2 protein of reovirus.", JOURNAL OF GENERAL VIROLOGY, vol. 74, no. 4, 1993, pages 733 - 740, XP009005088, ISSN: 0022-1317
HAKANSSON K ET AL: "X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes.", CELL. UNITED STATES 16 MAY 1997, vol. 89, no. 4, 16 May 1997 (1997-05-16), pages 545 - 553, XP002230102, ISSN: 0092-8674
SCHLUCKEBIER GERD ET AL: "Universal catalytic domain structure of AdoMet-dependent methyltransferases.", JOURNAL OF MOLECULAR BIOLOGY, vol. 247, no. 1, 1995, pages 16 - 20, XP002230103, ISSN: 0022-2836
NILES E G ET AL: "Direct photolinkage of GTP to the vaccinia virus mRNA (guanine-7-) methyltransferase GTP methyl acceptor site.", BIOCHEMISTRY. UNITED STATES 23 AUG 1994, vol. 33, no. 33, 23 August 1994 (1994-08-23), pages 9898 - 9903, XP001122058, ISSN: 0006-2960
JORDAN INGO ET AL: "Ribavirin inhibits West Nile virus replication and cytopathic effect in neural cells.", JOURNAL OF INFECTIOUS DISEASES, vol. 182, no. 4, October 2000 (2000-10-01), pages 1214 - 1217, XP002230104, ISSN: 0022-1899
LAMPIO ANJA ET AL: "Guanosine nucleotide analogs as inhibitors of alphavirus mRNA capping enzyme.", ANTIVIRAL RESEARCH, vol. 42, no. 1, May 1999 (1999-05-01), pages 35 - 46, XP002230105, ISSN: 0166-3542
LEYSSEN P ET AL: "PERSPECTIVES FOR THE TREATMENT OF INFECTIONS WITH FLAVIVIRIDAE", CLINICAL MICROBIOLOGY REVIEWS, WASHINGTON, DC, US, vol. 13, no. 1, January 2000 (2000-01-01), pages 67 - 82, XP000889854, ISSN: 0893-8512
EGLOFF MARIE-PIERRE ET AL: "An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: Crystal structure and functional characterization.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 21, no. 11, 3 June 2002 (2002-06-03), June 3, 2002, pages 2757 - 2768, XP002230106, ISSN: 0261-4189
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