Title:
CELL CULTURING METHOD USING SMALL-PIECE POROUS MEMBRANE
Document Type and Number:
WIPO Patent Application WO/2021/029409
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a cell culturing method using a small-piece polymer porous membrane. The present invention provides a cell culturing method which does not require stirring and comprises applying cells to a small-piece polymer porous membrane having an area of a surface layer A or B of at most 4 mm2, and culturing the cells. The small-piece polymer porous membrane has a characteristic three‐dimensional structure, and a medium can be more easily supplied and recovered by utilizing the property that small-piece polymer porous membranes are dispersed in a cell culture solution and/or small-piece polymer porous membranes are accumulated in multiple layers, and thus the small-piece polymer porous membranes are dispersed and/or laminated on a bottom part of a cell culture vessel. Consequently, long-term culture and mass culture are achieved, and thus the stable long-term production of exosomes is possible.
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Inventors:
HAGIHARA MASAHIKO (JP)
HIDAKA MOTONOBU (JP)
HARADA TAKASHI (JP)
KONOSHIMA HAYATO (JP)
HIDAKA MOTONOBU (JP)
HARADA TAKASHI (JP)
KONOSHIMA HAYATO (JP)
Application Number:
PCT/JP2020/030614
Publication Date:
February 18, 2021
Filing Date:
August 11, 2020
Export Citation:
Assignee:
UBE INDUSTRIES (JP)
International Classes:
C12M3/00; C12N5/00; C12N5/0775; C12N5/20
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| JP2017517505A | 2017-06-29 | |||
| US20140227766A1 | 2014-08-14 | |||
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Other References:
OGATA ET AL., JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 77, no. 1, 1994, pages 46 - 51
DOSH, R. H. ET AL., SCIENCE REPORTS, vol. 9, 2019
KUROSAWA, H, SEIBUTSU KAGAKU, vol. 91, no. 11, 2013, pages 646 - 653
DOSH, R. H. ET AL., SCIENCE REPORTS, vol. 9, 2019
KUROSAWA, H, SEIBUTSU KAGAKU, vol. 91, no. 11, 2013, pages 646 - 653
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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