Title:
CARBOXYL MAGNETIC BEAD COUPLING-MODIFIED NUCLEIC ACID PROBE, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/077560
Kind Code:
A1
Abstract:
The present invention provides a carboxyl magnetic bead coupling-modified nucleic acid probe, a preparation method therefor and an application thereof. The preparation method comprises: activating carboxyl magnetic beads to obtain activated carboxyl magnetic beads; and co-incubating the activated carboxyl magnetic beads and an amino-modified nucleic acid probe in a coupling reaction solution to obtain the carboxyl magnetic bead coupling-modified nucleic acid probe. The coupling reaction solution is a mixed solution of morpholine ethanesulfonic acid and N,N-dimethyl acetamide. The carboxyl magnetic bead coupling-modified nucleic acid probe is used for targeted capture of target nucleic acid in a biological sample. The nucleic acid obtained by targeted capture can satisfy requirements of gene mutation detection and gene methylation detection, and can avoid influence of a background gene and an inhibitor in the biological sample with high capture efficiency. The present invention can perform targeted capture of specific genes in low-abundance DNA/RNA samples such as blood, urine, and feces.
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Inventors:
CHENG XIAOWEI (CN)
LI TING (CN)
TIAN TIAN (CN)
BA ZHAOFEN (CN)
ZHANG TIANTIAN (CN)
LI TING (CN)
TIAN TIAN (CN)
BA ZHAOFEN (CN)
ZHANG TIANTIAN (CN)
Application Number:
PCT/CN2019/123434
Publication Date:
April 29, 2021
Filing Date:
December 05, 2019
Export Citation:
Assignee:
JIANGSU MICRODIAG BIOMEDICINE TECH CO LTD (CN)
International Classes:
C12Q1/68; C12N15/11
Domestic Patent References:
| WO2011045394A1 | 2011-04-21 |
Foreign References:
| CN108396059A | 2018-08-14 | |||
| CN109781871A | 2019-05-21 |
Attorney, Agent or Firm:
CO-HORIZON INTELLECTUAL PROPERTY INC. (CN)
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