Title:
CELL CULTURE MEDIUM AND CULTURE METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2015/080047
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a cell culture medium, particularly a serum-free cell culture medium, that makes it possible to raise cell growth efficiency without using feeder cells. The present invention provides a serum-free cell culture medium containing growth arrest-specific 6 (GAS6).
Inventors:
KATO TOMOHISA (JP)
KANEMURA YONEHIRO (JP)
SHOFUDA TOMOKO (JP)
FUKUSUMI HAYATO (JP)
KANEMURA YONEHIRO (JP)
SHOFUDA TOMOKO (JP)
FUKUSUMI HAYATO (JP)
Application Number:
PCT/JP2014/080904
Publication Date:
June 04, 2015
Filing Date:
November 21, 2014
Export Citation:
Assignee:
KANEKA CORP (JP)
NAT HOSPITAL ORGANIZATION (JP)
NAT HOSPITAL ORGANIZATION (JP)
International Classes:
C12N5/10; C07K14/47
Domestic Patent References:
| WO2014208295A1 | 2014-12-31 | |||
| WO1999020741A1 | 1999-04-29 |
Foreign References:
| JP2009501014A | 2009-01-15 | |||
| JP2005532805A | 2005-11-04 | |||
| JP2009077716A | 2009-04-16 | |||
| JP2008092811A | 2008-04-24 | |||
| JP2008092882A | 2008-04-24 | |||
| JP2010148391A | 2010-07-08 | |||
| KR20100130677A | 2010-12-14 |
Other References:
CHIN A.C. ET AL.: "Identification of proteins from feeder conditioned medium that support human embryonic stem cells", J. BIOTECHNOL., vol. 130, no. 3, 30 June 2007 (2007-06-30), pages 320 - 328, XP022119696
TOTONCHI M. ET AL.: "Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells", INT. J. DEV. BIOL., vol. 54, no. 5, 2010, pages 877 - 886, XP055074858
HUTCHISON M.R. ET AL.: "SCF, BDNF, and Gas6 are regulators of growth plate chondrocyte proliferation and differentiation", MOL. ENDOCRINOL., vol. 24, no. 1, January 2010 (2010-01-01), pages 193 - 203, XP055346685
LOESER R.F. ET AL.: "Human chondrocyte expression of growth-arrest-specific gene 6 and the tyrosine kinase receptor axl: potential role in autocrine signaling in cartilage", ARTHRITIS. RHEUM., vol. 40, no. 8, August 1997 (1997-08-01), pages 1455 - 1465, XP055124158
XIONG W. ET AL.: "Gas6 and the Tyro 3 receptor tyrosine kinase subfamily regulate the phagocytic function of Sertoli cells", REPRODUCTION, vol. 135, no. 1, January 2008 (2008-01-01), pages 77 - 87, XP055346692
BELLIDO-MARTIN L. ET AL.: "Vitamin K-dependent actions of Gas6", VITAM. HORM., vol. 78, 2008, pages 185 - 209, XP029137001
HOVATTA O; MIKKOLA M; GERTOW K ET AL.: "A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells", HUM REPROD, vol. 18, 2003, pages 1404 - 1409
RICHARDS M; FONG CY; CHAN WK ET AL.: "Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells", NAT BIOTECHNOL, vol. 20, 2002, pages 933 - 936
CHENG L; HAMMOND H; YE Z ET AL.: "Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture", STEM CELLS, vol. 21, 2003, pages 131 - 142
RICHARDS M; TAN S; FONG CY ET AL.: "Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells", STEM CELLS, vol. 21, 2003, pages 546 - 556
YUE X-S; FUJISHIRO M; NISHIOKA C; ARAI T; TAKAHASHI E; GONG J-S; AKAIKE T; ITO Y: "Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation", PLOS ONE, vol. 7, 2012, pages E32707
HAYATO FUKUSUMI; YONEHIRO KANEMURA: "Development of feeder cell-free culture technique of human ES/iPS cells", IGAKU NO AYUMI, vol. 239, 2011, pages 1338 - 1344
WANG L; SCHULZ TC; SHERRER ES; DAUPHIN DS; SHIN S ET AL.: "Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling", BLOOD, vol. 110, 2007, pages 4111 - 4119
VERONIKA A; PETER WA; STEPHEN B; NISSIM B; JENNIFER B ET AL.: "Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells", IN VITRO CELL DEV BIOL ANIM, vol. 46, 2010, pages 247 - 58
CHEN G; GULBRANSON DR; HOU Z; BOLIN JM; RUOTTI V; PROBASCO MD ET AL.: "Chemically defined conditions for human iPSC derivation and culture", NAT METHODS, vol. 8, 2011, pages 424 - 429
CHIN AC; FONG WJ; GOH LT; PHILP R; OH SK; CHOO AB: "Identification of proteins from feeder conditioned medium that support human embryonic stem cells.", JOURNAL OF BIOTECHNOLOGY, vol. 130, 2007, pages 320 - 8
