Title:
CELL PRESERVATION SOLUTION AND CELL PRESERVATION METHOD
Document Type and Number:
WIPO Patent Application WO/2024/026996
Kind Code:
A1
Abstract:
One aspect of the embodiments relates to a cell preservation solution, comprising: 20-50 mM of sucrose; 20-50 mM of mannitol; 10-50 mM of lactobionic acid; 5-20 mM of glucose; 1-3 mM of adenosine; 1-5 mM of reduced glutathione; 1-2 mM of dextran-40; 10-30 mM of potassium dihydrogen phosphate; 10-30 mM of potassium carbonate; 10-30 mM of potassium chloride; 5-30 mM of sodium chloride; 10-60 mM of sodium hydroxide; 10-60 mM of potassium hydroxide; 0-20 mM of vitamin E; 0-25 mM of 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid; and 0-10% of dimethyl sulfoxide. Another aspect of the embodiments relates to a cell preservation method, which uses the cell preservation solution of the present application to store cells.
Inventors:
TANG WEI (CN)
Application Number:
PCT/CN2022/120197
Publication Date:
February 08, 2024
Filing Date:
September 21, 2022
Export Citation:
Assignee:
UNIDT SHANGHAI CO LTD (CN)
International Classes:
A01N1/02
Foreign References:
| CN114457003A | 2022-05-10 | |||
| CN109845728A | 2019-06-07 | |||
| CN113015794A | 2021-06-22 | |||
| CN113056557A | 2021-06-29 | |||
| CN113490414A | 2021-10-08 | |||
| US20120076854A1 | 2012-03-29 |
Other References:
JITRARUCH SUTTIRUK, DHAWAN ANIL, HUGHES ROBIN D., FILIPPI CELINE, LEHEC SHARON C., GLOVER LEANNE, MITRY RAGAI R.: "Cryopreservation of Hepatocyte Microbeads for Clinical Transplantation", CELL TRANSPLANTATION, SAGE, US, vol. 26, no. 8, 1 August 2017 (2017-08-01), US , pages 1341 - 1354, XP093136673, ISSN: 0963-6897, DOI: 10.1177/0963689717720050
LI YU, WANG MIN, SONG BOYU, LI HAO, FANG QIAN, DAI GUICHAO, HU JIANHONG: " Research Progress of Cryopreservation of Goat Semen", JOURNAL OF DOMESTIC ANIMAL ECOLOGY, vol. 40, no. 7, 1 July 2019 (2019-07-01), pages 82 - 86, XP093136694
LI YU, WANG MIN, SONG BOYU, LI HAO, FANG QIAN, DAI GUICHAO, HU JIANHONG: " Research Progress of Cryopreservation of Goat Semen", JOURNAL OF DOMESTIC ANIMAL ECOLOGY, vol. 40, no. 7, 1 July 2019 (2019-07-01), pages 82 - 86, XP093136694
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW CO., LTD. (CN)
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