Title:
CARS MICROSCOPE
Document Type and Number:
WIPO Patent Application WO/2014/061147
Kind Code:
A1
Abstract:
Conventional microscopes that measure the distribution of species have difficulty in measuring the refractive index distribution of a sample and are limited in the information that can be obtained.
A CARS microscope according to the present invention is configured to comprise: a first light splitting means (102) for splitting light from a light source (110) into a first pump beam and a second pump beam; a Stokes light source (104) into which the second pump beam is input and that outputs a Stokes beam; a combining means (108) for generating a combined beam by combining the first pump beam and the Stokes beam; a first focusing means (109) for focusing the combined beam into a sample (110); a first detector (113) for detecting CARS light that has a wavelength different from the combined beam and that is generated from within the sample; a second light splitting means (107) for branching, as a reference beam, a portion of a beam of at least one of the second pump beam and the Stokes beam; a second combining means (017) for generating interfering light by combining the beam from within the sample and the reference beam; and a second detector for detecting the interfering light.
Inventors:
MIKAMI HIDEHARU (JP)
Application Number:
PCT/JP2012/077030
Publication Date:
April 24, 2014
Filing Date:
October 19, 2012
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
G01N21/65; G01N21/17; G02B21/00
Foreign References:
| JP2010139327A | 2010-06-24 | |||
| JP2004534232A | 2004-11-11 | |||
| JP2012018122A | 2012-01-26 | |||
| JPH0445140B2 | 1992-07-23 |
Other References:
BREDFELDT J S ET AL.: "Molecularly sensitive optical coherence tomography", OPTICS LETTERS, vol. 30, no. 5, 1 March 2005 (2005-03-01), pages 495 - 497, XP055215171
SARUNIC M V ET AL.: "Spectral domain second- harmonic optical coherence tomography", OPTICS LETTERS, vol. 30, no. 18, 15 September 2005 (2005-09-15), pages 2391 - 2393, XP055215036
VINEGONI C ET AL.: "Nonlinear optical contrast enhancement for optical coherence tomography", OPTICS EXPRESS, vol. 12, no. 2, 26 January 2004 (2004-01-26), pages 331 - 341, XP055253307
LABRUYERE A ET AL.: "Compact supercontinuum sources and their biomedical applications", OPTICAL FIBER TECHNOLOGY, vol. 18, no. 5, September 2012 (2012-09-01), pages 375 - 378, XP028944538
MITSUO GOMI ET AL.: "A Remote Laser-Probing System for Combustion Measurements", TECHNICAL REPORT OF NATIONAL AEROSPACE LABORATORY, June 1988 (1988-06-01), pages 1 - 18, XP008179171
W. DENK ET AL.: "Two-Photon Laser Scanning Fluorescence Microscopy", SCIENCE, vol. 248, no. 4951, 1990, pages 73 - 76, XP000381741, DOI: doi:10.1126/science.2321027
P. J. CAMPAGNOLA ET AL.: "Second-harmonic imaging microscopy for visualizing biomoleeuiar arrays in cells, tissues and organisms", NATURE BIOTECHNOLOGY, vol. 21, 2003, pages 1356 - 1360
M. OKUNO ET AL.: "Quantitative CARS Molecular finger printing of living Cells", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 49, 2010, pages 6773 - 6777
B.G. SAAR ET AL.: "Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering", SCIENCE, vol. 330, 2010, pages 1368, XP055053264, DOI: doi:10.1126/science.1197236
J. P. R. DAY; K. F. DOMKE; G, RAGO; H. KANO; H.HAMAGUCHI; E. M. VARTIAINEN; M. BONN: "Quantitative Coherent Anti-Stokes Scattering (CARS) Microscopy", J, PHYS.CHEM. B, vol. 115, 2011, pages 7713 - 7725, XP055017866, DOI: doi:10.1021/jp200606e
M. WOJTKOWSKI: "Full range complex spectral optical coherence tomography technique in eye imaging", OPTICS LETTERS, vol. 27, no. 16, 2002, pages 1415 - 1417, XP002513622, DOI: doi:10.1364/OL.27.001415
S, BEGIN ET AL.: "Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system", BIOMEDICAL OPTICS EXPRESS, vol. 2, no. 5, 2011, pages 1296 - 1306
Y. YASUNO ET AL.: "Three-dimensional and high-speed swept-source optical coherence tomography for in vivo investigation of human anterior eye segments", OPTICS EXPRESS, vol. 13, no. 26, 2005, pages 10652 - 10664
SARUNIC M V ET AL.: "Spectral domain second- harmonic optical coherence tomography", OPTICS LETTERS, vol. 30, no. 18, 15 September 2005 (2005-09-15), pages 2391 - 2393, XP055215036
VINEGONI C ET AL.: "Nonlinear optical contrast enhancement for optical coherence tomography", OPTICS EXPRESS, vol. 12, no. 2, 26 January 2004 (2004-01-26), pages 331 - 341, XP055253307
LABRUYERE A ET AL.: "Compact supercontinuum sources and their biomedical applications", OPTICAL FIBER TECHNOLOGY, vol. 18, no. 5, September 2012 (2012-09-01), pages 375 - 378, XP028944538
MITSUO GOMI ET AL.: "A Remote Laser-Probing System for Combustion Measurements", TECHNICAL REPORT OF NATIONAL AEROSPACE LABORATORY, June 1988 (1988-06-01), pages 1 - 18, XP008179171
W. DENK ET AL.: "Two-Photon Laser Scanning Fluorescence Microscopy", SCIENCE, vol. 248, no. 4951, 1990, pages 73 - 76, XP000381741, DOI: doi:10.1126/science.2321027
P. J. CAMPAGNOLA ET AL.: "Second-harmonic imaging microscopy for visualizing biomoleeuiar arrays in cells, tissues and organisms", NATURE BIOTECHNOLOGY, vol. 21, 2003, pages 1356 - 1360
M. OKUNO ET AL.: "Quantitative CARS Molecular finger printing of living Cells", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 49, 2010, pages 6773 - 6777
B.G. SAAR ET AL.: "Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering", SCIENCE, vol. 330, 2010, pages 1368, XP055053264, DOI: doi:10.1126/science.1197236
J. P. R. DAY; K. F. DOMKE; G, RAGO; H. KANO; H.HAMAGUCHI; E. M. VARTIAINEN; M. BONN: "Quantitative Coherent Anti-Stokes Scattering (CARS) Microscopy", J, PHYS.CHEM. B, vol. 115, 2011, pages 7713 - 7725, XP055017866, DOI: doi:10.1021/jp200606e
M. WOJTKOWSKI: "Full range complex spectral optical coherence tomography technique in eye imaging", OPTICS LETTERS, vol. 27, no. 16, 2002, pages 1415 - 1417, XP002513622, DOI: doi:10.1364/OL.27.001415
S, BEGIN ET AL.: "Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system", BIOMEDICAL OPTICS EXPRESS, vol. 2, no. 5, 2011, pages 1296 - 1306
Y. YASUNO ET AL.: "Three-dimensional and high-speed swept-source optical coherence tomography for in vivo investigation of human anterior eye segments", OPTICS EXPRESS, vol. 13, no. 26, 2005, pages 10652 - 10664
Attorney, Agent or Firm:
INOUE Manabu et al. (JP)
Manabu Inoue (JP)
Manabu Inoue (JP)
Download PDF:
Previous Patent: GENE ANALYSIS METHOD, AND DEVICE AND KIT FOR ANALYSIS OF GENE
Next Patent: SLAVE STATION TERMINAL
Next Patent: SLAVE STATION TERMINAL