Title:
CONSTRUCTION METHOD OF ENGINEERING BACTERIA AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/108503
Kind Code:
A1
Abstract:
Provided are a construction method of engineering bacteria, engineering bacteria obtained by means of construction and use of same in the preparation of sclareol. The construction method comprises the following steps: a DNA fragment I which comprises a promoter, a fusion gene and a terminator in sequence from upstream to bottom is introduced into a saccharomyces cerevisiae host strain. The fusion gene comprises in sequence from upstream to bottom: a TPS gene, a coding gene for a linker peptide I and an LPPS gene. The construction method optimizes the sclareol synthetase of the host strain, thereby facilitating an increase in sclareol yield.
Inventors:
ZHOU YONGJIN (CN)
CAO XUAN (CN)
CAO XUAN (CN)
Application Number:
PCT/CN2021/138506
Publication Date:
June 22, 2023
Filing Date:
December 15, 2021
Export Citation:
Assignee:
DALIAN INST CHEM & PHYSICS CAS (CN)
International Classes:
C12N15/52; C12N1/19; C12N15/62; C12P7/02; C12R1/865
Foreign References:
| CN103387944A | 2013-11-13 | |||
| CN112574897A | 2021-03-30 | |||
| CN108085262A | 2018-05-29 | |||
| US20140162332A1 | 2014-06-12 |
Other References:
YANG, WEI ET AL.: "Engineering Saccharomyces Cerevisiae for Sclareol Production", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 29, no. 8, 25 August 2013 (2013-08-25), XP009547105, ISSN: 1000-3061
MICHEL SCHALK ET AL.: "Toward a biosynthetic route to sclareol and amber odorants", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 134, no. 46, 21 November 2012 (2012-11-21), XP002724077, ISSN: 0002-7863, DOI: 10.1021/JA.307404U
MICHEL SCHALK ET AL.: "Toward a biosynthetic route to sclareol and amber odorants", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 134, no. 46, 21 November 2012 (2012-11-21), XP002724077, ISSN: 0002-7863, DOI: 10.1021/JA.307404U
Attorney, Agent or Firm:
PERIODIC LAW FIRM (CN)
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