Title:
CELL LABELING METHOD
Document Type and Number:
WIPO Patent Application WO/2018/079859
Kind Code:
A1
Abstract:
This cell labeling method is characterized in that a complex, which has acquired membrane affinity as a result of reacting a substrate complex, in which hydrophilic groups are bonded to fluorescent groups, or conjugates of fluorescent groups and hydrophobic groups, with cells having, fixed on the cell membranes, enzymes which catalyze the substrate complex, and removing the hydrophilic groups by way of the catalysis of the enzymes, is diffused on or in the cell membranes of the cells, and made to penetrate the cells.
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Inventors:
KATAYAMA YOSHIKI (JP)
MORI TAKESHI (JP)
MORI TAKESHI (JP)
Application Number:
PCT/JP2017/039644
Publication Date:
May 03, 2018
Filing Date:
October 26, 2017
Export Citation:
Assignee:
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12Q1/02; C07K5/00; G01N21/64; G01N33/53
Foreign References:
| JP2001526380A | 2001-12-18 | |||
| US3074963A | 1963-01-22 | |||
| US2881186A | 1959-04-07 | |||
| CN102286025A | 2011-12-21 | |||
| US20120088720A1 | 2012-04-12 |
Other References:
MUJUMDAR, R. B. ET AL.: "Cyanine dye labeling reagents containing isothiocyanate groups", CYTOMETRY, vol. 10, 1989, pages 11 - 19, XP000034880
RICHARDSON, R. W. ET AL.: "Biotin and fluorescent labeling of RNA using T4 RNA ligase", NUCLEIC ACIDS RESEARCH, vol. 11, no. 18, 1983, pages 6167 - 6184, XP009098117
RICHARDSON, R. W. ET AL.: "Biotin and fluorescent labeling of RNA using T4 RNA ligase", NUCLEIC ACIDS RESEARCH, vol. 11, no. 18, 1983, pages 6167 - 6184, XP009098117
Attorney, Agent or Firm:
KOBAYASHI, Hiroshi et al. (JP)
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