MANTON KJ; RICHARDS S; VAN LONKHUYZEN D; CORMACK L; LEAVESLEY D; UPTON Z: "A chimeric vitronectin: IGF-I protein supports feeder-cell-free and serum-free culture of human embryonic stem cells", STEM CELLS DEV, vol. 19, no. 9, 2010, pages 1297 - 1305
JUNICHI SHIMODA; TAKAYOSHI HAMAMOTO: "Gas6-deficient mice", JAPANESE JOURNAL OF THROMBOSIS AND HEMOSTASIS, vol. 12, no. 6, 2001, pages 514 - 521
VYACHESLAV AK.: "Axl-dependent signaling: a clinical update", CLINICAL SCIENCE, vol. 122, 2012, pages 361 - 368
EG CELLS: PROC NATL ACAD SCI USA., vol. 95, 1998, pages 13726 - 13731
NATURE, vol. 456, 2008, pages 344 - 349
See also references of EP 3075846A4
TOTONCHI M. ET AL.: "Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells", INT. J. DEV. BIOL., vol. 54, no. 5, 2010, pages 877 - 886, XP055074858
HUTCHISON M.R. ET AL.: "SCF, BDNF, and Gas6 are regulators of growth plate chondrocyte proliferation and differentiation", MOL. ENDOCRINOL., vol. 24, no. 1, January 2010 (2010-01-01), pages 193 - 203, XP055346685
LOESER R.F. ET AL.: "Human chondrocyte expression of growth-arrest-specific gene 6 and the tyrosine kinase receptor axl: potential role in autocrine signaling in cartilage", ARTHRITIS. RHEUM., vol. 40, no. 8, August 1997 (1997-08-01), pages 1455 - 1465, XP055124158
XIONG W. ET AL.: "Gas6 and the Tyro 3 receptor tyrosine kinase subfamily regulate the phagocytic function of Sertoli cells", REPRODUCTION, vol. 135, no. 1, January 2008 (2008-01-01), pages 77 - 87, XP055346692
BELLIDO-MARTIN L. ET AL.: "Vitamin K-dependent actions of Gas6", VITAM. HORM., vol. 78, 2008, pages 185 - 209, XP029137001
HOVATTA O; MIKKOLA M; GERTOW K ET AL.: "A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells", HUM REPROD, vol. 18, 2003, pages 1404 - 1409
RICHARDS M; FONG CY; CHAN WK ET AL.: "Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells", NAT BIOTECHNOL, vol. 20, 2002, pages 933 - 936
CHENG L; HAMMOND H; YE Z ET AL.: "Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture", STEM CELLS, vol. 21, 2003, pages 131 - 142
RICHARDS M; TAN S; FONG CY ET AL.: "Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells", STEM CELLS, vol. 21, 2003, pages 546 - 556
YUE X-S; FUJISHIRO M; NISHIOKA C; ARAI T; TAKAHASHI E; GONG J-S; AKAIKE T; ITO Y: "Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation", PLOS ONE, vol. 7, 2012, pages E32707
HAYATO FUKUSUMI; YONEHIRO KANEMURA: "Development of feeder cell-free culture technique of human ES/iPS cells", IGAKU NO AYUMI, vol. 239, 2011, pages 1338 - 1344
WANG L; SCHULZ TC; SHERRER ES; DAUPHIN DS; SHIN S ET AL.: "Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling", BLOOD, vol. 110, 2007, pages 4111 - 4119
VERONIKA A; PETER WA; STEPHEN B; NISSIM B; JENNIFER B ET AL.: "Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells", IN VITRO CELL DEV BIOL ANIM, vol. 46, 2010, pages 247 - 58
CHEN G; GULBRANSON DR; HOU Z; BOLIN JM; RUOTTI V; PROBASCO MD ET AL.: "Chemically defined conditions for human iPSC derivation and culture", NAT METHODS, vol. 8, 2011, pages 424 - 429
CHIN AC; FONG WJ; GOH LT; PHILP R; OH SK; CHOO AB: "Identification of proteins from feeder conditioned medium that support human embryonic stem cells.", JOURNAL OF BIOTECHNOLOGY, vol. 130, 2007, pages 320 - 8
MANTON KJ; RICHARDS S; VAN LONKHUYZEN D; CORMACK L; LEAVESLEY D; UPTON Z: "A chimeric vitronectin: IGF-I protein supports feeder-cell-free and serum-free culture of human embryonic stem cells", STEM CELLS DEV, vol. 19, no. 9, 2010, pages 1297 - 1305
JUNICHI SHIMODA; TAKAYOSHI HAMAMOTO: "Gas6-deficient mice", JAPANESE JOURNAL OF THROMBOSIS AND HEMOSTASIS, vol. 12, no. 6, 2001, pages 514 - 521
VYACHESLAV AK.: "Axl-dependent signaling: a clinical update", CLINICAL SCIENCE, vol. 122, 2012, pages 361 - 368
EG CELLS: PROC NATL ACAD SCI USA., vol. 95, 1998, pages 13726 - 13731
NATURE, vol. 456, 2008, pages 344 - 349
See also references of EP 3075846A4
Attorney, Agent or Firm:
SIKs & Co. (JP)
Patent business corporation patent firm Sykes (JP)
Patent business corporation patent firm Sykes (JP)
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