Title:
COMPOSITION FOR INHIBITING PREADIPOCYTE DIFFERENTIATION AND LIPID ACCUMULATION COMPRISING ENTEROCOCCUS FAECALIS, CULTURE FLUID THEREOF, OR KILLED CELLS THEREOF AS ACTIVE INGREDIENT
Document Type and Number:
WIPO Patent Application WO/2023/153902
Kind Code:
A1
Abstract:
The present invention relates to a composition for inhibiting preadipocyte differentiation and lipid accumulation, comprising Enterococcus faecalis as an active ingredient. Particularly, it was confirmed that killed E. faecalis EF-2001 induces the cell cycle delay of differentiation-induced preadipocytes, induces downregulation of the phosphorylation of downstream signals, such as Akt, Erk, and JNK, in the insulin receptor signaling pathway, has an effect of reducing the protein expression levels of C/EBP-α and PPAR-γ, and has an effect of reducing the amounts of total cholesterol, triglyceride, and non-high density cholesterol in an experimental animal having obesity induced by a high-fat diet. Thus, the E. faecalis, a culture fluid thereof, or killed cells thereof may be used as an active ingredient in the composition for inhibiting preadipocyte differentiation and lipid accumulation.
Inventors:
KIM TACK JOONG (KR)
LEE JIN HO (KR)
LEE JIN HO (KR)
Application Number:
PCT/KR2023/002084
Publication Date:
August 17, 2023
Filing Date:
February 13, 2023
Export Citation:
Assignee:
UNIV INDUSTRY FOUNDATION YONSEI UNIV WONJU CAMPUS (KR)
International Classes:
A61K35/744; A23L33/135; A61K8/99; A61P3/06; A61P9/04; A61P9/10; A61Q19/00
Other References:
HYUN IN KYUNG, LEE JI SOO, YOON JI-WON, KANG SEOK-SEONG: "Skimmed milk fermented by lactic acid bacteria inhibits adipogenesis in 3T3-L1 pre-adipocytes by downregulating PPARγ via TNF-α induction in vitro", FOOD & FUNCTION, R S C PUBLICATIONS, GB, vol. 12, no. 18, 20 September 2021 (2021-09-20), GB , pages 8605 - 8614, XP093084659, ISSN: 2042-6496, DOI: 10.1039/D1FO00076D
KONDOH MASATOSHI, SHIMADA TAKASHI, FUKADA KAZUTAKE, MORITA MAYUKO, KATADA KAZUHIRO, HIGASHIMURA YASUKI, MIZUSHIMA KATSURA, OKAMORI: "Beneficial effects of heat-treated Enterococcus faecalis FK-23 on high-fat diet-induced hepatic steatosis in mice", BRITISH JOURNAL OF NUTRITION, UK, vol. 112, no. 6, 28 September 2014 (2014-09-28), UK , pages 868 - 875, XP093084660, ISSN: 0007-1145, DOI: 10.1017/S0007114514001792
GAO YURONG; LI DAPENG: "Screening of lactic acid bacteria with cholesterol-lowering and triglyceride-lowering activity in vitro and evaluation of probiotic function", ANALS OF MICROBIOLOGY, DISTAM, MILAN, IT, vol. 68, no. 9, 2 August 2018 (2018-08-02), IT , pages 537 - 545, XP036573928, ISSN: 1590-4261, DOI: 10.1007/s13213-018-1360-0
LEE JIN-HO, WOO KEUN-JUNG, KIM MIN-AH, HONG JOONPYO, KIM JIHEE, KIM SUN-HONG, HAN KWON-IL, IWASA MASAHIRO, KIM TACK-JOONG: "Heat-Killed Enterococcus faecalis Prevents Adipogenesis and High Fat Diet-Induced Obesity by Inhibition of Lipid Accumulation through Inhibiting C/EBP-α and PPAR-γ in the Insulin Signaling Pathway", NUTRIENTS, vol. 14, no. 6, 20 March 2022 (2022-03-20), pages 1308, XP093084665, DOI: 10.3390/nu14061308
FAN MEIQI, CHOI YOUNG-JIN, WEDAMULLA NISHALA ERANDI, TANG YUJIAO, HAN KWON IL, HWANG JI-YOUNG, KIM EUN-KYUNG: "Heat-Killed Enterococcus faecalis EF-2001 Attenuate Lipid Accumulation in Diet-Induced Obese (DIO) Mice by Activating AMPK Signaling in Liver", FOODS, vol. 11, no. 4, 16 February 2022 (2022-02-16), pages 575, XP093084674, DOI: 10.3390/foods11040575
KONDOH MASATOSHI, SHIMADA TAKASHI, FUKADA KAZUTAKE, MORITA MAYUKO, KATADA KAZUHIRO, HIGASHIMURA YASUKI, MIZUSHIMA KATSURA, OKAMORI: "Beneficial effects of heat-treated Enterococcus faecalis FK-23 on high-fat diet-induced hepatic steatosis in mice", BRITISH JOURNAL OF NUTRITION, UK, vol. 112, no. 6, 28 September 2014 (2014-09-28), UK , pages 868 - 875, XP093084660, ISSN: 0007-1145, DOI: 10.1017/S0007114514001792
GAO YURONG; LI DAPENG: "Screening of lactic acid bacteria with cholesterol-lowering and triglyceride-lowering activity in vitro and evaluation of probiotic function", ANALS OF MICROBIOLOGY, DISTAM, MILAN, IT, vol. 68, no. 9, 2 August 2018 (2018-08-02), IT , pages 537 - 545, XP036573928, ISSN: 1590-4261, DOI: 10.1007/s13213-018-1360-0
LEE JIN-HO, WOO KEUN-JUNG, KIM MIN-AH, HONG JOONPYO, KIM JIHEE, KIM SUN-HONG, HAN KWON-IL, IWASA MASAHIRO, KIM TACK-JOONG: "Heat-Killed Enterococcus faecalis Prevents Adipogenesis and High Fat Diet-Induced Obesity by Inhibition of Lipid Accumulation through Inhibiting C/EBP-α and PPAR-γ in the Insulin Signaling Pathway", NUTRIENTS, vol. 14, no. 6, 20 March 2022 (2022-03-20), pages 1308, XP093084665, DOI: 10.3390/nu14061308
FAN MEIQI, CHOI YOUNG-JIN, WEDAMULLA NISHALA ERANDI, TANG YUJIAO, HAN KWON IL, HWANG JI-YOUNG, KIM EUN-KYUNG: "Heat-Killed Enterococcus faecalis EF-2001 Attenuate Lipid Accumulation in Diet-Induced Obese (DIO) Mice by Activating AMPK Signaling in Liver", FOODS, vol. 11, no. 4, 16 February 2022 (2022-02-16), pages 575, XP093084674, DOI: 10.3390/foods11040575
Attorney, Agent or Firm:
KIM, Bo Jung (KR)
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