Title:
METHOD FOR INCREASING SUPPLY OF YEAST STRAIN NADPH AND FADH 2, ENGINEERING BACTERIA, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/108504
Kind Code:
A1
Abstract:
A method for increasing the supply of yeast strain NADPH and FADH 2, engineering bacteria, and an application thereof. The method comprises the enhancement of NADPH regeneration and the enhancement of intracellular FADH 2 supply. The enhancement of NADPH regeneration comprises: introducing an LmXFPK gene, a CkPTA gene, a TAL1 gene and a TKL1 gene into the yeast strain. The enhancement of intracellular FADH 2 supply comprises: introducing an RIB1 gene and an FLX1 gene into the yeast strain. The problem of insufficient supply of endogenous cofactors in the synthesis process of phenolic acid and the like can be solved, thereby improving the synthesis efficiency of NADPH/FADH 2-dependent natural products such as phenolic acid.
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Inventors:
ZHOU YONGJIN (CN)
ZHANG LEI (CN)
CHEN RUIBING (CN)
ZHANG LEI (CN)
CHEN RUIBING (CN)
Application Number:
PCT/CN2021/138510
Publication Date:
June 22, 2023
Filing Date:
December 15, 2021
Export Citation:
Assignee:
DALIAN INST CHEM & PHYSICS CAS (CN)
International Classes:
C12N1/16; C12N1/18; C12N1/19; C12N15/52; C12N15/81; C12P7/42
Domestic Patent References:
| WO2021089961A1 | 2021-05-14 |
Foreign References:
| CN111778167A | 2020-10-16 |
Other References:
RUIBING CHEN, SHAN YANG, LEI ZHANG, YONGJIN J. ZHOU: "Advanced Strategies for Production of Natural Products in Yeast", ISCIENCE, ELSEVIER, 27 March 2020 (2020-03-27), pages 100879, XP055711914, Retrieved from the Internet DOI: 10.1016/j.isci.2020.100879
Attorney, Agent or Firm:
PERIODIC LAW FIRM (CN)
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