Title:
CARBON MONOXIDE DEHYDROGENASE HAVING EXCELLENT AFFINITY AND REACTION RATE FOR ELECTRON CARRIER, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/019371
Kind Code:
A1
Abstract:
One aspect relates to a carbon monoxide dehydrogenase having excellent affinity and/or reaction rate for an electron carrier, and, particularly, provides a mutant carbon monoxide dehydrogenase (CO dehydrogenase) of which amino acid residues are mutated so that the affinity and/or reaction rate for an electron carrier is increased. The carbon monoxide dehydrogenase (CO dehydrogenase) can detoxify carbon monoxide by rapidly oxidizing and converting, to carbon dioxide, carbon monoxide which is toxic at room temperature and normal pressure. Furthermore, the present invention can remove carbon monoxide, which is discharged in large amounts from industries such as the petrochemical and steel industries, cigarette combustion, household cooking appliances, and the combustion of various boilers, through cigarette filters, air purifiers, suction filters of household cooking appliances, gas boilers and the like, and thus is variously usable.
Inventors:
KIM YONG HWAN (KR)
KANG SUNG HEUCK (KR)
KIM SUK MIN (KR)
KANG SUNG HEUCK (KR)
KIM SUK MIN (KR)
Application Number:
PCT/KR2020/011466
Publication Date:
January 27, 2022
Filing Date:
August 27, 2020
Export Citation:
Assignee:
ULSAN NAT INST SCIENCE & TECH UNIST (KR)
International Classes:
C12N9/02; B01D53/84; C12N15/70
Foreign References:
| KR101826207B1 | 2018-02-06 | |||
| KR20170036789A | 2017-04-03 |
Other References:
LEE JINHEE, HO WON HWANG, SUK MIN KIM, AKHTAR JUNAID, YONG HWAN KIM: "In-silico based redesign of CO-dehydrogenase catalyzing the oxidation of toxic waste CO gas for improved O2 resistance and mediator affinity", ENZYME ENGINEERING XXV; POSTER NO. 31, 19 September 2019 (2019-09-19), XP055889409
SPANGLER NATHAN J., MEYERS MONICA R., GIERKE KARIN L., KERBY ROBERT L., ROBERTS GARY P., LUDDEN PAUL W.: "Substitution of Valine for Histidine 265 in Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum Affects Activity and Spectroscopic States", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 273, no. 7, 1 February 1998 (1998-02-01), US , pages 4059 - 4064, XP055889411, ISSN: 0021-9258, DOI: 10.1074/jbc.273.7.4059
WITTENBORN ELIZABETH C., GUENDON CHLOÉ, MERROUCH MÉRIEM, BENVENUTI MARTINO, FOURMOND VINCENT, LÉGER CHRISTOPHE, DRENNAN CATHERINE : "The Solvent-Exposed Fe–S D-Cluster Contributes to Oxygen-Resistance in Desulfovibrio vulgaris Ni–Fe Carbon Monoxide Dehydrogenase", ACS CATALYSIS, AMERICAN CHEMICAL SOCIETY, US, vol. 10, no. 13, 2 July 2020 (2020-07-02), US , pages 7328 - 7335, XP055889413, ISSN: 2155-5435, DOI: 10.1021/acscatal.0c00934
SPANGLER NATHAN J., MEYERS MONICA R., GIERKE KARIN L., KERBY ROBERT L., ROBERTS GARY P., LUDDEN PAUL W.: "Substitution of Valine for Histidine 265 in Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum Affects Activity and Spectroscopic States", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 273, no. 7, 1 February 1998 (1998-02-01), US , pages 4059 - 4064, XP055889411, ISSN: 0021-9258, DOI: 10.1074/jbc.273.7.4059
WITTENBORN ELIZABETH C., GUENDON CHLOÉ, MERROUCH MÉRIEM, BENVENUTI MARTINO, FOURMOND VINCENT, LÉGER CHRISTOPHE, DRENNAN CATHERINE : "The Solvent-Exposed Fe–S D-Cluster Contributes to Oxygen-Resistance in Desulfovibrio vulgaris Ni–Fe Carbon Monoxide Dehydrogenase", ACS CATALYSIS, AMERICAN CHEMICAL SOCIETY, US, vol. 10, no. 13, 2 July 2020 (2020-07-02), US , pages 7328 - 7335, XP055889413, ISSN: 2155-5435, DOI: 10.1021/acscatal.0c00934
Attorney, Agent or Firm:
Y.P.LEE, MOCK & PARTNERS (KR)
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