Title:
COMBINATION OF PEPTIDE LINKERS FOR PROTEIN COVALENT SELF-ASSEMBLY USING SPONTANEOUS ISOPEPTIDE BOND
Document Type and Number:
WIPO Patent Application WO/2022/222700
Kind Code:
A1
Abstract:
Disclosed is a combination of peptide linkers for protein covalent self-assembly using a spontaneous isopeptide bond, comprising a peptide linker 1 and a peptide linker 2. The peptide linker 1 contains a peptide chain having an amino acid sequence as shown in SEQ ID NO: 5, and the peptide linker 2 contains any one or more of peptide chains having amino acid sequences as shown in SEQ ID NOs: 11, 13, 14, and 46. The present invention constructs a novel protein self-assembly system based on an isopeptide bond, which effectively solves the problem of low binding efficiency of an existing SpyTag/SpyCatcher system, can significantly improve the reaction efficiency of molecules or components fused to a peptide tag and polypeptide thereof, and has a binding efficiency stronger than that of a SpyTag003/SpyCatcher003 system. Moreover, a GvTagOpti/SdCatcher system, a PsTag/SdCatcher system, and a SaTag/SdCatcher system of the present invention have a simple preparation method and a high protein expression level.
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Inventors:
ZHANG HUI (CN)
ZHANG XIANTAO (CN)
ZHANG XIANTAO (CN)
Application Number:
PCT/CN2022/083430
Publication Date:
October 27, 2022
Filing Date:
March 28, 2022
Export Citation:
Assignee:
UNIV SUN YAT SEN (CN)
International Classes:
C07K14/00; C07K1/00; C07K7/08; C07K19/00
Domestic Patent References:
| WO2011098772A1 | 2011-08-18 | |||
| WO2018197854A1 | 2018-11-01 | |||
| WO2016193746A1 | 2016-12-08 |
Foreign References:
| CN113621031A | 2021-11-09 |
Other References:
DATABASE NUCLEOTIDE 28 October 2019 (2019-10-28), ANONYMOUS : "Streptococcus dysgalactiae strain GCH3 10, whole genome shotgun sequence", XP055978500, retrieved from NCBI Database accession no. WHSD01000010
DATABASE PROTEIN 11 April 2022 (2022-04-11), ANONYMOUS : "SpaA isopeptide-forming pilin-related protein [Gardnerella vaginalis]", XP055978506, retrieved from NCBI Database accession no. WP_165847589
DATABASE PROTEIN 31 October 2016 (2016-10-31), ANONYMOUS : "Cna protein B-type domain-containing protein, partial [Peptostreptococcus sp. D1]", XP055978510, retrieved from NCBI Database accession no. SFE28504
DATABASE PROTEIN 11 April 2022 (2022-04-11), ANONYMOUS : "SpaA isopeptide-forming pilin-related protein [Streptococcus anginosus]", XP055978518, retrieved from NCBI Database accession no. WP_020999873
DATABASE PROTEIN 11 April 2022 (2022-04-11), ANONYMOUS : "SpaA isopeptide-forming pilin-related protein [Gardnerella vaginalis]", XP055978506, retrieved from NCBI Database accession no. WP_165847589
DATABASE PROTEIN 31 October 2016 (2016-10-31), ANONYMOUS : "Cna protein B-type domain-containing protein, partial [Peptostreptococcus sp. D1]", XP055978510, retrieved from NCBI Database accession no. SFE28504
DATABASE PROTEIN 11 April 2022 (2022-04-11), ANONYMOUS : "SpaA isopeptide-forming pilin-related protein [Streptococcus anginosus]", XP055978518, retrieved from NCBI Database accession no. WP_020999873
Attorney, Agent or Firm:
YOGO PATENT AND TRADEMARK AGENCY LIMITED COMPANY (CN)
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