Title:
CELL CULTURE, METHOD FOR EVALUATING CELL CULTURE, METHOD FOR PRODUCING CELL CULTURE, AND MARKER FOR USE IN EVALUATION OF CHONDROID TISSUE FORMATION PROPERTY
Document Type and Number:
WIPO Patent Application WO/2021/182633
Kind Code:
A1
Abstract:
Provided is a cell culture suitable for use in the repair of a cartilage. The present invention provides a cell culture having a chondroid tissue formation property, the cell culture comprising a cell mass in which the expression level of at least one cell surface marker selected from the group consisting of CD166, CD165, CD99, GD2, STRO-1, CD108, CD164, CD6, CD106 and CD107b is equal to or lower than a threshold value for each of the cell surface markers, and/or the expression level of at least one cell surface marker selected from the group consisting of CD26, CD73, CD105, CD44, CD120a, CD201, EGFR, CD146, CD140a and CD90 is equal to or higher than a threshold value for each of the cell surface markers.
Inventors:
SATO MASATO (JP)
TAKAHASHI TAKUMI (JP)
KAWAGUCHI YUKA (JP)
SATO CHIKAKO (JP)
TSUNODA SATOSHI (JP)
TOHYAMA CHIHARU (JP)
SONG DANDAN (JP)
MIYAZAWA MICHIHIDE (JP)
TAKAHASHI TAKUMI (JP)
KAWAGUCHI YUKA (JP)
SATO CHIKAKO (JP)
TSUNODA SATOSHI (JP)
TOHYAMA CHIHARU (JP)
SONG DANDAN (JP)
MIYAZAWA MICHIHIDE (JP)
Application Number:
PCT/JP2021/010208
Publication Date:
September 16, 2021
Filing Date:
March 12, 2021
Export Citation:
Assignee:
TOKAI UNIV EDUCATIONAL SYSTEM (JP)
CELLSEED INC (JP)
CELLSEED INC (JP)
International Classes:
C12N5/071; A61L27/36; A61L27/38; C07K14/705; C12Q1/04; G01N15/14; G01N33/53
Domestic Patent References:
| WO2018154813A1 | 2018-08-30 | |||
| WO2018154813A1 | 2018-08-30 |
Foreign References:
| US20150259648A1 | 2015-09-17 | |||
| JP2003180819A | 2003-07-02 | |||
| JPH02211865A | 1990-08-23 |
Other References:
TAKAHASHI, TAKUMI ET AL.: "1-7-24 Cell surface marker analysis of polydactyly-derived allogeneic chondrocyte sheet", THE JOURNAL OF JAPANESE ORTHOPAEDIC SURGICAL SOCIETY, vol. 93, no. 8, 9 September 2019 (2019-09-09), JP , pages S1665, XP009539377, ISSN: 0021-5325
MIKI MAEHARA, MASATO SATO, ERIKO TOYODA, TAKUMI TAKAHASHI, ERI OKADA, TOMOMI KOTOKU, MASAHIKO WATANABE: "Characterization of polydactyly-derived chondrocyte sheets versus adult chondrocyte sheets for articular cartilage repair", INFLAMMATION AND REGENERATION, vol. 37, no. 1, 1 December 2017 (2017-12-01), XP055544755, DOI: 10.1186/s41232-017-0053-6
TOYODA, E. ET AL.: "Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue", REGENERATIVE THERAP Y, vol. 10, June 2019 (2019-06-01), pages 17 - 26, XP055856760
WALMSLEY, G. G ET AL.: "High-Throughput Screening of Surface Marker Expression on Undifferentiated and Differentiated Human Adipose-Derived Stromal Cells", TISSUE ENGINEERING:PART A, vol. 21, no. 15, 16, 1 August 2015 (2015-08-01), pages 2281 - 2291, XP055856762
SATO M ET AL.: "Combined surgery and chondrocyte cell-sheet transplantation improves clinical and structural outcomes in knee osteoarthritis.", NPJ REGEN MED, vol. 4, 2019, pages 4
See also references of EP 4119656A4
MIKI MAEHARA, MASATO SATO, ERIKO TOYODA, TAKUMI TAKAHASHI, ERI OKADA, TOMOMI KOTOKU, MASAHIKO WATANABE: "Characterization of polydactyly-derived chondrocyte sheets versus adult chondrocyte sheets for articular cartilage repair", INFLAMMATION AND REGENERATION, vol. 37, no. 1, 1 December 2017 (2017-12-01), XP055544755, DOI: 10.1186/s41232-017-0053-6
TOYODA, E. ET AL.: "Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue", REGENERATIVE THERAP Y, vol. 10, June 2019 (2019-06-01), pages 17 - 26, XP055856760
WALMSLEY, G. G ET AL.: "High-Throughput Screening of Surface Marker Expression on Undifferentiated and Differentiated Human Adipose-Derived Stromal Cells", TISSUE ENGINEERING:PART A, vol. 21, no. 15, 16, 1 August 2015 (2015-08-01), pages 2281 - 2291, XP055856762
SATO M ET AL.: "Combined surgery and chondrocyte cell-sheet transplantation improves clinical and structural outcomes in knee osteoarthritis.", NPJ REGEN MED, vol. 4, 2019, pages 4
See also references of EP 4119656A4
Attorney, Agent or Firm:
WATANABE Kaoru (JP)
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