Title:
CELL NICHE ENGINEERING PLATFORM, MULTIPLEXED BIOCHIPS RESULTING THEREFROM AND METHODS OF USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/166965
Kind Code:
A1
Abstract:
Provided are cell niche engineering platform which represents a valuable in vitro tool for investigating physiological and pathological cellular activities, an all-in-one technology to engineer cell niche (particularly soluble cell niche factors) with retained bioactivities, a mask-free, non-contact, biocompatible and multiphoton-based microfabrication and micropatterning method for engineering a spatially and quantitatively controllable soluble proteins/bioactive factors and cell-cell adhesion molecules. An universal cell niche engineering platform is provided that contributes to reconstituting heterogeneous native soluble cell niche for signal transduction modeling and drug screening studies.
Inventors:
CHAN PUI BARBARA (CN)
YIP CHI HUNG (CN)
CHEN ABIGAIL DEE (CN)
WANG XINNA (CN)
HUANG NAN (CN)
YIP CHI HUNG (CN)
CHEN ABIGAIL DEE (CN)
WANG XINNA (CN)
HUANG NAN (CN)
Application Number:
PCT/CN2022/075402
Publication Date:
August 11, 2022
Filing Date:
February 07, 2022
Export Citation:
Assignee:
UNIV HONG KONG (CN)
International Classes:
C40B40/00; C12M1/32; C12M3/00; C12N5/00; C12N5/02; C12Q1/02; G01N33/53
Foreign References:
| US20180066299A1 | 2018-03-08 | |||
| US20060160066A1 | 2006-07-20 |
Other References:
WANG XINNA; GAO BO; CHAN BARBARA P.: "Multiphoton microfabrication and micropatterning (MMM) – An all-in-one platform for engineering biomimetic soluble cell niches", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 269, 5 January 2021 (2021-01-05), AMSTERDAM, NL , XP086485559, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2020.120644
HUANG NAN, LI CHUEN WAI, CHAN BARBARA PUI: "Multiphoton 3D Microprinting of Protein Micropatterns with Spatially Controlled Heterogeneity - A Platform for Single Cell Matrix Niche Studies", ADVANCED BIOSYSTEMS, vol. 2, no. 8, 1 August 2018 (2018-08-01), pages 1800053, XP055956109, ISSN: 2366-7478, DOI: 10.1002/adbi.201800053
ZHANG，Y. ET AL.: "Cadherin-based biomaterials: Inducing stem cell fate towards tissue construction and therapeutics.", PROGRESS IN NATURAL SCIENCE: MATERIALS INTERNATIONAL., vol. 30, 22 September 2020 (2020-09-22), pages 597 - 608, XP086421203, DOI: 10.1016/j.pnsc.2020.09.001
TONG MING HUI, HUANG NAN, ZHANG WEI, ZHOU ZHUO LONG, NGAN ALFONSO HING WAN, DU YANAN, CHAN BARBARA PUI: "Multiphoton photochemical crosslinking-based fabrication of protein micropatterns with controllable mechanical properties for single cell traction force measurements", SCIENTIFIC REPORTS, vol. 6, no. 1, 1 April 2016 (2016-04-01), XP055956113, DOI: 10.1038/srep20063
MA，J.N. ET AL.: "Multiphoton Fabrication of Fibronectin-Functionalized Protein Micropatterns: Stiffness-Induced Maturation of Cell-Matrix Adhesions in Human Mesenchymal Stem Cells.", ACS APPL MATER INTERFACES., vol. 9, 15 August 2017 (2017-08-15), pages 29469 - 29480, XP55956116
TONG MING HUI, HUANG NAN, NGAN ALFONSO HING WAN, DU YANAN, CHAN BARBARA PUI: "Preferential sensing and response to microenvironment stiffness of human dermal fibroblast cultured on protein micropatterns fabricated by 3D multiphoton biofabrication", SCIENTIFIC REPORTS, vol. 7, no. 1, 1 December 2017 (2017-12-01), XP055956120, DOI: 10.1038/s41598-017-12604-z
HUANG NAN, LI CHUEN WAI, CHAN BARBARA PUI: "Multiphoton 3D Microprinting of Protein Micropatterns with Spatially Controlled Heterogeneity - A Platform for Single Cell Matrix Niche Studies", ADVANCED BIOSYSTEMS, vol. 2, no. 8, 1 August 2018 (2018-08-01), pages 1800053, XP055956109, ISSN: 2366-7478, DOI: 10.1002/adbi.201800053
ZHANG，Y. ET AL.: "Cadherin-based biomaterials: Inducing stem cell fate towards tissue construction and therapeutics.", PROGRESS IN NATURAL SCIENCE: MATERIALS INTERNATIONAL., vol. 30, 22 September 2020 (2020-09-22), pages 597 - 608, XP086421203, DOI: 10.1016/j.pnsc.2020.09.001
TONG MING HUI, HUANG NAN, ZHANG WEI, ZHOU ZHUO LONG, NGAN ALFONSO HING WAN, DU YANAN, CHAN BARBARA PUI: "Multiphoton photochemical crosslinking-based fabrication of protein micropatterns with controllable mechanical properties for single cell traction force measurements", SCIENTIFIC REPORTS, vol. 6, no. 1, 1 April 2016 (2016-04-01), XP055956113, DOI: 10.1038/srep20063
MA，J.N. ET AL.: "Multiphoton Fabrication of Fibronectin-Functionalized Protein Micropatterns: Stiffness-Induced Maturation of Cell-Matrix Adhesions in Human Mesenchymal Stem Cells.", ACS APPL MATER INTERFACES., vol. 9, 15 August 2017 (2017-08-15), pages 29469 - 29480, XP55956116
TONG MING HUI, HUANG NAN, NGAN ALFONSO HING WAN, DU YANAN, CHAN BARBARA PUI: "Preferential sensing and response to microenvironment stiffness of human dermal fibroblast cultured on protein micropatterns fabricated by 3D multiphoton biofabrication", SCIENTIFIC REPORTS, vol. 7, no. 1, 1 December 2017 (2017-12-01), XP055956120, DOI: 10.1038/s41598-017-12604-z
Attorney, Agent or Firm:
LIU, SHEN & ASSOCIATES (CN)
Download PDF: