Title:
CELL RELEASE METHOD, CELL RELEASE SOLUTION, CELL CULTURE METHOD, CELL CULTURE MEDIUM, CELL SOLUTION, CELL SOLUTION PREPARATION, CELL COLONIZATION METHOD AND CELL COLONIZATION SOLUTION
Document Type and Number:
WIPO Patent Application WO/2006/109367
Kind Code:
A1
Abstract:
[PROBLEMS] It is intended to provide a method for culturing and proliferating an adhesive animal cell normally in a floating state and colonizing the cultured and proliferated adhesive animal cells in a desired place again. [MEANS FOR SOLVING PROBLEMS] A cell release method according to the invention is characterized by adding lactoferrin to a culture medium in a fermentor in which the cells are cultured. A cell culture method according to the invention is characterized by performing suspension culturing of a cell in a culture medium containing lactoferrin. The cell colonization method according to the invention is characterized by diluting lactoferrin, transferring the culture medium containing floating cells to a fermentor coated with laminin, or adding glycosaminoglycan. In these methods, the cell is an adhesive animal cell and serum is contained in the culture medium.
Inventors:
ISHII TOSHIAKI (JP)
Application Number:
PCT/JP2006/300698
Publication Date:
October 19, 2006
Filing Date:
January 19, 2006
Export Citation:
Assignee:
EDICINE OBIHIRO UNIVERSITY OF (JP)
ISHII TOSHIAKI (JP)
ISHII TOSHIAKI (JP)
International Classes:
C12N5/00; A61K38/16; C12N5/02
Foreign References:
| JPH05502385A | 1993-04-28 | |||
| JP2947488B2 | 1999-09-13 |
Other References:
ISHIMORI Y. ET AL.: "Bovine Lactoferrin wa Maku Ketsugogata Proteoglycan o Kaishite PC12 Saibo no Secchakuno ni Eikyo Suru", DAI 139 KAI JAPANESE SOCIETY OF VETERIANY SCIENCE GAKUJUTSU SHUKAI KOEN YOSHISHU, 1 March 2005 (2005-03-01), pages 226 (JP-101), XP003002082
Attorney, Agent or Firm:
Kubota, Noriaki (Higashigotanda 1-chome Shinagawa-k, Tokyo 22, JP)
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