Title:
CELL POPULATION INCLUDING MESENCHYMAL STEM CELLS, CELL SHEET, AND CELL SHEET PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2023/090346
Kind Code:
A1
Abstract:
The present invention obtains a cell population containing mesenchymal stem cells that have excellent proliferative ability and osteoblast differentiation ability and that are useful for regeneration of periodontal tissues. Further, provided are: a cell sheet in which the material thereof is a cell population containing mesenchymal stem cells that have excellent proliferative ability and osteoblast differentiating ability and that are useful for regeneration of periodontal tissues; and a production method for the cell sheet. The present invention involves acquiring, through selection, a cell population having an alkaline phosphatase activity of 1 U or more before undergoing calcification induction, from a cell population containing mesenchymal stem cells derived from a tooth or a periodontal tissue. The cell sheet is obtained by: (a) a step for acquiring a cell population that contains mesenchymal stem cells having an alkaline phosphatase activity; (b) a step for culturing the cell population in a calcification induction medium and growing same on a plastic substrate; and (c) a step for recovering and rinsing a sheet-like cell population formed on the plastic substrate.
Inventors:
IWATA TAKANORI (JP)
ONIZUKA SATORU (JP)
ONIZUKA SATORU (JP)
Application Number:
PCT/JP2022/042503
Publication Date:
May 25, 2023
Filing Date:
November 16, 2022
Export Citation:
Assignee:
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
International Classes:
C12N5/0775
Domestic Patent References:
| WO2017073656A1 | 2017-05-04 |
Foreign References:
| JP2010252778A | 2010-11-11 | |||
| JP2011520434A | 2011-07-21 |
Other References:
MORITANI Y., USUI M., SANO K., NAKAZAWA K., HANATANI T., NAKATOMI M., IWATA T., SATO T., ARIYOSHI W., NISHIHARA T., NAKASHIMA K.: "Spheroid culture enhances osteogenic potential of periodontal ligament mesenchymal stem cells", JOURNAL OF PERIODONTAL RESEARCH, MUNKSGAARD., HOBOKEN, USA, vol. 53, no. 5, 1 October 2018 (2018-10-01), Hoboken, USA, pages 870 - 882, XP093067399, ISSN: 0022-3484, DOI: 10.1111/jre.12577
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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