Title:
CHITIN BEADS, CHITOSAN BEADS, PROCESS FOR PREPARING THESE BEADS, CARRIER COMPRISING SAID BEADS, AND PROCESS FOR PREPARING MICROSPORIDIAN SPORE
Document Type and Number:
WIPO Patent Application WO/1998/051711
Kind Code:
A1
Abstract:
Chitin beads having a uniform fine particle diameter and comprising a microsporidian spore whose main cell wall material is chitin; chitosan beads having a uniform fine particle diameter and prepared by N-deacetylating the chitin; a process for preparing the beads; a carrier comprising the beads; and a process for preparing a microsporidian spore. A microsporidian spore is propagated in an insect or a culture cell to prepare chitin beads having a uniform fine particle diameter and containing chitin as the main cell wall material. The chitin as the cell wall material is N-deacetylated to prepare chitosan beads. When the insect is the larva of a domesticated silkworm, the microsporidian spore is orally or percutaneously inoculated in a concentration of 5 x 10?2� to 5 x 10?8�/ml into the larva of a second-instar exuviated domesticated silkworm to effect propagation. The propagated microsporidian spore is taken out of the grown fifth-instar larva body and purified to prepare chitin beads having a uniform fine particle diameter and chitosan beads are prepared therefrom in the same manner as that described above. These beads are used as a carrier for immobilizing or encapsulating various materials such as antibiotics and microorganisms.
Inventors:
TSUKADA MASUHIRO (JP)
SHIRATA AKIRA (JP)
HAYASAKA SHOJI (JP)
SHIRATA AKIRA (JP)
HAYASAKA SHOJI (JP)
Application Number:
PCT/JP1997/003741
Publication Date:
November 19, 1998
Filing Date:
October 16, 1997
Export Citation:
Assignee:
JP NATIONAL INST OF SERICULTUR (JP)
TSUKADA MASUHIRO (JP)
SHIRATA AKIRA (JP)
HAYASAKA SHOJI (JP)
TSUKADA MASUHIRO (JP)
SHIRATA AKIRA (JP)
HAYASAKA SHOJI (JP)
International Classes:
A01N25/10; A01N25/26; A23L5/00; A23L27/00; A23L29/275; A61K9/16; C09B67/02; C08B37/08; (IPC1-7): C08B37/08; C12P19/26; C12N1/10; C12N11/10; A23L1/00; A61K9/50; A61K47/36; A01N25/10; C11B9/00; C09B67/02; A23K1/16
Domestic Patent References:
| WO1993014216A1 | 1993-07-22 |
Foreign References:
| JPH0725905A | 1995-01-27 | |||
| JPH04132701A | 1992-05-07 | |||
| JPS50116632A | 1975-09-12 |
Attorney, Agent or Firm:
Kitamura, Kinichi (16-1 Shinbashi 2-chom, Minato-ku Tokyo 105, JP)
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