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Title:
CARS MICROSCOPE
Document Type and Number:
WIPO Patent Application WO/2014/061147
Kind Code:
A1
Abstract:
Conventional microscopes that measure the distribution of species have difficulty in measuring the refractive index distribution of a sample and are limited in the information that can be obtained. A CARS microscope according to the present invention is configured to comprise: a first light splitting means (102) for splitting light from a light source (110) into a first pump beam and a second pump beam; a Stokes light source (104) into which the second pump beam is input and that outputs a Stokes beam; a combining means (108) for generating a combined beam by combining the first pump beam and the Stokes beam; a first focusing means (109) for focusing the combined beam into a sample (110); a first detector (113) for detecting CARS light that has a wavelength different from the combined beam and that is generated from within the sample; a second light splitting means (107) for branching, as a reference beam, a portion of a beam of at least one of the second pump beam and the Stokes beam; a second combining means (017) for generating interfering light by combining the beam from within the sample and the reference beam; and a second detector for detecting the interfering light.

Inventors:
MIKAMI HIDEHARU (JP)
Application Number:
PCT/JP2012/077030
Publication Date:
April 24, 2014
Filing Date:
October 19, 2012
Export Citation:
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Assignee:
HITACHI LTD (JP)
International Classes:
G01N21/65; G01N21/17; G02B21/00
Foreign References:
JP2010139327A2010-06-24
JP2004534232A2004-11-11
JP2012018122A2012-01-26
JPH0445140B21992-07-23
Other References:
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LABRUYERE A ET AL.: "Compact supercontinuum sources and their biomedical applications", OPTICAL FIBER TECHNOLOGY, vol. 18, no. 5, September 2012 (2012-09-01), pages 375 - 378, XP028944538
MITSUO GOMI ET AL.: "A Remote Laser-Probing System for Combustion Measurements", TECHNICAL REPORT OF NATIONAL AEROSPACE LABORATORY, June 1988 (1988-06-01), pages 1 - 18, XP008179171
See also references of EP 2910928A4
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M. OKUNO ET AL.: "Quantitative CARS Molecular finger printing of living Cells", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 49, 2010, pages 6773 - 6777
B.G. SAAR ET AL.: "Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering", SCIENCE, vol. 330, 2010, pages 1368, XP055053264, DOI: doi:10.1126/science.1197236
J. P. R. DAY; K. F. DOMKE; G, RAGO; H. KANO; H.HAMAGUCHI; E. M. VARTIAINEN; M. BONN: "Quantitative Coherent Anti-Stokes Scattering (CARS) Microscopy", J, PHYS.CHEM. B, vol. 115, 2011, pages 7713 - 7725, XP055017866, DOI: doi:10.1021/jp200606e
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S, BEGIN ET AL.: "Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system", BIOMEDICAL OPTICS EXPRESS, vol. 2, no. 5, 2011, pages 1296 - 1306
Y. YASUNO ET AL.: "Three-dimensional and high-speed swept-source optical coherence tomography for in vivo investigation of human anterior eye segments", OPTICS EXPRESS, vol. 13, no. 26, 2005, pages 10652 - 10664
Attorney, Agent or Firm:
INOUE Manabu et al. (JP)
Manabu Inoue (JP)
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