Title:
CELL PURIFICATION METHOD
Document Type and Number:
WIPO Patent Application WO/2023/074873
Kind Code:
A1
Abstract:
Provided is a highly precise cell purification method which can be carried out without using a cell sorting device such as a flow cytometer, and whereby the influence of cell types is minimized. A target cell purification method according to the present invention includes: a step for introducing, to a cell population, first mRNA which is miRNA-responsive OFF switch mRNA encoding a lethal gene or anti-lethal gene, second mRNA which is miRNA-responsive ON switch mRNA encoding an anti-lethal gene that deactivates the lethal gene or a lethal gene that is deactivated by the anti-lethal gene, and third mRNA encoding a drug-resistant gene; and a step for culturing the cell population in the presence of a drug.
Inventors:
SAITO HIROHIDE (JP)
FUJITA YOSHIHIKO (JP)
HIROSAWA MOE (JP)
MASAKI KAITO (JP)
FUJITA YOSHIHIKO (JP)
HIROSAWA MOE (JP)
MASAKI KAITO (JP)
Application Number:
PCT/JP2022/040488
Publication Date:
May 04, 2023
Filing Date:
October 28, 2022
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N5/10; A61K35/12; A61P35/00; C12N15/113; C12Q1/04; C12Q1/68
Domestic Patent References:
| WO2015105172A1 | 2015-07-16 | |||
| WO2018003779A1 | 2018-01-04 | |||
| WO2016010119A1 | 2016-01-21 | |||
| WO2015141827A1 | 2015-09-24 |
Attorney, Agent or Firm:
OKUYAMA, Shoichi et al. (JP)
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